... p80 patches was carried out as already described (Charette and Cosson, 2007). To visualize post-lysosome exocytosis, 10 6 cells were incubated with 10 μg/ml Alexa-Fluor-647–Dextran for 3 hours at 21°C, washed in HL5 and allowed to attach on a glass coverslip for 30 minutes. Every 10 seconds, five...