... in the remaining cells (Bi). (C) Although photolysed caged Ins(1,4,5)P3 (▴) increased [Ca2+]c (i), ATP (1 mM by pressure ejection; ii), however, failed to evoke [Ca2+]c rises as indicated by F/F0 (i) in cells voltage clamped at −30 mV (iii). We hypothesised that ATP may inhibit Ins(1,4,5) P 3 R-mediated...

... at lines of width 1 pixel. The position of each measurement (regions 1-9) line is shown (C, left panel) at a width of two pixels to facilitate visualization. The second photolysis site lies between regions 8-9 – that is, ∼75 μm away from photolysis site 1. Fig. 2. Localized depletion of the Ins(1,4,5)P3...

... through Ins(1,4,5)P3Rs or RyRs in the inner nuclear membrane (INM) or through the NPCs when released through Ins(1,4,5)P3Rs or RyRs in the outer nuclear membrane (ONM). Three messengers can be produced locally inside the NE: Ins(1,4,5)P3 generated by phospholipase C (PLC) or cADPR and NAADP generated...

.... Fig. 5. Temporal patterns of Ca2+ changes induced by 5-HT and Ins(1,4,5)P3. The oocyte injected with 10 kDa (A, into the cytoplasm) or 70 kDa CGD (B, into the nucleus) was exposed to 5-HT (final concentration: 100 nM) in FSW. In C, the oocyte injected with 10 kDa CGD plus 600 μM caged Ins(1,4,5)P3...

...) and mitochondria (in green). Red arrows indicate the direction of the waves, whereas the postulated sites of Ins(1,4,5)P3production are symbolized as purple dots. sa: sperm aster; cp: contraction pole. (E) Postulated temporal variations of[Ins(1,4,5)P3]c, which may underlie the activity of the meiotic Ca2+ wave...