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Keywords: Localisation
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Journal Articles
J Cell Sci (2004) 117 (21): 4979–4990.
Published: 01 October 2004
... the subcellular localisation of Cdc25B by immunofluorescence in combination with RNA interference to identify specific antibody staining. We find that endogenous Cdc25B is mainly nuclear, but a fraction resides in the cytoplasm during the G2 phase of the cell cycle. Cdc25B starts to appear in S-phase cells...
Includes: Supplementary data
Journal Articles
J Cell Sci (2004) 117 (12): 2545–2555.
Published: 15 May 2004
... be visualised in the nucleus. Furthermore, eIF4GI is localised primarily at the nuclear periphery in the vicinity of eIF4E and PABP1. Transient transfection of HeLa cells with different myc-tagged isoforms of eIF4GI did not result in any obvious differences in their localisation. However, expression of discrete...
Includes: Supplementary data
Journal Articles
J Cell Sci (2001) 114 (11): 2095–2104.
Published: 01 June 2001
... in Xenopus egg extracts. Unlike the full-length protein, the N-terminal domain shows only low affinity for paclitaxel-stabilised microtubules in vitro, but localises to the centrosomes in a microtubule-dependent manner. When expressed in Xenopus XL2 cells, it is able to target the green fluorescent protein...