... at intracellular compartments termed virus-containing compartments (VCCs). Our previous research in HeLa cells – in which assembly resembles that found in infected T cells – suggested that late endosomes/lysosomes (LELs) play a role in HIV-1 trafficking towards its assembly sites. However, the role of LELs during...

... lysosomal function and the balance of protein synthesis and degradation (proteostasis). Here, we treated mammalian cells with the small dipeptide LLOMe, which is known to permeabilize lysosomal membranes, and find that LLOMe also impacts late endosomes (LEs) by neutralizing their pH without causing membrane...

... of organelles. Budding Early endosome Fission Late endosome Recycling endosome Sorting endosome National Institutes of General Medical Sciences 1R01GM123557-01A1 The early endosome (EE), also known as the sorting endosome (SE) (hereafter EE), is the initial destination...

... leads to a highly selective reduction in the cellular levels of a v-SNARE (Vamp8). Moreover, proteomics and immunofluorescence indicate that Vamp8 is associated with Rab17 at late endosomes. Reduced levels of Vamp8 promote transition between ductal carcinoma in situ (DCIS) and a more invasive phenotype...

... et al., 1997 ; Reddy et al., 2001 ), which results in the release of acid hydrolases into the extracellular space and insertion of late endosomal and/or lysosomal membrane proteins into the plasma membrane. Secreted acid hydrolases are thought to degrade the extracellular matrix, facilitating cell...

... cancer by acting in concert with Rab25 to regulate the recycling of α5β1 integrin from late endosomes to the plasma membrane. Here, we show that in two estrogen receptor (ER)-negative breast cancer cell lines, CLIC3 has little influence on integrin recycling, but controls trafficking of the pro-invasive...

... Station [grant number HATCH ALA021-1-09017 to M.C.W.]. Deposited in PMC for release after 12 months. References Aniento   F. , Emans   N. , Griffiths   G. , Gruenberg   J. ( 1993 ). Cytoplasmic dynein-dependent vesicular transport from early to late endosomes.   J. Cell Biol.   123...

... temporally upregulate their secretion of Flii. Using siRNA, and wild-type and mutant proteins, we show that Flii is secreted by means of a late endosomal/lysosomal pathway that is regulated by Rab7 and Stx11. Flii contains 11 leucine-rich repeat domains in its N-terminus that have nearly 50% similarity...

... fluorescent protein in CK1-deficient cells, was detected in dots adjacent to the vacuole. These dots probably correspond to the late endosome/prevacuolar compartment because they were partially colocalized with the Pep12p marker. This accumulation was abolished by mutations affecting the adaptor-related...

... and electron microscopy defines these compartments more precisely as electron-lucent late endosomal and electrondense lysosomal compartments, with Nramp1 colocalizing with Lamp1 and cathepsins D and L in both compartments, with macrosialin in late endosomes, and with BSA-5 nm gold in pre-loaded lysosomes...

... (the light Percoll fraction), late endosomes (the dense Percoll fraction) and lysosomes (the gold fraction). We observed that the proteins linked to the ingested beads are initially cleaved in the phagosomes. This cleavage is inhibited by leupeptin, a thiol-protease inhibitor, and requires an acidic...

...Stéphane Méresse; Jean-Pierre Gorvel; Philippe Chavrier ABSTRACT Rab GTPases belong to the Ras GTPase superfamily and are key regulators of membrane traffic. Among them, rab7 has been localized on late endosomes of NRK cells but its function remains unknown. In order to investigate its role, we...