...Cecilia Alejandra Vazquez; Beatriz Escudero-Pérez; Jennifer M. Hayashi; Kristoffer E. Leon; João Paulo Moreira; Mayra Alejandra Castañeda Cataña; Allison Groseth; Melanie Ott; Lisa Oestereich; César Muñoz-Fontela; Cybele Carina Garcia; Sandra Myriam Cordo ABSTRACT Lipid droplets (LDs...

...Kazuki Ueda; Matthew N. Anderson-Baron; Julie Haskins; Sarah C. Hughes; Andrew J. Simmonds ABSTRACT Both peroxisomes and lipid droplets regulate cellular lipid homeostasis. Direct inter-organellar contacts as well as novel roles for proteins associated with peroxisome or lipid droplets occur when...

...Charles P. Najt; Mahima Devarajan; Douglas G. Mashek ABSTRACT Lipid droplets (LDs) are ubiquitous organelles that store and supply lipids for energy metabolism, membrane synthesis and production of lipid-derived signaling molecules. While compositional differences in the phospholipid monolayer...

...Toyoshi Fujimoto ABSTRACT Lipid droplets (LDs) in the cytoplasm are formed in the endoplasmic reticulum (ER) and are connected with various organelles, both structurally and functionally. This is in contrast to LDs in the nucleus, which are separated from organelles in the cytoplasm. How nuclear...

...Pawel Leznicki; Hayden O. Schneider; Jada V. Harvey; Wei Q. Shi; Stephen High ABSTRACT Membrane proteins destined for lipid droplets (LDs), a major intracellular storage site for neutral lipids, are inserted into the endoplasmic reticulum (ER) and then trafficked to LDs where they reside...

...Stéphanie Cottier; Roger Schneiter ABSTRACT Lipid droplets (LDs) are globular intracellular structures dedicated to the storage of neutral lipids. They are closely associated with the endoplasmic reticulum (ER) and are delineated by a monolayer of phospholipids that is continuous...

...Rasha Khaddaj; Muriel Mari; Stéphanie Cottier; Fulvio Reggiori; Roger Schneiter ABSTRACT Lipid droplets (LDs) are globular subcellular structures that store neutral lipids. LDs are closely associated with the endoplasmic reticulum (ER) and are limited by a phospholipid monolayer harboring...

.../ Highlighted Article: Using endogenous tagging and interaction proteomics, we identified the ER-associated lipid droplet biogenesis factor ACSL3 as a GABARAPL2-binding partner that serves as novel regulator of the enigmatic UFM1 conjugation pathway. GABARAPL2 ACSL3 Lipid droplets UBA5 Ufmylation...

...Josephine Bradley; Iestyn Pope; Yisu Wang; Wolfgang Langbein; Paola Borri; Karl Swann ABSTRACT Mammalian eggs generate most of their ATP by mitochondrial oxidation of pyruvate from the surrounding medium or from fatty acids that are stored as triacylglycerols within lipid droplets. The balance...

... of the endoplasmic reticulum (ER) that is also present at lipid droplets. Manipulations of isoform 2 levels cause rapid ER stress, likely through deregulation of lipid homeostasis, and lead to cell death. Our work highlights how alternative isoforms provide functional expansion of DUBs and sets directions for future...

...Peter J. Thul; Kirsten Tschapalda; Petra Kolkhof; Abdou Rachid Thiam; Monika Oberer; Mathias Beller ABSTRACT Lipid droplets (LDs) are the principal organelles of lipid storage. They consist of a hydrophobic core of storage lipids, surrounded by a phospholipid monolayer with proteins attached. While...

...Abdou Rachid Thiam; Mathias Beller ABSTRACT Lipid droplets are the universal cellular organelles for the transient or long-term storage of lipids. The number, size and composition of lipid droplets vary greatly within cells in a homogenous population as well as in different cell types...

... temporally is not well understood. We analyzed this issue for bidirectionally moving lipid droplets in Drosophila embryos, a system in which net transport direction is developmentally controlled. By quantifying how the droplet distribution changes as embryos develop, we characterize temporal transitions...

...Arndt Rohwedder; Qifeng Zhang; Simon A. Rudge; Michael J. O. Wakelam ABSTRACT It is unclear how changes in lipid droplet size and number are regulated – for example, it is not known whether this involves a signalling pathway or is directed by cellular lipid uptake. Here, we show that oleic acid...

... of deacetylase, kinase and phosphatase signaling activities. The localized signaling gradients established by targeted O -GlcNAc cycling influence many important cellular processes, including lipid droplet remodeling, mitochondrial functioning, epigenetic control of gene expression and proteostasis...

...Chao-Wen Wang; Yu-Hsuan Miao; Yi-Shun Chang ABSTRACT The human congenital generalized lipodystrophy type 2 protein seipin (Fld1 in budding yeast) controls lipid droplet (LD) size through an unknown mechanism. Here, we report that deletion of yeast LDB16/YCL005W , similar to deletion of FLD1...

...Nicolas Jacquier; Shirish Mishra; Vineet Choudhary; Roger Schneiter Summary Most cells store neutral lipids in a dedicated compartment, the lipid droplet (LD). These LDs are structurally and functionally conserved across species. In higher eukaryotes, LDs are covered by abundant scaffolding...

...Katharina Thiel; Christoph Heier; Verena Haberl; Peter J. Thul; Monika Oberer; Achim Lass; Herbert Jäckle; Mathias Beller Summary Lipid droplets (LDs) are specialized cell organelles for the storage of energy-rich lipids. Although lipid storage is a conserved feature of all cells and organisms...

...Chao-Wen Wang; Shu-Chuan Lee Summary Lipid droplets (LDs) are central organelles for maintaining lipid homeostasis. However, how cells control the size and number of LDs remains largely unknown. Herein, we report that Ubx2, a UBX-domain-containing protein involved in endoplasmic reticulum (ER...

... accumulates on the surface of nascent lipid droplets with perilipin-2; both of these proteins are stabilized by proteasome inhibition. We show that selective downregulation of OGA-S results in global proteasome inhibition and the striking accumulation of ubiquitinylated proteins. OGA-S knockdown increased...