... ; Findl et al., 2010 ). In the past ∼20 years, the incidence of development of PCO within 1–2 years of cataract surgery has greatly declined, due in large part to the use of intraocular lenses (IOLs) with square edges that physically block the movement of lens cells to the posterior capsule. It has...

... visible by E13.5, when the lens cavities were filled in both wild-type and mutant lenses ( Fig. 1M,N , arrows). At E14.5, the Frs2α mutant lens was reduced by 50% in size compared with the wild type ( n  = 8 controls and 7 mutants; P <0.01, Student's t test) ( Fig. 1Q,R , arrows). As we previously...

... Cx50, Cx43, Cx50*46L, Cx50*43L, Cx43*50L or Cx43*46L were microinjected into chick lens vesicle at stage 18. The injected lenses were dissected out at embryonic day 18 and cryosections of the embedded chick lenses were immunolabeled with antibody against FLAG (green) or against AQP0 (red). The primary...

... the large molecule diffusion pathway (LMDP). We used the tamoxifen-induced expression of GFP to investigate the nature and role of the LMDP in living, intact lenses. Our data indicate that the LMPD preferentially connects cells lying within a stratum of the lens cortex and that formation of the LMPD depends...

... previously ( Menko et al., 1984 ). Lenses from 10- to 11-day-old chick embryos were dissected, washed with TD buffer (140 mM NaCl, 5 mM KCl, 0.7 mM Na 2 HPO 4 , 5 mM glucose and 25 mM Tris at pH 7.4), and digested with 0.1% trypsin in TD buffer at 37°C, and then broken apart by pipetting up and down in M199...

...) with Cx46 (Cx50(S50P/S50P)–Cx46(+/+)) and (F) without endogenous Cx46 (Cx50(S50P/S50P)–Cx46(–/–)). These lenses taken from mice on postnatal day 7 showed more punctate Cx50 staining (green) which extended deeper into the lens differentiating fibers in the presence of wild-type Cx46 proteins. Frozen Lens...

... cells in the lens, a recent report suggested the co-existence of a parallel pathway. Shestopalov and Bassnett microinjected a plasmid encoding green fluorescent protein (GFP) into the lenses of embryonic chickens in ovo ( Shestopalov and Bassnett, 2000 ). When expressed in individual fiber cells...

...Jing Hua Xi; Fang Bai; Usha P. Andley αA-Crystallin (αA) is a molecular chaperone expressed preferentially in the lens. αA transcripts are first detected during the early stages of lens development and its synthesis continues as the lens grows throughout life. αA –/– mouse lenses are smaller than...

..., further analysis was only conducted on the line expressing the transgene. Fig. 1. Morphology of lenses from αA-crystallin/PAX6(5a) transgenic mice. (A) A normal lens visualized with darkfield microscopy. The faint ring around the edge is an internal reflection. (B) A lens from a transgenic mouse...

..., lenses ( Kuszak, 1995 ; Kuszak at al., 1985, 1989). Fusions have been proposed to function in the establishment of lens architecture (by providing nodal points where columns of fiber cells bifurcate; Kuszak et al., 1989 ) but the present data suggest additional physiological or metabolic roles...

... by monitoring the diffusion of a fluorescein-conjugated rabbit IgG (≈160 kDa: Jackson Immunolabs) into the lens. Lyophilized antibody was reconstituted in PBS at 1 mg/ml and dialyzed overnight against PBS to remove preservatives and unconjugated fluorochrome. E18 lenses were incubated in 100 μl of fluorescent...

... of cells isolated from the equatorial region of fresh ovine lens were prepared as described previously ( TenBroek et al., 1994 ). Briefly, eyeballs were removed from freshly slaughtered lambs and maintained on ice until removal of the lenses 3-7 hours later. 6 lenses were placed in 6 ml of Hanks’ balanced...

... of cells isolated from the equatorial region of fresh ovine lens were prepared as described previously ( TenBroek et al., 1994 ). Briefly, eyeballs were removed from freshly slaughtered lambs and maintained on ice until removal of the lenses 3-7 hours later. Six lenses were placed in 6 ml of cold Hanks...

... bovine lenses, while unphosphorylated connexin44 only in channels purified from fetal lenses. Therefore, lens cell-to-cell channels are composed principally of equal amounts of phosphorylated connexins 44 and 50 that appear to be assembled in the same channel (‘hybrid’). REFERENCES Barrio , L. C...

... that the two networks of vimentin and CP49/filensin are modulated independently during lens fibre cell differentiation and suggests complementary functions for these two filament networks. Bovine lenses were obtained from the local abattoir and within 30 minutes of slaughter were immersed in liquid...

... that there may be at least two distinct populations of actin and Tmod in the lens, which provide different cellular functions. Adult rat lenses were frozen at −20°C in embedding compound (OCT, Tissue Tek, Miles, Elkhart, IN). Sections (5 μm) were cut using a Leica-Reichert-Jung Cryocut 1800 at −15°C...

... are produced subsequently by the differentiation of epithelial cells near the lens equator. Newly formed fiber cells are laid down over the pre-existing fibers. In older lenses, therefore, only the outermost fibers make direct contact with the epithelium ( Fig. 1B ). Fig. 1. Diagram illustrating...

... the type 1 junction serves an adhesive function in the lens. Mice (CD-1, Charles River), 10-15 days old, were decapitated and the intact lenses were quickly removed from freshly enucleated eyes. Lenses were oriented so that the anterior surface (including the epithelium and fibers) or the posterior...

...% NP-40, 2 mM EDTA, 1 mM PMSF) and incubated for 1 h at 4°C. The Sepharose beads were washed several times in the same buffer and the antigen-antibody complexes were released from the beads by boiling for 5 min in SDS-PAGE loading buffer ( Laemmli, 1970 ). Lens ‘half shells’ Whole lenses...

...-junctional regions. Fig. 10. Immunofluorescence micrograph of MP70 in bovine lens cryosections. Cryosections of cortical regions of bovine lenses were labeled with the monoclonal antibody to MP70 ( Kistler et al., 1985 ) and detected with fluorescein-conjugated secondary antibody in phase-contrast...