...Ana C. Almeida; Helder Rocha; Maximilian W. D. Raas; Hanh Witte; Ralf J. Sommer; Berend Snel; Geert J. P. L. Kops; Reto Gassmann; Helder Maiato ABSTRACT Chromosome alignment during mitosis can occur as a consequence of bi-orientation or is assisted by the CENP-E (kinesin-7) motor at kinetochores...

...Anna Gavrilova; Astrid Boström; Nickolay Korabel; Sergei Fedotov; Gino B. Poulin; Victoria J. Allan ABSTRACT Fast axonal transport is crucial for neuronal function and is driven by kinesins and cytoplasmic dynein. Here, we investigated the role of kinesin-1 in dense core vesicle (DCV) transport...

... tracks. Dynein and kinesins govern autophagosome trafficking on microtubules through the sequential recruitment of their effector proteins, post-translational modifications and interactions with LC3-interacting regions (LIRs). In contrast, myosins are actin-based motors that participate in various stages...

...Kristen J. Verhey; Ryoma Ohi ABSTRACT Microtubules are critical for a variety of important functions in eukaryotic cells. During intracellular trafficking, molecular motor proteins of the kinesin superfamily drive the transport of cellular cargoes by stepping processively along the microtubule...

... of LE/Ls. A proteomic survey of purified LE/Ls identified StARD9 as a novel lysosomal kinesin responsible for LE/L tubulation. StARD9 contains an N-terminal kinesin domain, a C-terminal StART domain, and a dileucine signal shared with other lysosome-associated membrane proteins. Depletion of StARD9...

... kinesin-14 family members, but not dynein, inhibits CD166 endocytosis, indicating that a tight coordination between CD166 endosome formation, cortex tension and MT association regulates this process ( Tyckaert et al., 2022 ). MHC-I and CD59 endocytosis Myosin-II has also been identified as regulating...

...Kyoko Chiba; Tomoki Kita; Yuzu Anazawa; Shinsuke Niwa ABSTRACT Neuronal function depends on axonal transport by kinesin superfamily proteins (KIFs). KIF1A is the molecular motor that transports synaptic vesicle precursors, synaptic vesicles, dense core vesicles and active zone precursors. KIF1A...

... endosomal choreography, which includes bidirectional and stop-and-go motions, is coordinated by the microtubule-based motor proteins dynein and kinesin. These motors bridge various endosomal subtypes to the microtubule tracks thanks to their cargo-binding domain interacting with endosome-associated proteins...

..., for intracellular transport. Here we describe how molecular motors and cytoskeletal tracks can generate streaming. Actin Cytoplasmic streaming Dynein Kinesin Microtubules Myosin National Institute of General Medical Sciences http://dx.doi.org/10.13039/100000057 R35 GM131752...

... of microtubules. The central pair of microtubules are surrounded by a set of proteins, termed the central pair apparatus. A specific kinesin, Klp1 projects from the central pair and contributes to ciliary motility in Chlamydomonas . The vertebrate ortholog, Kif9, is required for beating in mouse sperm flagella...

...Toni McHugh; Julie P. I. Welburn ABSTRACT The precise regulation of microtubule length during mitosis is essential to assemble and position the mitotic spindle and segregate chromosomes. The kinesin-13 Kif2C or MCAK acts as a potent microtubule depolymerase that diffuses short distances...

...Jessica J. A. Hummel; Casper C. Hoogenraad ABSTRACT Molecular motors drive long-range intracellular transport of various vesicles and other cargoes within a cell. Identifying which kinesin motors interact with which type of transport vesicles has been challenging, especially in complex neuronal...

...T. Lynne Blasius; Yang Yue; RaghuRam Prasad; Xinglei Liu; Arne Gennerich; Kristen J. Verhey ABSTRACT The kinesin-4 member KIF7 plays critical roles in Hedgehog signaling in vertebrate cells. KIF7 is an atypical kinesin as it binds to microtubules but is immotile. We demonstrate that, like...

... in building the cilium by shuttling structural components and signaling receptors between the ciliary base and tip. To provide effective transport, IFT-A and IFT-B adaptor protein complexes assemble into highly repetitive polymers, called IFT trains, that are powered by the motors kinesin-2 and IFT-dynein...

... of success exiting the branch based on orientation: correctly oriented minus-end-out microtubules succeeded in leaving about twice as often as incorrectly oriented microtubules. Increased success relied on other microtubules in a parallel orientation. From a candidate screen, we identified Trim9 and kinesin...

... with mitochondria – the actin-based myosins, as well as the microtubule-based kinesins and dynein. In addition, Miro and TRAK proteins act as adaptors that link kinesin-1 and dynein, as well as myosin of class XIX (MYO19), to mitochondria and coordinate microtubule- and actin-based motor activities. Here, we...

...Gina A. Monzon; Lara Scharrel; Ashwin DSouza; Verena Henrichs; Ludger Santen; Stefan Diez ABSTRACT The maintenance of intracellular processes, like organelle transport and cell division, depend on bidirectional movement along microtubules. These processes typically require kinesin and dynein motor...

... eukaryotes, the intraflagellar transport (IFT) machinery drives flagellum biogenesis, and anterograde IFT requires kinesin-2 motor proteins. In this study, we investigated the function of the two T. brucei kinesin-2 proteins, TbKin2a and TbKin2b, in bloodstream form trypanosomes. We found that, compared...

... underwent the typical three phases of spindle elongation, they have distinct features. We found that the relative concentration of the kinesin-14 family protein Pkl1 is decreased in meiosis I compared to mitosis, while the concentration of the kinesin-5 family protein Cut7 remains constant. We identified...

... and germ cell tumours. The kinesin KIF18A is an important regulator of those processes in animal germ cells. Post-transcriptional regulation of KIF18A has not been extensively explored. Owing to the presence of PUM-binding elements (PBEs), KIF18A mRNA is a potential target of PUM proteins, where PUM refers...