... chromosomal rearrangements and DNA damage. Nuclear blebs (i.e. herniations of the nuclear envelope) can be induced by (1) nuclear compression, (2) nuclear migration (e.g. cancer metastasis), (3) actin contraction, (4) lamin mutation or depletion, and (5) heterochromatin enzyme inhibition. Recent work has...

...Jacob Odell; Jan Lammerding ABSTRACT Lamins are intermediate filament proteins that contribute to numerous cellular functions, including nuclear morphology and mechanical stability. The N-terminal head domain of lamin is crucial for higher order filament assembly and function, yet the effects...

...Takanobu Moriuchi; Fumiko Hirose ABSTRACT Dephosphorylation of lamin A, which triggers nuclear lamina reconstitution, is crucial for the completion of mitosis. However, the specific phosphatase and regulatory mechanism that allow timely lamin A dephosphorylation remain unclear. Here, we report...

...Chayki Charar; Sally Metsuyanim-Cohen; Daniel Z. Bar ABSTRACT Animals subjected to dietary restriction (DR) have reduced body size, low fecundity, slower development, lower fat content and longer life span. We identified lamin as a regulator of multiple dietary restriction phenotypes...

... analysis. We then examined which of these proteins are required for the NE localization of PIG-B. Knockdown of Lamin Dm0, a B-type lamin, led to mislocalization of PIG-B from the NE to the endoplasmic reticulum. Lamin Dm0 associated with PIG-B at the inner nuclear membrane, a process that required the tail...

.... However, the molecular mechanisms that regulate nuclear structure–function relationships during heat shock remain unclear. Here, we show that lamin A and C (hereafter lamin A/C; both lamin A and C are encoded by LMNA) are required for heat-shock-mediated transcriptional induction of the Hsp70 gene locus...

... and heterochromatin, and have active cell cycles. We found that nuclear branches are disrupted by loss of filamentous actin and depend on epidermal expression of the nuclear lamina protein Lamin B1. Inhibition of nuclear branching disrupts fin morphology, suggesting that nuclear branching may be involved in fin...

... chromosomes (for a full review on nuclear envelope breakdown see Güttinger et al., 2009). This process includes the dissociation of the nuclear envelope and NPCs from lamins and chromatin (Güttinger et al., 2009). NPCs have a primary role in the mitotic process. Through selective transport, nuclear pores...

...Gun West; Josef Gullmets; Laura Virtanen; Song-Ping Li; Anni Keinänen; Takeshi Shimi; Monika Mauermann; Tiina Heliö; Maija Kaartinen; Laura Ollila; Johanna Kuusisto; John E. Eriksson; Robert D. Goldman; Harald Herrmann; Pekka Taimen ABSTRACT Mutation of the LMNA gene, encoding nuclear lamin...

... at the endoproteolytic cleavage site in chicken prelamin A abolishes its conversion to lamin A. The consequences of this mutation in an organism have remained unknown. We now report that the corresponding mutation in a human subject leads to accumulation of prelamin A and causes a progeroid disorder. Next generation...

... that the recombination mediator Brca2 associates with lamin and the cohesion factor Pds5 to secure persistent recombination sites at the nuclear envelope. In Rad51 −/− females, all persistent DNA breaks are directed to the nuclear envelope. By contrast, a reduction of Pds5 or Brca2 levels abolishes the movement and has...

... arrays revealed direct binding of residues 170–220 to residues 206–225 (the proposed positive element), residues 147–174 (particularly P 153 MYGRDSAYQSITHYRP 169) and the LEM domain. Emerin residues 1–132 and 159–220 were each sufficient to bind lamin A or B1 tails in vitro , identifying two independent...

... lamins than in cells from wild-type mice. Knockdown of Sun2 also increased the mobility of a short, chimeric form of nesprin-2 giant (mini-nesprin-2G), whereas the lack of emerin did not affect the mobility of Sun1, Sun2 or mini-nesprin-2G. Fluorescence resonance energy transfer experiments showed Sun1...

... instability during NEBD. The function of Lpin-1 reported here is probably unique to metazoa, which produce Lamin filaments to stabilize the NE; these filaments must be depolymerized during NEBD. All worm strains were cultured using standard methods at 20°C. The following strains were used: GFP...

... was that the barrier is a membranous structure. However, the abundance of ER membrane around spindles of normally dividing cells made it difficult to determine unequivocally whether there was an enrichment of ER membrane between the two parallel spindles. Therefore, we used the C. elegans lamin protein, LMN-1, fused...

... to the lysosome via retrograde membrane trafficking. Nuclear envelope localization of proAR involves truncation of the C-terminus, which subsequently activates the ER-retrieval signal. The truncated form of proAR interacts with A-type lamin and is retained at the inner nuclear membrane. Heterochromatin formation...

...Xiaomin Bao; Weiguo Zhang; Robert Krencik; Huai Deng; Yanming Wang; Jack Girton; Jørgen Johansen; Kristen M. Johansen We have used a yeast two-hybrid screen to identify lamin Dm 0 as an interaction partner for the nuclear JIL-1 kinase. This molecular interaction was confirmed by GST-fusion protein...

... transmembrane domain and/or to N-terminal paired calponin homology (CH) domains. The smaller isoforms of nesprin-2 co-localize with and bind lamin A and emerin at the inner nuclear envelope (NE). In SW-13 cells, which lack lamin A/C, nesprin-2 epitopes and emerin were both mislocalized and formed aggregates...

...Kristina Prüfert; Annette Vogel; Georg Krohne We analyzed the influence of lamins on nuclear envelope growth in cultured Xenopus A6 cells by the overexpression of human lamin A, Xenopus and zebrafish lamins B2 and Drosophila lamins Dm0 and C as GFP fusion proteins. Lamins containing a CxxM motif...

.... At the light microscopic level, grossly aberrant nuclear lamina structure and B-type lamin distribution correlated well with the loss of detectable amounts of BAF protein from nuclei. Together, these data represent evidence of BAF's anticipated function in mediating interactions between the nuclear envelope...