... degradation and appearance of the matrix metalloproteinase (MMP)-generated collagen-I cleavage product reC1M, and these increases were greater than those observed upon overexpression of each protein alone, suggesting a concerted role in ECM degradation. MCT4 and CD147 colocalized with invadopodia markers...

...Sameena Parveen; Amrita Khamari; Jyothikamala Raju; Marc G. Coppolino; Sunando Datta ABSTRACT Invasion in various cancer cells requires coordinated delivery of signaling proteins, adhesion proteins, actin-remodeling proteins and proteases to matrix-degrading structures called invadopodia. Vesicular...

... adhesions (FAs), focal complexes, fibrillar adhesions, podosomes and invadopodia, contain many shared scaffolding and signaling ‘adhesome’ components, as well as distinct molecules that perform specific functions, unique to each adhesion form. In this Hypothesis, we address the pivotal roles of mechanical...

...Olivia R. Grafinger; Genya Gorshtein; Tyler Stirling; Megan I. Brasher; Marc G. Coppolino ABSTRACT Malignant cancer cells can invade extracellular matrix (ECM) through the formation of F-actin-rich subcellular structures termed invadopodia. ECM degradation at invadopodia is mediated by matrix...

... is easily adaptable for the analysis of invadopodia and associated extracellular matrix degradation, and likely also of other micron-size punctate structures. This article describes the workflow of the Poji macro, presents several examples of its applications, and also points out limitations, as well...

...Battuya Bayarmagnai; Louisiane Perrin; Kamyar Esmaeili Pourfarhangi; Xavier Graña; Erkan Tüzel; Bojana Gligorijevic ABSTRACT The process of tumor cell invasion and metastasis includes assembly of invadopodia, protrusions capable of degrading the extracellular matrix (ECM). The effect of cell cycle...

...Bettina Noll; David Benz; Yannick Frey; Florian Meyer; Manuel Lauinger; Stephan A. Eisler; Simone Schmid; Peter L. Hordijk; Monilola A. Olayioye ABSTRACT Cancer cells degrade the extracellular matrix through actin-rich protrusions termed invadopodia. The formation of functional invadopodia requires...

..., and act as scaffolds, bringing membrane and cellular components in close proximity in structures known as invadopodia or podosomes. Tks proteins, analogous to the related proteins p47 phox , p40 phox and NoxO1, also facilitate local generation of reactive oxygen species (ROS), which aid in signaling...

...Mark A. Eckert; Miguel Santiago-Medina; Thinzar M. Lwin; Jihoon Kim; Sara A. Courtneidge; Jing Yang ABSTRACT The Twist1 transcription factor promotes tumor invasion and metastasis by inducing epithelial–mesenchymal transition (EMT) and invadopodia-mediated extracellular matrix (ECM) degradation...

... an in vitro mechano-invasion assay. This enhancement of invasion is dependent on cofilin (CFL1), a known regulator of invadopodia maturation. Invadopodia are actin-rich structures present in invasive cancer cells that are enzymatically active and degrade the surrounding extracellular matrix to facilitate...

... barrier, by developing specialized actin-rich membrane protrusion structures called invadopodia. The formation of invadopodia is regulated by Rho GTPases, a family of proteins that regulates the actin cytoskeleton. Here, we describe a novel role for RhoG in the regulation of invadopodia disassembly...

...Abitha Jacob; Erik Linklater; Brian A. Bayless; Traci Lyons; Rytis Prekeris ABSTRACT Invadopodia formation and extracellular matrix degradation are key events during cancer cell invasion, yet little is known about mechanisms mediating these processes. Here, we report that Rab40b plays a key role...

... tumors, and progressively reduced in more aggressive stages of non-small-cell lung cancers. We show that SNX9 is localized at invadopodia where it directly binds the invadopodia marker TKS5 and negatively regulates invadopodia formation and function. SNX9 depletion increases invadopodia number...

...Julie Di Martino; Elodie Henriet; Zakaria Ezzoukhry; Jacky G. Goetz; Violaine Moreau; Frederic Saltel ABSTRACT Invadosomes are actin-based structures involved in extracellular matrix degradation. Invadosomes is a term that includes podosomes and invadopodia, which decorate normal and tumour cells...

.... Circulating tumor cell CTC Chemotaxis Invadopodia Human invasion signature HIS Intravasation Mena Tumor microenvironment of metastasis TMEM National Cancer Institute 10.13039/100000054 1R01 CA170507-01 National Institutes of Health 10.13039/100000002 1R01 CA170507-01...

...Pu Zhao; Yilin Xu; Yong Wei; Qiong Qiu; Teng-Leong Chew; Yibin Kang; Chonghui Cheng ABSTRACT The ability for tumor cells to spread and metastasize to distant organs requires proteolytic degradation of extracellular matrix (ECM). This activity is mediated by invadopodia, actin-rich membrane...

...Karla C. Williams; Marc G. Coppolino ABSTRACT Acquisition of an invasive phenotype is prerequisite for tumor metastasis. Degradation of the extracellular matrix (ECM), and subsequent invasion by tumor cells, is mediated, in part, through subcellular structures called invadopodia. Src-dependent...

... an essential step in metastatic progression and this is performed by cancer cell structures called invadopodia. Na V 1.5 (also known as SCN5A) Na + channels are overexpressed in breast cancer tumours and are associated with metastatic occurrence. It has been previously shown that Na V 1.5 activity enhances...

...Abitha Jacob; Jian Jing; James Lee; Pepper Schedin; Simon M. Gilbert; Andrew A. Peden; Jagath R. Junutula; Rytis Prekeris Summary Invadopodia-dependent degradation of the basement membrane plays a major role during metastasis of breast cancer cells. Basement membrane degradation is mediated...

...Daisuke Hoshino; Kevin M. Branch; Alissa M. Weaver Summary Remodeling of extracellular matrix (ECM) is a fundamental cell property that allows cells to alter their microenvironment and move through tissues. Invadopodia and podosomes are subcellular actin-rich structures that are specialized...