...Imran Ullah; Umaru Barrie; Rebecca M. Kernen; Emily T. Mamula; Francis Tho Huu Khuong; Laela M. Booshehri; Emma L. Rhodes; James M. Bradford; Arani Datta; Dawn M. Wetzel ABSTRACT Leishmania spp. are obligate intracellular parasites that must be internalized by phagocytic cells to evade immune...

...Ziyin Wang; Tom Beneke; Eva Gluenz; Richard John Wheeler ABSTRACT Eukaryotic flagella undertake different beat types as necessary for different functions; for example, the Leishmania parasite flagellum undergoes a symmetric tip-to-base beat for forward swimming and an asymmetric base-to-tip beat...

...Thibault Rosazza; Hervé Lecoeur; Thierry Blisnick; Maryse Moya-Nilges; Pascale Pescher; Phillipe Bastin; Eric Prina; Gerald F. Späth ABSTRACT Leishmania spp. are obligate intracellular parasites that infect phagocytes, notably macrophages. No information is available on how Leishmania parasites...

... as the I1 complex), and the tether and tether head (T/TH) complex are thought to be key regulators of dynein action but, unlike the IDA f complex, T/TH proteins remain poorly characterised. Here, we characterised T/TH-associated proteins in the protist Leishmania mexicana . Proteome analysis of axonemes...

... the light path is split to visualise multiple focal planes simultaneously, was used to reconstruct Trypanosoma brucei and Leishmania mexicana movement in 3D. These species are uniflagellate unicellular parasites for which motility is vital. It was possible to use either a fluorescent stain or a genetically...

...Dhiman Sankar Pal; Mazharul Abbasi; Dipon Kumar Mondal; Binitha Anu Varghese; Ritama Paul; Shalini Singh; Rupak Datta ABSTRACT Leishmania parasites have evolved to endure the acidic phagolysosomal environment within host macrophages. How Leishmania cells maintain near-neutral intracellular pH...

...Dawn M. Wetzel; Emma L. Rhodes; Shaoguang Li; Diane McMahon-Pratt; Anthony J. Koleske ABSTRACT Leishmaniasis is a devastating disease that disfigures or kills nearly two million people each year. Establishment and persistence of infection by the obligate intracellular parasite Leishmania requires...

...Richard J. Wheeler; Jack D. Sunter; Keith Gull ABSTRACT Leishmania promastigote parasites have a flagellum, which protrudes from the flagellar pocket at the cell anterior, yet, surprisingly, have homologs of many flagellum attachment zone (FAZ) proteins – proteins used in the related Trypanosoma...

...Antje Hombach; Gabi Ommen; Andrea MacDonald; Joachim Clos ABSTRACT Leishmania parasites must survive and proliferate in two vastly different environments – the guts of poikilothermic sandflies and the antigen-presenting cells of homeothermic mammals. The change of temperature during...

... in the protozoan parasite Leishmania , which has been shown to be essentially required in flagellum assembly and cell motility. However, the role of this protein in cytokinesis remains largely unknown. We show here that deletion of the gene encoding ADF/cofilin in these organisms results in several aberrations...

...Judith Maxwell Silverman; Joachim Clos; Carolina Camargo de'Oliveira; Omid Shirvani; Yuan Fang; Christine Wang; Leonard J. Foster; Neil E. Reiner Specialized secretion systems are used by numerous bacterial pathogens to export virulence factors into host target cells. Leishmania and other...

...Sébastien Besteiro; Daniela Tonn; Laurence Tetley; Graham H. Coombs; Jeremy C. Mottram Lysosomal function is crucial for the differentiation and infectivity of the parasitic protozoon Leishmania major . To study lysosomal biogenesis, an L. major mutant deficient in the δ subunit of the adaptor...

...Eric Prina; Sofiane Zaki Abdi; Maï Lebastard; Emmanuelle Perret; Nathalie Winter; Jean-Claude Antoine In their mammalian hosts, Leishmania are obligate intracellular parasites that mainly reside in macrophages. They are also phagocytosed by dendritic cells (DCs), which play decisive roles...

...Nathalie Courret; Claude Fréhel; Nelly Gouhier; Marcel Pouchelet; Eric Prina; Pascal Roux; Jean-Claude Antoine Protozoan parasites Leishmania alternate between a flagellated promastigote form and an amastigote form. In their mammalian hosts, Leishmania survive and multiply in macrophages. Both...

...Manika Das; Sikha Bettina Mukherjee; Chandrima Shaha Leishmania donovani promastigotes introduced into the bloodstream by sandfly vectors, are exposed to reactive oxygen species like H 2 O 2 during phagocytosis by the host macrophages. H 2 O 2 can induce promastigote death, but the mechanism...

...Darren R. Brooks; Laurence Tetley; Graham H. Coombs; Jeremy C. Mottram ABSTRACT Removal of the pro-domain of a cysteine protease is essential for activation of the enzyme. We have engineered a cysteine protease (CPB2.8) of the protozoan parasite Leishmania mexicana by site-directed mutagenesis...

... Leishmania Membrane fusion Intracellular pathogens internalized by phagocytosis are sequestered in compartments originating from the plasma membrane, the phagosomes. Newly formed phagosomes are unable to kill and degrade their content and must therefore engage in a complex process of maturation...

...Armelle Cuvillier; Fabienne Redon; Jean-Claude Antoine; Pierre Chardin; Theo DeVos; Gilles Merlin ABSTRACT The small G protein-encoding LdARL-3A gene, a homologue of the human ARL-3 gene, was isolated from Leishmania donovani , and its protein product characterised. It is unique in the Leishmania...

...John A. Maga; Trevor Sherwin; Susan Francis; Keith Gull; Jonathan H. LeBowitz ABSTRACT The paraflagellar rod (PFR) is a unique network of cytoskeletal filaments that lies alongside the axoneme in the flagella of most trypanosomatids. While little is known about how two major Leishmania mexicana PFR...

...Susan Searle; Maria V. McCrossan; Deborah F. Smith ABSTRACT The DNA sequence has been determined of a gene from Leishmania major that shares sequence identity with members of the eukaryotic heat shock protein (hsp) 70 gene family. The deduced open reading frame for translation shares a number...