...Kristen J. Verhey; Ryoma Ohi ABSTRACT Microtubules are critical for a variety of important functions in eukaryotic cells. During intracellular trafficking, molecular motor proteins of the kinesin superfamily drive the transport of cellular cargoes by stepping processively along the microtubule...

... and cargo adaptors. Cytoskeleton Membrane trafficking Motor proteins Dynein Kinesin Myosin Cargo adaptors Endocytosis EMBL Australia https://doi.org/10.13039/100016146 Endocytosis controls the uptake of cargoes, such as receptors, nutrients and lipids, which...

... of LE/Ls. A proteomic survey of purified LE/Ls identified StARD9 as a novel lysosomal kinesin responsible for LE/L tubulation. StARD9 contains an N-terminal kinesin domain, a C-terminal StART domain, and a dileucine signal shared with other lysosome-associated membrane proteins. Depletion of StARD9...

...Kyoko Chiba; Tomoki Kita; Yuzu Anazawa; Shinsuke Niwa ABSTRACT Neuronal function depends on axonal transport by kinesin superfamily proteins (KIFs). KIF1A is the molecular motor that transports synaptic vesicle precursors, synaptic vesicles, dense core vesicles and active zone precursors. KIF1A...

... endosomal choreography, which includes bidirectional and stop-and-go motions, is coordinated by the microtubule-based motor proteins dynein and kinesin. These motors bridge various endosomal subtypes to the microtubule tracks thanks to their cargo-binding domain interacting with endosome-associated proteins...

... following streaming-dependent delivery. Actin reorganization, the actin-based motor myosin-V, RNA-binding proteins (such as Osk, Rump and Lost), cortical microtubules and microtubule-based motors (kinesin-1 and dynein) are all implicated in capturing and accumulating specific mRNAs and proteins...

... of microtubules. The central pair of microtubules are surrounded by a set of proteins, termed the central pair apparatus. A specific kinesin, Klp1 projects from the central pair and contributes to ciliary motility in Chlamydomonas . The vertebrate ortholog, Kif9, is required for beating in mouse sperm flagella...

...Toni McHugh; Julie P. I. Welburn ABSTRACT The precise regulation of microtubule length during mitosis is essential to assemble and position the mitotic spindle and segregate chromosomes. The kinesin-13 Kif2C or MCAK acts as a potent microtubule depolymerase that diffuses short distances...

...Jessica J. A. Hummel; Casper C. Hoogenraad ABSTRACT Molecular motors drive long-range intracellular transport of various vesicles and other cargoes within a cell. Identifying which kinesin motors interact with which type of transport vesicles has been challenging, especially in complex neuronal...

...T. Lynne Blasius; Yang Yue; RaghuRam Prasad; Xinglei Liu; Arne Gennerich; Kristen J. Verhey ABSTRACT The kinesin-4 member KIF7 plays critical roles in Hedgehog signaling in vertebrate cells. KIF7 is an atypical kinesin as it binds to microtubules but is immotile. We demonstrate that, like...

... in building the cilium by shuttling structural components and signaling receptors between the ciliary base and tip. To provide effective transport, IFT-A and IFT-B adaptor protein complexes assemble into highly repetitive polymers, called IFT trains, that are powered by the motors kinesin-2 and IFT-dynein...

... of success exiting the branch based on orientation: correctly oriented minus-end-out microtubules succeeded in leaving about twice as often as incorrectly oriented microtubules. Increased success relied on other microtubules in a parallel orientation. From a candidate screen, we identified Trim9 and kinesin...

... with mitochondria – the actin-based myosins, as well as the microtubule-based kinesins and dynein. In addition, Miro and TRAK proteins act as adaptors that link kinesin-1 and dynein, as well as myosin of class XIX (MYO19), to mitochondria and coordinate microtubule- and actin-based motor activities. Here, we...

...Gina A. Monzon; Lara Scharrel; Ashwin DSouza; Verena Henrichs; Ludger Santen; Stefan Diez ABSTRACT The maintenance of intracellular processes, like organelle transport and cell division, depend on bidirectional movement along microtubules. These processes typically require kinesin and dynein motor...

... eukaryotes, the intraflagellar transport (IFT) machinery drives flagellum biogenesis, and anterograde IFT requires kinesin-2 motor proteins. In this study, we investigated the function of the two T. brucei kinesin-2 proteins, TbKin2a and TbKin2b, in bloodstream form trypanosomes. We found that, compared...

... underwent the typical three phases of spindle elongation, they have distinct features. We found that the relative concentration of the kinesin-14 family protein Pkl1 is decreased in meiosis I compared to mitosis, while the concentration of the kinesin-5 family protein Cut7 remains constant. We identified...

... and germ cell tumours. The kinesin KIF18A is an important regulator of those processes in animal germ cells. Post-transcriptional regulation of KIF18A has not been extensively explored. Owing to the presence of PUM-binding elements (PBEs), KIF18A mRNA is a potential target of PUM proteins, where PUM refers...

..., but the mechanistic relationship between the two states is poorly understood. Here, we show that spindle polarity (e.g. bipolarity or multipolarity) in tetraploid cells depends on the level of functional phosphorylated Eg5, a mitotic kinesin, localised to the spindle. Multipolar spindles are formed in cells with high...

...Alison E. Twelvetrees; Flavie Lesept; Erika L. F. Holzbaur; Josef T. Kittler ABSTRACT Binding of motor proteins to cellular cargoes is regulated by adaptor proteins. HAP1 and GRIP1 are kinesin-1 adaptors that have been implicated individually in the transport of vesicular cargoes in the dendrites...

... mutant lysate spent 71.3% of the time growing, 12.1% shrinking and 16.6% pausing, which was quite different from wild-type lysates in anaphase, but not that different from the single kip3Δ mutant ( Fig. 4 D). Based on our observations, we conclude that these two kinesin-family proteins contribute to MT...