... to laminin α5-containing basement membrane matrix, which is produced by luminal cells, presents such a control mechanism. Adhesion to laminin α5 directs basal cells towards a luminal cell fate, and thereby results in a marked decrease of basal MEC progenitor activity in vitro and in vivo . Mechanistically...

... and homeostasis of healthy tissue remains poorly understood. Here, we demonstrate that the Δ133p53 isoforms, the three short isoforms of p53, respond specifically to laminin-111 and play an important regulatory role in formation of mammary organoids in concert with p53α. We demonstrate that down-modulation...

...Hao Xu; Kevin Pumiglia; Susan E. LaFlamme ABSTRACT During angiogenesis, endothelial cells engage components of the extracellular matrix through integrin-mediated adhesion. Endothelial expression of laminin-411 and laminin-511 is known to promote vessel stability. However, little is known about...

...-251 and E-98 glioma cells on reconstituted basement membrane; however, invasion into primary brain slices or 3D astrocyte-based scaffolds and migration on astrocyte-deposited matrix was only partly inhibited. Any residual invasion was supported by vascular structures, as well as laminin 511, a central...

...Philip T. T. Ly; Craig Stewart; Catherine J. Pallen ABSTRACT Extrinsic signals that regulate oligodendrocyte maturation and subsequent myelination are essential for central nervous system development and regeneration. Deficiency in the extracellular factor laminin-2 (Lm2, comprising the α2β1γ1...

... to their affinity states. Laminin Integrin Electron microscopy Conformational change Ministry of Education, Culture, Sports, Science and Technology 10.13039/501100001700 JP17H01420 Japan Agency for Medical Research and Development 10.13039/100009619 JP17am0101075...

... prostatic intraepithelial neoplasia (HG-PIN) tissue has enlarged nuclei and nucleoli, luminal layer expansion and genomic instability. Additional HG-PIN markers include loss of α6β4 integrin or its ligand laminin-332, and budding of tumor clusters into laminin-511-rich stroma. We modeled the invasive...

... contributes to the progression of a wide range of human diseases, including cancers. Here, we reveal the pathway and mechanism for the endocytic trafficking of a prominent basement membrane protein, laminin-111 (referred to here as laminin), and their disruption in disease. Live-cell imaging of epithelial...

... Laminin Integrin ILK Polarity Microtubule +TIPS Endocytosis Polarised epithelial cells form a continuous layer in which cells are connected by tight and adherens junctions, creating a barrier that separates the inside of our bodies from the outside environment. In many tissues, the apical...

... by phosphorylating Par-3. In addition, Par-1 has been shown to impact on cell–ECM interactions by regulating laminin receptor localization and laminin deposition on the basal surface of epithelial cells. Laminins are major structural and signaling components of basement membrane (BM), a sheet of specialized ECM...

...Xiuwei H. Yang; Rossen Mirchev; Xinyu Deng; Patrick Yacono; Helen L. Yang; David E. Golan; Martin E. Hemler Laminin-binding integrins (α3β1, α6β1, α6β4, α7β1) are almost always expressed together with tetraspanin CD151. In every coexpressing cell analyzed to date, CD151 makes a fundamental...

... in regulating the interaction of breast cancer cells with the ECM. Using epithelial cells from breast tumors, we defined NRP2 high and NRP2 low populations that differed in integrin expression and adhesion to laminin. Specifically, the NRP2 high population adhered more avidly to laminin and expressed high...

...Daniela Urich; Jessica L. Eisenberg; Kevin J. Hamill; Desire Takawira; Sergio E. Chiarella; Saul Soberanes; Angel Gonzalez; Frank Koentgen; Tomas Manghi; Susan B. Hopkinson; Alexander V. Misharin; Harris Perlman; Gokhan M. Mutlu; G. R. Scott Budinger; Jonathan C. R. Jones Laminins...

...Virginia A. Spencer; Sylvain Costes; Jamie L. Inman; Ren Xu; James Chen; Michael J. Hendzel; Mina J. Bissell Functional differentiation is orchestrated by precise growth-regulatory controls conveyed by the tissue microenvironment. Cues from laminin 111 (LN1) lower transcription and suppress mammary...

... formation, even though cultured Dag1 -null epithelial cells failed to assemble laminin-111 at the cell surface. The absence of DG was, however, associated with a marked loss in activity of signal transducer and activator of transcription 5 (STAT5). Loss of DG perturbed STAT5 signaling induced by either...

... the potential role of extracellular signals in INM and planar divisions using the medaka mutant tacobo ( tab ). This tab mutant shows pleiotropic phenotypes, including neurogenesis, and positional cloning identified tab as laminin γ 1 ( lamc1 ), providing a unique framework to study the role of extracelluar...

... pathways that control either proliferation or morphogenesis, and laminin is essential for SC proliferation. However, it is not clear whether laminin is also required for SC morphogenesis. By using a novel time-lapse live-cell-imaging technique, we demonstrated that laminins are required for SCs to form...

...Naoki Ichikawa; Kazuhisa Iwabuchi; Hidetake Kurihara; Kumiko Ishii; Toshihide Kobayashi; Takako Sasaki; Nobutaka Hattori; Yoshikuni Mizuno; Kentaro Hozumi; Yoshihiko Yamada; Eri Arikawa-Hirasawa Laminin-1, an extracellular matrix molecule, promotes neurite outgrowth through the interaction...

... requires p38 and activation of NFκB requires IκB kinase-β. Activation of β-catenin, important in Wnt signaling and the cause of most colon cancers, is inhibited by shear stress through a pathway involving laminin-5, α6β4 integrin, phosphoinositide 3-kinase (PI 3-kinase) and Rac1 coupled with changes...

...Christi A. Weston; Getu Teressa; Benjamin S. Weeks; Joav Prives During neuromuscular junction formation, extracellular matrix-mediated signals cause muscle surface acetylcholine receptors (AChRs) to aggregate at synaptic sites. Two extracellular matrix proteins, agrin and laminin, have each been...