...: The Review summarizes current knowledge about how dynein at the kinetochore contributes to efficient and accurate spindle assembly and describes the underlying molecular mechanisms. Dynein Corona Kinetochore Mitosis Spindly RZZ Nde1 Ndel1 Lis1 CENP-F Fundação para a Ciência e...

... maturation. Lis1 (also known as PAFAH1B1) is a conserved dynein regulator that is mutated in the neurodevelopmental disease lissencephaly. Here, we have combined loss-of-function studies, high-resolution imaging and separation-of-function mutants to define how Lis1 contributes to dynein-mediated corona...

... dynein co-factors Lis1 and NudE contribute to these functions remains poorly understood. Here, we show that the C. elegans NudE homolog NUD-2 is dispensable for dynein- and LIS-1-dependent mitotic spindle assembly in the zygote. This facilitates functional characterization of kinetochore-localized NUD-2...

...Hannah Murdoch; Suryakiran Vadrevu; Anke Prinz; Allan J. Dunlop; Enno Klussmann; Graeme B. Bolger; James C. Norman; Miles D. Houslay LIS1, a WD40 repeat scaffold protein, interacts with components of the cytoplasmic dynein motor complex to regulate dynein-dependent cell motility. Here, we reveal...

... hyphae, dynein localizes along microtubules and does not colocalize with abnormally accumulated early endosomes at the hyphal tip. This is in contrast to the colocalization of dynein and early endosomes in the absence of NUDF/LIS1. However, the Walker B mutation allows dynein to colocalize...

...Connie Lam; Maïlys A. S. Vergnolle; Lisa Thorpe; Philip G. Woodman; Victoria J. Allan LIS1, NDE1 and NDEL1 modulate cytoplasmic dynein function in several cellular contexts. However, evidence that they regulate dynein-dependent organelle positioning is limited. Here, we show that depletion of NDE1...

...Lotte B. Pedersen; Panteleimon Rompolas; Søren T. Christensen; Joel L. Rosenbaum; Stephen M. King Lissencephaly is a developmental brain disorder characterized by a smooth cerebral surface, thickened cortex and misplaced neurons. Classical lissencephaly is caused by mutations in LIS1 , which...