... is rare in vivo ; a notable example is the blastocyst, the lumen of which is formed by trophoblast cells ( Aziz and Alexandre, 1991 ). Fittingly, trophoblast cell lines easily form floating vesicles ( Shimada et al., 2001 ; Ezashi et al., 2011 ; Rivron et al., 2018 ), making us wonder whether...

...Julie Milanini; Racha Fayad; Mariagrazia Partisani; Patrick Lecine; Jean-Paul Borg; Michel Franco; Frédéric Luton ABSTRACT A key step of epithelial morphogenesis is the creation of the lumen. Luminogenesis by hollowing proceeds through the fusion of apical vesicles at cell–cell contacts. The small...

... to the basolateral membranes, but their sorting did not require Wls. Wnt5a-induced signaling was stimulated more efficiently at the basolateral side than the apical side of epithelial cells. Knockdown of Wnt5a delayed apical lumen formation of the epithelial cyst, and these phenotypes were rescued by wild-type Wnt5a...

... and a heptad repeat motif that is involved in the hetero-oligomeric interaction with Na,K-α 1 . We now provide evidence that knockdown of Na,K-β 1 prevents lumen formation and induces activation of extracellular regulated kinases 1 and 2 (ERK1/2) mediated by phosphatidylinositol 3-kinase in MDCK cells grown...

...Nuria Eritja; Cristina Mirantes; David Llobet; Gemma Masip; Xavier Matias-Guiu; Xavi Dolcet Most glandular tissues comprise polarized epithelial cells organized around a single central lumen. Although there is active research investigating the molecular networks involved in the regulation...

...Olga Larina; Peter Thorn In salivary acinar cells, the pattern of the Ca 2+ signals that regulates fluid and enzyme secretion has yet to be resolved, as there are conflicting reports in the literature. We have used a two-photon technique to directly visualize the acinar cell lumen in living...

...Roberto Montesano; Priscilla Soulié Lumen formation is a fundamental step in the development of the structural and functional units of glandular organs, such as alveoli and ducts. In an attempt to elucidate the molecular signals that govern this morphogenetic event, we set up an in vitro system...