... in the presence of dynamic F-actin exhibit a striking bilobed nuclear morphology with distinct membrane compositions in the two lobes and accumulation of F-actin at the inner nuclear envelope. The addition of Lamin A (encoded by lmna), which is absent from Xenopus eggs, results in rounder nuclei, suggesting...

...Sandra Vidak; Konstantina Georgiou; Petra Fichtinger; Nana Naetar; Thomas Dechat; Roland Foisner ABSTRACT A-type lamins are components of the peripheral nuclear lamina but also localize in the nuclear interior in a complex with lamina-associated polypeptide (LAP) 2α. Loss of LAP2α and nucleoplasmic...

...Vitaly Kochin; Takeshi Shimi; Elin Torvaldson; Stephen A. Adam; Anne Goldman; Chan-Gi Pack; Johanna Melo-Cardenas; Susumu Y. Imanishi; Robert D. Goldman; John E. Eriksson ABSTRACT Nuclear lamins form the major structural elements that comprise the nuclear lamina. Loss of nuclear structural...

... of the nuclear envelope protein SUN1 contributes to HGPS nuclear aberrancies. However, the mechanism by which interactions between mutant lamin A (also known as progerin or LAΔ50) and SUN1 produce HGPS cellular phenotypes requires further elucidation. Using light and electron microscopy, this study demonstrated...

... in human cells showed that barrier-to-autointegration factor (BAF), a DNA-binding protein, assembled first at the distinct `core' region of the telophase chromosome and formed an immobile complex by directly binding with other core-localizing NE proteins, such as lamin A and emerin. Correlative light...

...Martin W. Goldberg; Irm Huttenlauch; Christopher J. Hutchison; Reimer Stick Lamins are intermediate filament proteins and the major component of the nuclear lamina. Current views of the lamina are based on the remarkably regular arrangement of lamin LIII in amphibian oocyte nuclei. We have re...

... 30% (asynchronous controls) to ∼80%. In HeLa cells, RNAi-induced downregulation of BAF significantly increased the proportion of early S-phase cells that retained high levels of cyclin D3 and cyclin E expression and slowed progression through early S phase. BAF downregulation also caused lamin...

...Yiyong Liu; Antonio Rusinol; Michael Sinensky; Youjie Wang; Yue Zou The genetic diseases Hutchinson-Gilford progeria syndrome (HGPS) and restrictive dermopathy (RD) arise from accumulation of farnesylated prelamin A because of defects in the lamin A maturation pathway. Both of these diseases...

...Michael S. Zastrow; Denise B. Flaherty; Guy M. Benian; Katherine L. Wilson Lamins form structural filaments in the nucleus. Mutations in A-type lamins cause muscular dystrophy, cardiomyopathy and other diseases, including progeroid syndromes. To identify new binding partners for lamin A, we carried...

...Jens Gruber; Tina Lampe; Mary Osborn; Klaus Weber FACE 1 is the endoprotease responsible for cleavage of prelamin A to lamin A. Transfection of HeLa cells with siRNA for human FACE 1 results in a strong phenotype. Protein and mRNA levels for FACE 1 are knocked down and cell division stops abruptly...

...Kenneth K. Lee; Tokuko Haraguchi; Richard S. Lee; Takako Koujin; Yasushi Hiraoka; Katherine L. Wilson Loss of emerin, a lamin-binding nuclear membrane protein, causes Emery-Dreifuss muscular dystrophy. We analyzed 13 site-directed mutations, and four disease-causing mutations that do not disrupt...

... not assemble into the NE during the subsequent interphase. BAF mutant G25E also dominantly dislocalized LAP2β and lamin A from the NE, but had no effect on the localization of lamin B. We conclude that BAF is required for the assembly of emerin and A-type lamins at the reforming NE during telophase, and may...

...Bh. Muralikrishna; Jyotsna Dhawan; Nandini Rangaraj; Veena K. Parnaik Intranuclear lamin foci or speckles have been observed in various cell types. In order to explore the possibility of changes in internal lamin organization during muscle differentiation, we have examined the appearance of A-type...

...M. Sinensky; T. McLain; K. Fantle ABSTRACT The role of inhibition of prelamin A processing in the inhibition of DNA synthesis by lovastatin was examined by expressing prelamin A in F9 teratocarcinoma cells. These cells, normally lacking expression of the A/C lamins, were transfected with constructs...

...Hartwig Hennekes; Erich A. Nigg ABSTRACT Mature A- and B-type lamins differ in the extent to which they interact with the nuclear membrane and thus represent an interesting model for studying the role of isoprenylation and carboxyl-methylation in membrane attachment. Both A- and B-type lamins...

...M. Sinensky; K. Fantle; M. Trujillo; T. McLain; A. Kupfer; M. Dalton ABSTRACT The conversion of mammalian prelamin A to mature lamin A proceeds through the removal of 18 amino acids from the carboxyl terminus. The initial step in this processing is the isoprenylation of a CAAX box cysteine...