...Jiwon Lee; Kai Matuschewski; Giel van Dooren; Alexander G. Maier; Melanie Rug ABSTRACT Lipid droplets (LDs) are organelles that are central to lipid and energy homeostasis across all eukaryotes. In the malaria-causing parasite Plasmodium falciparum the roles of LDs in lipid acquisition from its...

... and hepatocytes, HIG2 functions to enhance intracellular TG accumulation under hypoxic conditions. A homologous hydrophobic domain (HD) is shared by G0S2 and HIG2 (also known as HILPDA) for binding to ATGL. However, the determinants of their lipid droplet (LD) localization are unknown. Here, we study how G0S2...

... that comprise the lipid droplet (LD) proteome and reside within the limiting phospholipid monolayer of this complex organelle. We then highlight key players in the catabolic breakdown of LDs during the functionally linked lipolysis and lipophagy processes. Finally, we discuss what is currently known about macro...

... (specifically human umbilical vein endothelial cells, HUVECs) with oleic acid in the presence or absence of lactate for 6 h. Staining with free BODIPY indicated a higher lipid droplet content in ECs treated with lactate, consistent with increased fatty acid uptake and incorporation into the triglyceride pool...

..., autophagosome formation and lipid droplet expansion. In contrast to conventional syntaxins, Stx17 has a long C-terminal hydrophobic region with a hairpin-like structure flanked by a basic amino acid-enriched C-terminal tail. Although Stx17 is one of the six ancient SNAREs and is present in diverse eukaryotic...

...Tao Chen; Ahmet Yavuz; Meng C. Wang ABSTRACT Lipid droplets (LDs) are lipid-rich organelles universally found in most cells. They serve as a key energy reservoir, actively participate in signal transduction and dynamically communicate with other organelles. LD dysfunction has been associated...

...Wei Sheng Yap; Peter Shyu, Jr; Maria Laura Gaspar; Stephen A. Jesch; Charlie Marvalim; William A. Prinz; Susan A. Henry; Guillaume Thibault ABSTRACT Lipid droplets (LDs) are implicated in conditions of lipid and protein dysregulation. The fat storage-inducing transmembrane (FIT; also known as FITM...

... fattening on health. Highly related to this clinical problem, studies at the cellular and molecular level are focused on the fundamental mechanism of lipid handling in dedicated lipid droplet (LD) organelles. This Review briefly summarizes how views on LD functions have evolved from those of a specialized...

... peroxisomal membrane protein that generates the fatty alcohols required for the biosynthesis of ether lipids. Lipid droplet localization of exogenously expressed and endogenous human Far1 was observed by fluorescence microscopy under conditions of increased triglyceride synthesis in tissue culture cells...

...Susan Lassen; Cordula Grüttner; Van Nguyen-Dinh; Eva Herker ABSTRACT In hepatocytes, PLIN2 is the major protein coating lipid droplets (LDs), an organelle the hepatitis C virus (HCV) hijacks for virion morphogenesis. We investigated the consequences of PLIN2 deficiency on LDs and on HCV infection...

..., although cwh43 cells apparently consumed glucose in the culture medium. Furthermore, we found that cwh43 mutant cells had elevated levels of triacylglycerols (TGs) and coenzyme A, and that they accumulated lipid droplets. Notably, TG biosynthesis was required to maintain cell division in the cwh43 mutant...

...Sarah Ouahoud; Mitchell D. Fiet; Fernando Martínez-Montañés; Christer S. Ejsing; Oliver Kuss; Michael Roden; Daniel F. Markgraf ABSTRACT Lipid droplets (LDs) store neutral lipids and are integrated into a cellular metabolic network that relies on functional coupling with various organelles. Factors...

... lipid metabolism. We found increased lipid droplet numbers in BALB/c 3T3 cell lines stably expressing TPD52, compared with control and TPD52L1-expressing cell lines. TPD52-expressing 3T3 cells showed increased fatty acid storage in triglyceride (from both de novo synthesis and uptake) and formed greater...

...Safa Lucken-Ardjomande Häsler; Yvonne Vallis; Helen E. Jolin; Andrew N. McKenzie; Harvey T. McMahon ABSTRACT Lipid droplets are found in all cell types. Normally present at low levels in the brain, they accumulate in tumours and are associated with neurodegenerative diseases. However, little...

...Samuel Bouvet; Marie-Pierre Golinelli-Cohen; Vincent Contremoulins; Catherine L. Jackson Summary Lipid droplet metabolism and secretory pathway trafficking both require activation of the Arf1 small G protein. The spatiotemporal regulation of Arf1 activation is mediated by guanine nucleotide...

...Takeshi Hashimoto; Hiroki Segawa; Masanari Okuno; Hideaki Kano; Hiro-o Hamaguchi; Tokuko Haraguchi; Yasushi Hiraoka; Shiho Hasui; Tomohiro Yamaguchi; Fumiko Hirose; Takashi Osumi Summary The regulation of lipolysis in adipocytes involves coordinated actions of many lipid droplet (LD)-associated...

...Junfeng Bi; Yanhui Xiang; Haiyang Chen; Zhonghua Liu; Sebastian Grönke; Ronald P. Kühnlein; Xun Huang Summary Lipid droplets are the main lipid storage sites in cells. Lipid droplet homeostasis is regulated by the surface accessibility of lipases. Mammalian adipose triglyceride lipase (ATGL...

...Heimo Wolinski; Dagmar Kolb; Sandra Hermann; Roman I. Koning; Sepp D. Kohlwein Malfunctions of processes involved in cellular lipid storage and mobilization induce the pathogenesis of prevalent human diseases such as obesity, type 2 diabetes and atherosclerosis. Lipid droplets are the main lipid...

...Tanya D. Russell; Jerome Schaack; David J. Orlicky; Carol Palmer; Benny Hung-Junn Chang; Lawrence Chan; James L. McManaman Milk lipids originate by secretion of triglyceride-rich cytoplasmic lipid droplets (CLDs) from mammary epithelial cells. Adipophilin (ADPH)/Plin2, a member of the perilipin...

...John K. Zehmer; René Bartz; Blaine Bisel; Pingsheng Liu; Joachim Seemann; Richard G. W. Anderson Lipid droplets are sites of neutral lipid storage thought to be actively involved in lipid homeostasis. A popular model proposes that droplets are formed in the endoplasmic reticulum (ER) by a process...