.... Summary: The ER–mitochondria tether maintained by MFN2 fine-tunes Rac activation to regulate neutrophil adhesive migration and adhesion to endothelial cells under shear stress. Mitochondria Chemotaxis Rac Zebrafish Actin Leukocyte National Institutes of Health 10.13039/100000002...

... activation Talin Kindlin Platelet Leukocyte Integrins are cell surface molecules composed of α and β subunits, each containing a large extracellular domain, a short transmembrane (TM) domain, and usually a short cytoplasmic tail (CT). The combination of 18 α‐integrin and eight β‐integrin subunits...

... . Using genetically encoded bioprobes and high-resolution live imaging, we reveal the existence of F-actin oscillations in the trailing edge and constant repositioning of the microtubule organizing center (MTOC) to direct leukocyte migration in wounded medaka fish larvae ( Oryzias latipes ). Genetic...

... medium provided that the original work is properly attributed. Actin Barrier Endothelium Leukocyte Migration Stiffness The vascular endothelium represents one of the crucial barriers of the body, providing the partition between two of its key compartments – the blood circulation...

... of neutrophils with EC junctions prior to transendothelial cell migration (TEM), collectively resulting in significantly reduced extravasation. Pharmacological blockade of the leukocyte integrin MAC-1 indicated that some ICAM-2-dependent functions might be mediated through ligation of this integrin...

...-regulation of two enzymatic activities that reside in the same molecule with profound biological consequences in leukocyte cell migration. Next, to determine the physiological relevance of this model, we chose primary human neutrophils, as (a) Rac2 comprises >96% of Rac in neutrophils ( Ambruso et al...

...Christopher V. Carman Immune-system functions require that blood leukocytes continuously traffic throughout the body and repeatedly cross endothelial barriers (i.e. diapedese) as they enter (intravasate) and exit (extravasate) the circulation. The very earliest studies to characterize diapedesis...

..., the uropod of JAM-A +/+ cells was strongly elongated in a way comparable to that in JAM-A –/– cells ( Fig. 10 ). Conversely, BAPTA-AM treatment did not further modify this morphological feature in JAM-A-null cells. Altogether, these data support the idea that JAM-A promotes leukocyte directional migration...

... ). The leukocyte podosome. Eur. J. Cell Biol. 85 , 151 -157. Carragher, N. O. and Frame, M. C. ( 2002 ). Calpain: a role in cell transformation and migration. Int. J. Biochem. Cell Biol. 34 , 1539 -1543. Carragher, N. O., Fincham, V. J., Riley, D. and Frame, M. C. ( 2001 ). Cleavage of focal...

...William D. Hanley; Denis Wirtz; Konstantinos Konstantopoulos Leukocytes are recruited from the bloodstream to sites of inflammation by the selectin family of adhesion receptors. In vivo and in vitro studies reveal distinctive rolling velocities of polymorphonuclear leukocytes over E-, P- and L...

...Ewa P. Wojcikiewicz; Xiaohui Zhang; Aileen Chen; Vincent T. Moy The interaction of leukocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) is central to the regulation of adhesion in leukocytes. In this report, we investigated the mechanisms by which phorbol...