...Erez Cohen; Craig Johnson; Catherine J. Redmond; Raji R. Nair; Pierre A. Coulombe ABSTRACT A large group of keratin genes ( n =54 in the human genome) code for intermediate filament (IF)-forming proteins and show differential regulation in epithelial cells and tissues. Keratin expression can...

...Lisa te Molder; Jose M. de Pereda; Arnoud Sonnenberg ABSTRACT Hemidesmosomes (HDs) are specialized multiprotein complexes that connect the keratin cytoskeleton of epithelial cells to the extracellular matrix (ECM). In the skin, these complexes provide stable adhesion of basal keratinocytes...

...Sujin Kim; Younglan Lim; So-Young Lee; Han-Na Yoon; Hayan Yi; Kwi-Hoon Jang; Nam-On Ku ABSTRACT Keratin 8 (K8) is the cytoskeletal intermediate filament protein of simple-type epithelia. Mutations in K8 predispose the affected individual and transgenic mouse to liver disease. However, the role...

...Justin T. Jacob; Raji R. Nair; Brian G. Poll; Christopher M. Pineda; Ryan P. Hobbs; Michael J. Matunis; Pierre A. Coulombe ABSTRACT Keratin 17 ( KRT17 ; K17), a non-lamin intermediate filament protein, was recently found to occur in the nucleus. We report here on K17-dependent differences...

...So-Young Lee; Sujin Kim; Younglan Lim; Han-Na Yoon; Nam-On Ku ABSTRACT Intermediate filament protein keratin 8 (K8) binds to heat shock protein 70 (Hsp70) and p38 MAPK, and is phosphorylated at Ser74 by p38α (MAPK14, hereafter p38). However, a p38 binding site on K8 and the molecular mechanism...

...Han-Na Yoon; Sung-Yeon Yoon; Jin-Hyuck Hong; Nam-On Ku ABSTRACT Cytoskeletal keratin 18 (K18) undergoes caspase-mediated digestion during apoptosis, which leads to dramatic disassembly of keratin filaments. We studied the significance of K18 caspase digestion in a mouse model and generated...

...Stéphane Gilbert; Anne Loranger; M. Bishr Omary; Normand Marceau ABSTRACT Keratins are epithelial cell intermediate filament (IF) proteins that are expressed as pairs in a cell-differentiation-regulated manner. Hepatocytes express the keratin 8 and 18 pair (denoted K8/K18) of IFs, and a loss of K8...

... of the cytoskeleton to nuclear mechanotransduction remains unclear. In this study, we examine how crosstalk between the cytolinker plectin and F-actin controls keratin network organisation and the 3D nuclear morphology of keratinocytes. Using micro-patterned surfaces to precisely manipulate cell shape, we find...

...Catharina M. Alam; Jonas S. G. Silvander; Ebot N. Daniel; Guo-Zhong Tao; Sofie M. Kvarnström; Parvez Alam; M. Bishr Omary; Arno Hänninen; Diana M. Toivola Summary Keratin intermediate filament (IF) proteins are epithelial cell cytoskeletal components that provide structural stability and protection...

...Pauline B. McIntosh; Peter Laskey; Kate Sullivan; Clare Davy; Qian Wang; Deborah J. Jackson; Heather M. Griffin; John Doorbar The keratin IF network of epidermal keratinocytes provides a protective barrier against mechanical insult, it is also a major player in absorbing stress in these cells...

... family members. Previous studies, including the phenotypic analyses of knockout mice, failed to reveal the biological function of epiplakin. Using in vitro binding assays, we show here that all but one of the 16 PRDs of mouse epiplakin bind to keratins of basal keratinocytes. Nevertheless, in primary...

...Miwako Nishizawa; Ichiro Izawa; Akihito Inoko; Yuko Hayashi; Koh-ichi Nagata; Tomoya Yokoyama; Jiro Usukura; Masaki Inagaki Keratins 8 and 18 (K8/18) are major components of the intermediate filaments (IFs) of simple epithelia. We report here the identification of a novel protein termed trichoplein...

... envelope proteins involucrin, loricrin, filaggrin, and of the keratins K1 and K5 were significantly altered in cathepsin D-deficient mice. Stratum corneum morphology in cathepsin D-deficient mice was impaired, with increased numbers of corneocyte layers and faint staining of the cornified envelope only...

..., T. M. and Weber, K. ( 2001 ). Genes for intermediate filament proteins and the draft sequence of the human genome: novel keratin genes and a surprisingly high number of pseudogenes related to keratin genes 8 and 18. J. Cell Sci. 114 , 2569 -2575. Hirokawa, N., Terada, S., Funakoshi, T...

...Guo-Zhong Tao; Diana M. Toivola; Bihui Zhong; Sara A. Michie; Evelyn Z. Resurreccion; Yoshitaka Tamai; Makoto M. Taketo; M. Bishr Omary Keratin transgenic mouse models and the association of human keratin mutations with liver disease highlight the importance of keratins in protecting the liver from...

.... There are several unexpected differences. F. rubripes has a sizeable excess of keratin type I genes over keratin type II genes. Four of the six keratin type II genes map close to four keratin type I genes. Thus, a single keratin II gene cluster(as in mammals) seems excluded. Although a continuous genome sequence...

... as basic mechanisms capable of rapidly adapting the cytokeratin filament cytoskeleton to specific requirements. * Author for correspondence (e-mail: [email protected] ) 7 8 2002 © The Company of Biologists Limited 2002 2002 Tyrosine phosphorylation Keratin Intermediate...

...Li Feng; Xiangjun Zhou; Jian Liao; M. Bishr Omary ABSTRACT Glandular epithelia express the keratin intermediate filament (IF) polypeptides 8, 18 and 19 (K8/18/19). These proteins undergo significant serine phosphorylation upon stimulation with growth factors and during mitosis, with subsequent...

..., they converted reversibly to a spindle morphology and ceased K5 and K14 expression. Under these conditions, EGF deprivation induced flattening, growth arrest, and expression of α-smooth muscle actin (α-sma). Coexpression of keratins and α-sma is a hallmark of myoepithelial cells, a constituent of secretory...

..., rather than cells originated by dedifferentiation. We have previously shown that simple epithelial keratins 8 and 18 are expressed in the mesenchymal progenitor cells of the regenerating amphibian limb and in cultured cells with myogenic potential, and that their expression appears to be causally related...