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1-17 of 17
Keywords: Live imaging
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Journal Articles
Tomohito Higashi, Rachel E. Stephenson, Cornelia Schwayer, Karla Huljev, Atsuko Y. Higashi, Carl-Philipp Heisenberg, Hideki Chiba, Ann L. Miller
Journal:
Journal of Cell Science
J Cell Sci (2023) 136 (15): jcs260668.
Published: 8 August 2023
...). Imaging was started at 4 hpf and within 30 min after addition of EGTA. The most superficial epidermal layer (enveloping layer, EVL) and deep cell interface were imaged by taking z -stacks between 10–25 µm at z -steps of 0.5–1 µm at acquisition times of 0.5–6 min. For live imaging of EGTA...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
Joanna W. Pylvänäinen, Romain F. Laine, Bruno M. S. Saraiva, Sujan Ghimire, Gautier Follain, Ricardo Henriques, Guillaume Jacquemet
Journal:
Journal of Cell Science
J Cell Sci (2023) 136 (4): jcs260728.
Published: 23 February 2023
... drift correction Live imaging Image analysis ImageJ Fiji Academy of Finland http://dx.doi.org/10.13039/501100002341 338537 337531 332402 Syöpäjärjestöt http://dx.doi.org/10.13039/501100006383 Åbo Akademi http://dx.doi.org/10.13039/501100008019...
Includes: Supplementary data
Journal Articles
Tomoyuki Hatano, Tzer Chyn Lim, Ingrid Billault-Chaumartin, Anubhav Dhar, Ying Gu, Teresa Massam-Wu, William Scott, Sushmitha Adishesha, Bernardo Chapa-y-Lazo, Luke Springall, Lavanya Sivashanmugam, Masanori Mishima, Sophie G. Martin, Snezhana Oliferenko, Saravanan Palani, Mohan K. Balasubramanian
Journal:
Journal of Cell Science
J Cell Sci (2022) 135 (18): jcs260288.
Published: 23 September 2022
... (+/− N) ( Egel et al., 1994 ). The protocol for live imaging of S. pombe mating cells was adapted from Vjestica et al. (2016) . Briefly, cells were first pre-cultured overnight in MSL+N at 25°C, then diluted to OD 600 =0.05 into MSL+N and incubated at 25°C for 20 h. Exponentially growing cells were...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
Darshika Tomer, Cecilia Arriagada, Sudipto Munshi, Brianna E. Alexander, Brenda French, Pavan Vedula, Valentina Caorsi, Andrew House, Murat Guvendiren, Anna Kashina, Jean E. Schwarzbauer, Sophie Astrof
Journal:
Journal of Cell Science
J Cell Sci (2022) 135 (16): jcs260120.
Published: 25 August 2022
... of extended, periodically aligned Fn1 molecules. However, our live-imaging and single-molecule localization microscopy data are inconsistent with this traditional view and show that Fn1 fibrils are composed of roughly spherical nanodomains containing six to eleven Fn1 dimers. As they move toward the cell...
Includes: Supplementary data
Journal Articles
Anna A. Kim, Amanda Nguyen, Marco Marchetti, XinXin Du, Denise J. Montell, Beth L. Pruitt, Lucy Erin O'Brien
Journal:
Journal of Cell Science
Series: REVIEW COMMONS TRANSFER
J Cell Sci (2022) 135 (14): jcs260249.
Published: 19 July 2022
... comprise heterogeneous populations of stem and differentiated cells. Here, we examine Ca 2+ dynamics in the adult Drosophila intestine, a self-renewing epithelial organ in which stem cells continuously produce daughters that differentiate into either enteroendocrine cells or enterocytes. Live imaging...
Includes: Supplementary data
Journal Articles
A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesis
Journal:
Journal of Cell Science
Series: REVIEW COMMONS TRANSFER
J Cell Sci (2022) 135 (6): jcs259897.
Published: 30 March 2022
... Scientific, 17504044), GlutaMAX (Thermo Fisher Scientific, 35050038) and gentamicin (Thermo Fisher Scientific, 15750037). The plates were then placed in a 37°C-maintained incubator supplied with 5% CO 2 , giving time for the explants to recover for 2 h before commencing live imaging ( Das et al., 2012...
Includes: Supplementary data
Journal Articles
Yueyang Wang, Alan Y. Hsu, Eric M. Walton, Sung Jun Park, Ramizah Syahirah, Tianqi Wang, Wenqing Zhou, Chang Ding, Abby Pei Lemke, GuangJun Zhang, David M. Tobin, Qing Deng
Journal:
Journal of Cell Science
J Cell Sci (2021) 134 (8): jcs258574.
Published: 22 April 2021
... development and disease using zebrafish. However, the lack of capacity to perform gene-specific rescue or live imaging in the tissue-specific knockout background has limited the utility of this approach. Here, we report a robust and flexible gateway system for tissue-specific gene inactivation in neutrophils...
Includes: Supplementary data
Journal Articles
Jorge Luis Galeano Niño, Szun S. Tay, Jacqueline L. E. Tearle, Jianling Xie, Matt A. Govendir, Daryan Kempe, Jessica Mazalo, Alexander P. Drew, Feyza Colakoglu, Sarah K. Kummerfeld, Christopher G. Proud, Maté Biro
Journal:
Journal of Cell Science
J Cell Sci (2020) 133 (5): jcs238014.
Published: 5 March 2020
... functions are subject to translational control in addition to transcriptional regulation. Here, by using live imaging of CD8 + T cells isolated from the Lifeact-EGFP mouse, we show that T cells exhibit a gain in fluorescence intensity following engagement of cognate tumour target cells. The GFP signal...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
Journal:
Journal of Cell Science
J Cell Sci (2018) 131 (9): jcs216176.
Published: 4 May 2018
... of the mRNA encoding adenylyl cyclase A at the back of cells during this process. mRNA Live imaging Translation Cell migration Dictyostelium National Cancer Institute 10.13039/100000054 National Institutes of Health R01GM085574 Chemotaxis, the ability of cells...
Includes: Supplementary data
Journal Articles
In collection:
Cytoskeleton
Journal:
Journal of Cell Science
J Cell Sci (2017) 130 (7): 1232–1238.
Published: 1 April 2017
... and FRA1-S334E determined from live imaging experiments. N.A., not applicable because of extremely rare events of directional motility. Values are mean±s.d. Fig. 3. Analysis of FRA1 motor domain phosphorylation mutants. (A) Sequence alignment of the α-helix 5 region of the motor domains of Drosophila...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
Yanthe E. Pearson, Amanda W. Lund, Alex W. H. Lin, Chee P. Ng, Aysha Alsuwaidi, Sara Azzeh, Deborah L. Gater, Jeremy C. M. Teo
Journal:
Journal of Cell Science
J Cell Sci (2016) 129 (17): 3351–3364.
Published: 1 September 2016
..., intricately coupled with automated segmentation and multi-criterion path alignment, for a comprehensive label-free analysis of live-cell biomechanics, morphology and migration. Cell biomechanics Live imaging Mechanobiology T-lymphocyte cells Mesenchymal stem cells Cell migration Khalifa...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2013) 126 (18): 4077–4084.
Published: 15 September 2013
... that the original work is properly attributed. Live imaging Disease model WASp Zebrafish Immune deficiency Neutrophils Macrophages Cells of the innate immune system depend on a precisely regulated actin cytoskeleton to drive a coordinated response to tissue damage and infection and to clear...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2011) 124 (18): 3053–3059.
Published: 15 September 2011
... interactions between leukocytes and microbes in vivo. Using high-resolution live imaging, we found that whereas macrophages efficiently engulf bacteria from blood or fluid-filled body cavities, neutrophils barely do so. By contrast, neutrophils very efficiently sweep up surface-associated, but not fluid-borne...
Includes: Multimedia, Supplementary data
Journal Articles
Journal:
Journal of Cell Science
Series: Imaging Series
J Cell Sci (2010) 123 (21): 3629–3638.
Published: 1 November 2010
... for correspondence ( [email protected] ) © 2010. 2010 Cell adhesion Fluorescence Focal adhesion Live imaging Microscopy Migration Several different types of adhesions are formed by cells following engagement with the extracellular matrix (ECM). These structures are classified on the basis...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2009) 122 (18): 3282–3293.
Published: 15 September 2009
... myoblast fusion between mammals and Drosophila . * Author for correspondence ( [email protected] ) 18 6 2009 © The Company of Biologists Limited 2009 2009 Live imaging Cell-cell fusion Myoblast fusion Muscle Actin The formation, maintenance and growth of healthy...
Includes: Multimedia, Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2007) 120 (15): 2594–2608.
Published: 1 August 2007
Includes: Multimedia, Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1998) 111 (20): 3027–3033.
Published: 15 January 1998
.... We have used rhodamine-tubulin and live imaging techniques to study the development of asymmetries in centrosome morphology and positioning. In contrast to studies using fixed embryos, our images provide a detailed characterization of the dynamics of centrosome flattening. In addition, our...