... cytoplasmic splicing allows muscle cells to switch from a signaling to an anchoring and signaling-deficient phenotype through interaction with intracellular isoforms of adapter protein talin. Integrins Isoform Splicing Muscle Paxillin Adhesion Focal adhesions Fibrillar adhesions Fibronectin...

... this distinction have been debated in the literature for decades. This Review summarizes data on the functional regulation of different actin isoforms, and the mechanisms that lead to their different biological roles in vivo . We focus here on recent studies demonstrating that at least some actin functions...

...Yong Tang; Zongliang Jiang; Yan Luo; Xueming Zhao; Ling Wang; Carol Norris; Xiuchun Cindy Tian ABSTRACT Akt plays an important role in cell growth, proliferation and survival. The specific roles of the three Akt isoforms in somatic cell reprogramming have not been investigated. Here we report...

..., a Gfi-1B isoform, as essential for erythroid differentiation. Gfi-1B p32 is generated by alternative splicing and lacks the two first zinc finger domains of the protein. Selective knock down of Gfi-1B p32 compromises erythroid differentiation, whereas its ectopic expression induces erythropoiesis...

... and immediate C-terminal flanking region (TM4 - ). Examination of the genomic occludin sequence identified that the 162 bp sequence deleted in TM4 - coincided precisely with occludin exon 4, strongly suggesting that TM4 - is an alternative splice isoform generated by skipping of exon 4. Indeed, the reading...

...Peter W. Gunning; Vicki Ferguson; Karen J. Brennan; Hardeman Edna C. ABSTRACT Muscle differentiation is characterized by the induction of genes encoding contractile structural proteins and the repression of nonmuscle isoforms from these gene families. We have examined the importance...

...Toyoshi Fujimoto; Hiroshi Kogo; Ryuji Nomura; Tomoko Une ABSTRACT The relationship between caveolin-1 isoforms (α and β) and caveolar ultrastructure was studied. By immunofluorescence microscopy of human fibroblasts, caveolae were observed as dots positive for caveolin-1, but many dots labeled...

... attached, sarcomere organization was deficient, leaving the transformants flightless. Rather than affecting primarily actin-myosin interactions, our findings suggest that the β-cytoplasmic actin isoform is not competent to interact with other actin-binding proteins in the IFM that are involved...

... endothelins. Three ECE-1 isoforms, differing by their N-terminal cytoplasmic tails, are generated from a single gene. When expressed in CHO cells, they display comparable enzymatic activity but whereas ECE-1a is strongly expressed at the cell surface, ECE-1b is exclusively intracellular and ECE-1c presents...

.... , Bulinski , J. C. and Lessard , J. L. ( 1989 ). Skeletal muscle myofibrillogenesis as revealed with a monoclonal antibody to titin in combination with detection of the α- and γ-isoforms of actin . Dev. Biol . 132 , 35 – 44 . 10.1016/0012-1606(89)90202-9 Harlow , E. and Lane...

... 1993 © 1993 by Company of Biologists 1993 integrin glomerulus kidney focal contact extracellular matrix laminin isoform The regulated interaction of cells with the extracellular matrix (ECM) is critical during embryonal developmental and aberrant interactions that can...

... actin to the membrane. In this report, we describe a monoclonal antibody (Mab 6E) that was used to immunoprecipitate an isoform of filamin from extracts of chicken fibroblasts. Immunofluorescence staining of chicken fibroblasts revealed an unusual pattern: while other filamin antibodies stained...