...Timothy M. E. Scales; Shen Lin; Michaela Kraus; Robert G. Goold; Phillip R. Gordon-Weeks MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. We used mass spectrometry to map 28 phosphorylation sites...

...Robert G. Goold; Phillip R. Gordon-Weeks In recent studies we have demonstrated that glycogen synthase kinase 3β (GSK3β) and its substrate microtubule-associated protein 1B (MAP1B) regulate the microtubule cytoskeleton during axon outgrowth. To further examine the role GSK3β plays in axon outgrowth...

... of MAP1B high in serine-proline motifs that is phosphorylated by GSK3β. Here we show that COS cells, transiently transfected with both MAP1B and GSK3β, express high levels of the phosphorylated isoform of MAP1B (MAP1B-P) generated by GSK3β. To investigate effects of MAP1B-P on microtubule dynamics, double...

... of stimulating axonal extension and promoting MAP1b phosphorylation ( DiTella et al., 1996 ), accelerates the redistribution of cdk5 to the axonal tip and dramatically increases its activity. Finally, our results indicate that cdk5 suppression by antisense oligonucleotide treatment selectively reduces axonal...

... protein 1A (MAP1A). We have searched for a microtubule-binding domain by expressing different protein regions of MAP1A in cultured cell lines using cDNA constructs. One construct included an area with homology to the microtubule-binding domain of MAP1B ( Noble et al. (1989) J. Cell Biol . 109, 437-448...

... their possible association with centrosomes. By immuno-fluorescence, monoclonal and polyclonal antibodies raised against MAP1B stain the centrosome in cultured cells as well as purified centrosomes, whereas antibodies raised against MAP2 give a completely negative reaction. The MAP1B-related antigen is localized...

...Reiko Takemura; Shigeo Okabe; Takashige Umeyama; Yoshimitsu Kanai; Nicholas J. Cowan; Nobutaka Hirokawa ABSTRACT We previously transfected MAP2, tau and MAP1B cDNA into fibroblasts and have studied the effect of expression of these microtubule-associated proteins on microtubule organization...