... profilin (2 μM), tubulin (17 μM), microtubule seeds (1:750 dilution in wash buffer) and Tau (100 nM; from the laboratory of Lansky and Braun, BIOCEV, Czech Republic). The SUVs, containing lipids and lipid-biotins, were introduced in the newly micropatterned chamber and incubated for 10 min, followed...

...Yasutaka Morimoto; Shigeaki Saitoh; Yuko Takayama ABSTRACT Lipid droplets are cytoplasmic organelles that store lipids for energy and membrane synthesis. The oleaginous yeast Lipomyces starkeyi is one of the most promising lipid producers and has attracted attention as a biofuel source. It is known...

...Jordan J. Shimell; Andrea Globa; Marja D. Sepers; Angela R. Wild; Nusrat Matin; Lynn A. Raymond; Shernaz X. Bamji ABSTRACT Palmitoylation is the most common post-translational lipid modification in the brain; however, the role of palmitoylation and palmitoylating enzymes in the nervous system...

...Yan Huang; Sheng Huang; Sin Man Lam; Zhihua Liu; Guanghou Shui; Yong Q. Zhang ABSTRACT Nervous system development and function are tightly regulated by metabolic processes, including the metabolism of lipids such as fatty acids. Mutations in long-chain acyl-CoA synthetase 4 (ACSL4) are associated...

...Masashi Maekawa; Gregory D. Fairn ABSTRACT Cellular lipids play crucial roles in the cell, including in energy storage, the formation of cellular membranes, and in signaling and vesicular trafficking. To understand the functions and characteristics of lipids within cells, various methods to image...

...Alina Fridberg; Cheryl L. Olson; Ernesto S. Nakayasu; Kevin M. Tyler; Igor C. Almeida; David M. Engman Sphingolipids and their metabolites have been thought crucial for cell growth and cell cycle progression, membrane and protein trafficking, signal transduction, and formation of lipid rafts...

...Fabien Alpy; Catherine Tomasetto The steroidogenic acute regulatory protein (StAR)-related lipid transfer (START) domain is a protein module of ∼210 residues that binds lipids, including sterols. Fifteen mammalian proteins, STARD1-STARD15, possess a START domain and these can be grouped into six...

...Edward B. Cluett; Carolyn E. Machamer ABSTRACT We isolated two forms of enveloped vaccinia virus from infected HeLa cells to obtain membranes for the analysis of lipids of the cis -Golgi network and trans -Golgi network. The intracellular mature virus obtains its envelope by wrapping itself...

... cell plasma membrane, as the appearance of the invasion process in the electron microscope has been taken to suggest, or from lipid material stored in the merozoite. We have incorporated into the red cell membrane a haptenic phospholipid, phosphatidylethanolamine, containing an NBD (N-(7-nitrobenz-2...