... compared with that in untransfected cells under the same tonicity (one-way ANOVA with Bonferroni's multiple comparison test). Fig. 4. Identification of importin and exportin for NFAT5 nuclear import and export. (A) In vitro nuclear import assay of His–SV40 NLS –AcGFP and His–NFAT5 171–250...

...Li Guo; Khamsah Suryati Mohd; He Ren; Guangwei Xin; Qing Jiang; Paul R. Clarke; Chuanmao Zhang ABSTRACT Importin-α serves as an adaptor linking importin-β to proteins carrying a nuclear localization sequence (NLS). During interphase, this interaction enables nuclear protein import, while in mitosis...

... of the nuclear pore. Here, we focused on the characteristic enrichment of prolines in the middle of the outer α-helices of importin-β. When these prolines were substituted with alanine, nuclear transport activity was reduced drastically in vivo and in vitro , and caused a severe defect in mitotic progression...

...-hydroxylase HIF NLS NES Importin CRM1 Due to an imbalance in oxygen supply and demand, solid tumours commonly evolve hypoxic regions associating with malignant cancer phenotype and poor patient survival. As a result, hypoxia stimulated pathways, including the hypoxia-inducible factor (HIF...

... localizes to the ciliary base and this requires the dual acylation of the N-terminus, but the precise mechanism by which RP2 is trafficked to the cilia is unknown. Here we have characterized an interaction between RP2 and Importin β2 (transportin-1), a member of the Importin-β family that regulates nuclear...

... are imported into or exported out of the nucleus. Here we show that several importins mediate the nuclear import of the human Snail proteins and we identify a unique nuclear localization signal (NLS), recognized by all the importins, that has been conserved during the evolution of the Snail family. This NLS...

... GTPase-activating protein RanGAP. The result is an intracellular concentration gradient of RanGTP that equips eukaryotic cells with a `genome-positioning system' (GPS). The binding of RanGTP to nuclear transport receptors (NTRs) of the importin β superfamily mediates the effects of the gradient...

... sequence-tagged CapG-EGFP fails to induce invasion, whereas point mutations in the nuclear export sequence permitting nuclear re-entry restore cellular invasion. Nuclear import of CapG is energy-dependent and requires the cytosolic receptor importin β but not importin α. Nuclear CapG does not possess...

..., was efficiently imported into the nucleus in vitro by two members of the importin β superfamily of transport receptors, importin 8 and transportin; SRP19 was also imported less efficiently by several other members of the importin β family. Although transportin is known to import a variety of proteins, SRP19...