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Keywords: Lens
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Journal Articles
J Cell Sci (2018) 131 (22): jcs217240.
Published: 20 November 2018
.... ( 2000 ). Adhesion of fibronectin, vitronectin, laminin, and collagen type IV to intraocular lens materials in pseudophakic human autopsy eyes. Part 2: explanted intraocular lenses . J. Cataract Refract. Surg.   26 , 1807 - 1818 . 10.1016/S0886-3350(00)00747-1 Mamuya , F. A. , Wang , Y...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (3): 571–582.
Published: 1 February 2014
.../flox mutant lenses. n  = 10 for each genotype, One-way ANOVA test, * P <0.01. All values are means ± s.e.m. Fig. 4. Interaction of Frs2α and Shp2 is necessary for lens development. (A,B) In MEF cells, 5 days after Cre-mediated genetic deletion, residual Frs2α and Shp2 proteins were still...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (2): 198–206.
Published: 15 January 2011
...*50L or Cx43*46L were microinjected into chick lens vesicle at stage 18. The injected lenses were dissected out at embryonic day 18 and cryosections of the embedded chick lenses were immunolabeled with antibody against FLAG (green) or against AQP0 (red). The primary antibodies were detected...
Includes: Supplementary data
Journal Articles
J Cell Sci (2009) 122 (10): 1607–1615.
Published: 15 May 2009
... the large molecule diffusion pathway (LMDP). We used the tamoxifen-induced expression of GFP to investigate the nature and role of the LMDP in living, intact lenses. Our data indicate that the LMPD preferentially connects cells lying within a stratum of the lens cortex and that formation of the LMPD depends...
Includes: Multimedia, Supplementary data
Journal Articles
Journal Articles
J Cell Sci (2007) 120 (23): 4107–4116.
Published: 1 December 2007
... mutant mice. Fig. 7. Description of embryonic and adult lens phenotypes produced by mixing Cx50-S50P and wild-type lens fiber connexins. This model describes the embryonic and postnatal phenotypes of various mouse lenses created by breeding heterozygous mutant (Cx50 (S50P/+) –Cx46...
Includes: Supplementary data
Journal Articles
J Cell Sci (2003) 116 (20): 4191–4199.
Published: 15 October 2003
... suggested the co-existence of a parallel pathway. Shestopalov and Bassnett microinjected a plasmid encoding green fluorescent protein (GFP) into the lenses of embryonic chickens in ovo ( Shestopalov and Bassnett, 2000 ). When expressed in individual fiber cells in the center of the lens, GFP diffused...
Journal Articles
J Cell Sci (2003) 116 (6): 1073–1085.
Published: 15 March 2003
...Jing Hua Xi; Fang Bai; Usha P. Andley αA-Crystallin (αA) is a molecular chaperone expressed preferentially in the lens. αA transcripts are first detected during the early stages of lens development and its synthesis continues as the lens grows throughout life. αA –/– mouse lenses are smaller than...
Journal Articles
Journal Articles
J Cell Sci (2000) 113 (11): 1913–1921.
Published: 1 June 2000
... and transferred to nitrocellulose. Membranes were incubated with polyclonal anti-GFP (Molecular Probes) and bound antibody was detected by ECL (Amersham). For confocal microscopy, intact lenses or fixed lens slices were visualized using a Zeiss 410 LSM microscope equipped with an argon krypton laser...
Journal Articles
J Cell Sci (1999) 112 (13): 2155–2165.
Published: 1 July 1999
...-conjugated rabbit IgG (≈160 kDa: Jackson Immunolabs) into the lens. Lyophilized antibody was reconstituted in PBS at 1 mg/ml and dialyzed overnight against PBS to remove preservatives and unconjugated fluorochrome. E18 lenses were incubated in 100 μl of fluorescent antibody and examined by confocal...
Journal Articles
Journal Articles
J Cell Sci (1996) 109 (2): 355–365.
Published: 1 February 1996
... were removed from freshly slaughtered lambs and maintained on ice until removal of the lenses 3-7 hours later. Six lenses were placed in 6 ml of cold Hanks’ balanced salt solution without added Ca 2+ and Mg 2+ (HBSS-CMF) and, after warming to 37°C, digested with 2.5 mg/ml trypsin for 15 minutes...
Journal Articles
J Cell Sci (1995) 108 (9): 3091–3098.
Published: 1 September 1995
... bovine lenses, while unphosphorylated connexin44 only in channels purified from fetal lenses. Therefore, lens cell-to-cell channels are composed principally of equal amounts of phosphorylated connexins 44 and 50 that appear to be assembled in the same channel (‘hybrid’). * Author for correspondence...
Journal Articles
Journal Articles
J Cell Sci (1994) 107 (5): 1359–1367.
Published: 1 May 1994
... microscope using a 63× oil immersion PlanApo objective with the appropriate barrier filters and photographed using T-MAX ASA 400 film with an automatic camera. Lenses were dissected from the eyes of adult (9 week) male Lewis rats. For most experiments, the lens nucleus was removed immediately...
Journal Articles
J Cell Sci (1994) 107 (4): 799–811.
Published: 1 April 1994
... are produced subsequently by the differentiation of epithelial cells near the lens equator. Newly formed fiber cells are laid down over the pre-existing fibers. In older lenses, therefore, only the outermost fibers make direct contact with the epithelium ( Fig. 1B ). Fig. 1. Diagram illustrating...
Journal Articles
J Cell Sci (1993) 106 (1): 227–235.
Published: 1 September 1993
... the type 1 junction serves an adhesive function in the lens. Mice (CD-1, Charles River), 10-15 days old, were decapitated and the intact lenses were quickly removed from freshly enucleated eyes. Lenses were oriented so that the anterior surface (including the epithelium and fibers) or the posterior...
Journal Articles
J Cell Sci (1993) 105 (4): 1057–1068.
Published: 1 August 1993
...’ Whole lenses were dissected from 11- and 14-day embryonic chickens, fixed for 2-3 h in 2.5% paraformaldehyde in PBS, and medially transected from the lens anterior to the posterior (as suggested by W. T. M. Gruijters, University of Auckland, New Zealand). The lightly fixed central cellular masses were...
Journal Articles
J Cell Sci (1992) 103 (1): 245–257.
Published: 1 September 1992
.... The mAb 5H1 was also used to probe a Western immunoblot of lens membranes isolated from a number of different species. This antibody only recognized protein in sheep and calf lenses ( Fig. 3B , lanes 1 and 2); longer exposure of this autoradiogram revealed additional immunoreactivity in the 50-64 kDa...