... morphologically distinct precursor types – growth cone splitting (blue), filopodium (red), lamellipodium/actin wave (green) and a mixed type (yellow). Long-term live-cell recordings were undertaken with a Nikon Eclipse Ti microscope equipped with a small stagetop as well as full incubator enclosure...

... and their subsequent stabilization by nascent adhesions. The actin-binding protein lamellipodin (Lpd) is thought to play a critical role in lamellipodium protrusion, by delivering Ena/VASP proteins onto the growing plus ends of actin filaments and by interacting with the WAVE regulatory complex, an activator...

...://www.singerlab.org/protocols on H1299 wild-type cells or stable lines expressing the GFP–VICKZ constructs with the following modifications. To induce lamellipodium formation, cells were starved overnight in complete medium without FCS, followed by addition of complete medium containing 10% FCS for 30 minutes. 20 ng...

... polymerisation of branched actin networks. In order to identify new SWC regulators, we conducted a screen in Drosophila cells combining proteomics with functional genomics. This screen identified Clathrin heavy chain (CHC) as a protein that binds to the SWC and whose depletion affects lamellipodium formation...

... for correspondence (e-mail: [email protected] ) 24 10 2005 © The Company of Biologists Limited 2006 2006 GTPase-activating protein ARAP3 PI3K Rho Arf Lamellipodium Many cells respond to appropriate signals by forming lamellipodia, dynamic, sheet-like membrane protrusions...

... to direct the correct spatial activation of membrane protrusion. * Author for correspondence (e-mail: [email protected] ) 16 3 2005 © The Company of Biologists Limited 2005 2005 FAK Cell polarity Cell migration Lamellipodium The migration of cells is essential for a wide...

... 3 2004 © The Company of Biologists Limited 2004 2004 Actin-bundling activity Actin-stabilizing activity Caldesmon Focal adhesion Wound-healing assay Lamellipodium Calmodulin (CaM) plays a crucial role in the regulation of cell motility ( Chin and Means, 2000 ). An increase...

... adhesions Actin Vinculin Lamellipodium Cell migration is important to many crucial biological functions such as embryogenesis, immune responses, wound healing and cancer metastasis ( Stossel, 1993 ). The process involves a number of well-orchestrated steps including protrusion, adhesion...

... architecture. © The Company of Biologists Limited 2002 2002 Actin Cytoskeleton Lamellipodium Motility Cryo-electron microscopy Frozen hydrated samples were examined with a Philips CM 200 FEG at 200 kV in TEM low-dose bright-field mode. A GATAN 626 cryostage cooled to approximately 90 K...

... to form lamellipodia, in part, by regulating its trafficking to the plasma membrane. ARF6 Rac Rho Actin Lamellipodium AlF treatment induces actin rearrangements in both Rac1- and ARF6-transfected cells, but the nature of the rearrangements varies (ruffles or protrusions) depending upon...

... locomotion. Therefore, to reconstitute contact inhibition completely it may be necessary to add both an inducer of the turning reaction (such as the membrane preparations we used here) and a physical barrier that obstructs cell movement in the original direction. Although an extensive lamellipodium...