... family. Two such proteins, Pil1 and Lsp1, participate in eisosome formation in the yeast Saccharomyces cerevisiae . Under normal laboratory conditions, deletion of the PIL1 gene results in the inability of cells to assemble wild-type-like eisosomes. We found that under certain stress conditions, Lsp1...

... antibody (pAb) (1:300) was purchased from Sigma-Aldrich. Rabbit-anti-LSP1 pAb (1:100) was purchased from Atlas Antibodies (Bromma, Sweden). Mouse anti-vinculin monoclonal antibody (mAb) (1:200) was purchased from Sigma-Aldrich. Fluorochrome-coupled secondary antibodies (all conjugated with either...

... 1 (LSP1) binds to PKCβI and expression of B-LSP1, an LSP1 truncate containing the PKCβI binding residues, inhibits anti-IgM-induced translocation of PKCβI to the plasma membrane and anti-IgM-induced activation of ERK2. To understand the role of LSP1 in the regulation of PKCβI-dependent ERK2...

...V. L. Misener; C.-c. Hui; I. A. Malapitan; M.-E. Ittel; A. L. Joyner; J. Jongstra ABSTRACT Mouse LSP1 is a 330 amino acid intracellular F-actin binding protein expressed in lymphocytes and macrophages but not in non-hematopoietic tissues. A 328 amino acid LSP1-related protein, designated S37...