...Erin G. Harper; Stacy M. Alvares; William G. Carter Quiescent epidermis anchors to laminin 5 in the basement membrane via integrin α6β4. Wounding elevates expression of laminin 5, generating leading keratinocytes (LKs) that migrate via β1 integrins. Laminin 5 was evaluated as a regulator of cell...

...Randy O. Sigle; Susana G. Gil; Mallar Bhattacharya; Maureen C. Ryan; Tai-Mei Yang; Tod A. Brown; Ariel Boutaud; Yuko Miyashita; John Olerud; William G. Carter In epidermal wounds, precursor laminin 5 (α3β3γ2) is deposited in the provisional basement membrane (PBM) before other BM components...

...Miechia A. Esco; Zhiyu Wang; Mark L. McDermott; Michelle Kurpakus-Wheater Laminin 5 functions to promote cell-matrix adhesion and therefore is hypothesized to abrogate apoptosis initiated through the loss of epithelial cell contact with extracellular matrix. Laminin 5 levels are decreased...

... of the corresponding cellular integrins. In this study, we attempt to understand the role of laminin 5 in migration by investigating the integrin-mediated interactions of migrating keratinocytes with their newly synthesized laminin 5. We chose to induce migration of freshly isolated NHK in vitro by exposing them...

... progression. This biological activity was purified and characterized as a laminin 5 (LN5) -related protein. This SFL/LN5 protein consists of the α 3, β3 and γ2 chains of expected sizes. Laminin 5 is a multifunctional secreted glycoprotein thought to be involved in cell adhesion and migration, mainly via its...

... be regulated from within the cell. Glass coverslips were coated overnight with 10 μg/ml human fibronectin (Sigma Chemical Co., St Louis, MO) or 20 μg/ml collagen IV, a gift from Dr E. Engvall (La Jolla Cancer Foundation, La Jolla, CA), in PBS. Laminin 5 matrices were prepared as described previously (Delwel...

...Carien M. Niessen; Esther H. M. Hulsman; Lauran C. J. M. Oomen; I. Kuikman; Arnoud Sonnenberg ABSTRACT The integrin α6β4 is a major component of hemidesmosomes, in which it mediates firm adhesion to laminin 5. Previous studies have shown that the incorporation of α6β4 into hemidesmosomes requires...