.... , Birgisdottir , A. B. , Larsen , K. B. , Jain , A. , Olsvik , H. , Øvervatn , A. , Kirkin , V. and Johansen , T. ( 2012 ). ATG8 family proteins act as scaffolds for assembly of the ULK complex: sequence requirements for LC3-interacting region (LIR) motifs . J. Biol. Chem. 287...

... work is properly attributed. Summary: The role of the core ATG8 system during autophagosome formation and autophagosome-lysosome fusion. ATG8 Autophagosome Autophagy GABARAP LC3 National Health and Medical Research Council http://dx.doi.org/10.13039/501100000925...

... by The Company of Biologists Ltd 2020 Summary: Foot-and-mouth disease virus infection induces ER stress and the PERK-mediated UPR, which activates autophagic flux to promote virus multiplication by affecting antiviral interferon production. Autophagy Foot-and-mouth disease virus Interferon LC3...

...-1/ Summary: The necroptotic effector MLKL can inhibit the function of autolysosomes, thereby reducing autophagic flux. Necroptosis Autophagy MLKL LC3 Macroautophagy (hereafter called autophagy) is a lysosomal degradation and recycling process of cellular components...

... of the trans -Golgi network, GCC88 (encoded by GCC1 ), regulates the balance between Golgi mini-stacks and the Golgi ribbon. Loss of Golgi ribbons in stable cells overexpressing GCC88 resulted in compromised mechanistic target of rapamycin (mTOR) signaling and a dramatic increase in LC3-II-positive...

... and biogenesis. In higher eukaryotes, the Atg8 family comprises six members known as the light chain 3 (LC3) or γ-aminobutyric acid (GABA)-receptor-associated protein (GABARAP) proteins. Considerable effort during the last 15 years to decipher the molecular mechanisms that govern autophagy has significantly...

... of this selectivity lies the LC3-interacting region (LIR) motif, which ensures the targeting of autophagy receptors to LC3 (or other ATG8 family proteins) anchored in the phagophore membrane. LIR-containing proteins include cargo receptors, members of the basal autophagy apparatus, proteins associated with vesicles...

... evidence that post-Golgi membrane traffic plays a crucial role in autophagosome formation. Increased secretion of constitutive cargo from the trans-Golgi network (TGN) to the plasma membrane induced the formation of microtubule-associated protein light chain 3 (LC3)-positive structures. At the early phase...

... the autophagy machinery is involved in this specific type of autophagy. It has been speculated that adaptor proteins such as p62 might mediate the interaction between the autophagosomal LC3 family of proteins and ubiquitylated proteins on mitochondria. Here, we describe our systematic analysis...

... for the treatment of pathological conditions in which manipulation of autophagic flux is thought to be beneficial. ‡ Author for correspondence ( [email protected] ) * These authors contributed equally to this work 30 6 2009 © The Company of Biologists Limited 2009 2009 LC3 Cell death...

... The Company of Biologists Limited 2009 2009 Autophagy Autophagin Atg4 Atg8 LC3 GABARAP-L1 Macroautophagy (autophagy, hereafter) is an important catabolic process that promotes cell survival during episodes of nutrient or growth-factor stress ( Lum et al., 2005 ). Autophagy has important...

... chain 3 (LC3) of microtubule-associated proteins 1A and 1B. Since human fibrosarcoma (HT1080) cells produce little fibronectin and LC3, we used these cells to investigate how LC3-mediated Fn1 mRNA translation might alter tumor growth. Transfection of HT1080 cells with LC3 enhanced fibronectin mRNA...

...Elena Shvets; Ephraim Fass; Ruthie Scherz-Shouval; Zvulun Elazar LC3 belongs to a novel ubiquitin-like protein family that is involved in different intracellular trafficking processes, including autophagy. All members of this family share a unique three-dimensional structure composed of a C...

... this by inhibiting Rab7 function with RNA interference and overexpression of dominant negative Rab7. We show here that Rab7 was needed for the formation of preferably perinuclear, large aggregates, where the autophagosome marker LC3 colocalised with Rab7 and late endosomal and lysosomal markers. By electron...

.... Furthermore, a marked increase in the size of the monodansylcadaverine-labeled vacuoles induced by starvation was observed by overexpression of the inactive mutant T22N. Similarly, there was an increase in the size of vesicles labeled with LC3, a protein that specifically localizes on the autophagosomal...

... of autophagosomes is upregulated by amino acid deprivation. Furthermore, these MDC-vacuoles were labeled with LC3, one of the mammalian homologues of the yeast protein Apg8/Aut7 that plays an important role in autophagosome formation. Autophagy was induced by amino acid starvation. Cells were washed three times...