.... Summary: The ER–mitochondria tether maintained by MFN2 fine-tunes Rac activation to regulate neutrophil adhesive migration and adhesion to endothelial cells under shear stress. Mitochondria Chemotaxis Rac Zebrafish Actin Leukocyte National Institutes of Health 10.13039/100000002...

...) Competing interests The authors declare no competing or financial interests. 29 7 2014 2 3 2015 © 2015. Published by The Company of Biologists Ltd 2015 Integrin cytoplasmic domain Inside‐out activation Talin Kindlin Platelet Leukocyte Integrins are cell surface...

... . Using genetically encoded bioprobes and high-resolution live imaging, we reveal the existence of F-actin oscillations in the trailing edge and constant repositioning of the microtubule organizing center (MTOC) to direct leukocyte migration in wounded medaka fish larvae (Oryzias latipes). Genetic...

...., 2001). Detailed study of our dynamic imaging experiments revealed several important findings. First, although the role of lateral migration has often been posited to be simply a means of getting leukocytes to the junctions to allow for paracellular diapedesis, we frequently observed T cells...

... of neutrophils with EC junctions prior to transendothelial cell migration (TEM), collectively resulting in significantly reduced extravasation. Pharmacological blockade of the leukocyte integrin MAC-1 indicated that some ICAM-2-dependent functions might be mediated through ligation of this integrin...

...-regulation of two enzymatic activities that reside in the same molecule with profound biological consequences in leukocyte cell migration. * Author for correspondence ([email protected]) 19 12 2012 © 2013. Published by The Company of Biologists Ltd 2013 HL-60 cells Cell...

...Christopher V. Carman Immune-system functions require that blood leukocytes continuously traffic throughout the body and repeatedly cross endothelial barriers (i.e. diapedese) as they enter (intravasate) and exit (extravasate) the circulation. The very earliest studies to characterize diapedesis...

... impaired. * Author for correspondence (e-mail: [email protected]) 24 10 2008 © The Company of Biologists Limited 2009 2009 Integrins JAM Leukocyte Chemotaxis JAM-A (also known as F11R or JAM1) was the first member of the junctional adhesion molecule...

... ; [email protected]) 15 2 2006 © The Company of Biologists Limited 2006 2006 Cell motility Leukocyte Dendritic cell Podosome Cell adhesion Calpain Cell locomotion involves a highly regulated cycle of assembly and disassembly of cell adhesions with the surrounding matrix...

...William D. Hanley; Denis Wirtz; Konstantinos Konstantopoulos Leukocytes are recruited from the bloodstream to sites of inflammation by the selectin family of adhesion receptors. In vivo and in vitro studies reveal distinctive rolling velocities of polymorphonuclear leukocytes over E-, P- and L...

...Ewa P. Wojcikiewicz; Xiaohui Zhang; Aileen Chen; Vincent T. Moy The interaction of leukocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) is central to the regulation of adhesion in leukocytes. In this report, we investigated the mechanisms by which phorbol...

... remodeling of the vascular wall following injury. Osteopontin has also been found to be synthesized by monocytes and macrophages within injury sites. Here, we present data showing that osteopontin can promote leukocyte adhesion through the α 4 β 1 integrin. In the presence of physiologic concentrations of Mg...

... properties, in vitro motility and rho-GTPase activity of human myosin-IXb purified from leukocytes. The results presented here indicate that myosin-IXb contains calmodulin as a light chain and that it binds to actin with high affinity in both the absence and presence of ATP. Myosin-IXb is an active motor...

...Aled Clayton; Rachel A. Evans; Elizabeth Pettit; Maurice Hallett; John D. Williams; Robert Steadman ABSTRACT In addition to its role in mediating leukocyte adherence to and migration across the endothelium, intercellular adhesion molecule (ICAM)-1 on the surface of interstitial cells has been...

... domain of protein kinase C and a putative GTPase-activating protein (GAP) domain of the rho/rac family of ras-like G-proteins. Northern blot analysis of 16 different human tissues revealed an ∼8 kb transcript that is most highly expressed in peripheral blood leukocytes, with somewhat lower levels...