... toward the nucleolus further confirmed the asymmetric properties of the nuclear envelope. ‘Localization microscopy’ relies on detection of the centroid of point-like structures, and is classically not limited by diffraction, but by the signal-to-noise ratio of individual detections. To label NPC, we...

...Anna Löschberger; Christian Franke; Georg Krohne; Sebastian van de Linde; Markus Sauer ABSTRACT Here, we combine super-resolution fluorescence localization microscopy with scanning electron microscopy to map the position of proteins of nuclear pore complexes in isolated Xenopus laevis oocyte...

...Markus Sauer Summary Super-resolution fluorescence imaging by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among...