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Keywords: Immunofluorescence
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Journal Articles
J Cell Sci (2020) 133 (12): jcs246330.
Published: 24 June 2020
... in situ hybridization (FISH) with single-molecule RNA FISH (smFISH) and immunofluorescence to study nuclear architecture-dependent gene regulation on a cell-by-cell basis. We further combine our method with imaging-based cell cycle staging to correlate nuclear architecture with gene expression across...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (7): jcs239756.
Published: 8 April 2020
... of mRNA ex vivo in complex brain cells, such as astrocytes and microglia, in physiological and pathological conditions. Astrocytes Microglia Hippocampus mRNA In situ hybridization Immunofluorescence ImageJ APPswe/PS1dE9 mouse Alzheimer's disease GFAP Fondation pour la Recherche...
Includes: Supplementary data
Journal Articles
Journal Articles
J Cell Sci (2005) 118 (1): 175–186.
Published: 1 January 2005
... certain circumstances, can be found in the cytoplasm. We show here that PPARα interacts with the centrosome-associated protein CAP350. CAP350 also interacts with PPARδ, PPARγ and liver-X-receptor α, but not with the 9-cis retinoic acid receptor, RXRα. Immunofluorescence analysis indicated that PPARα...
Includes: Multimedia, Supplementary data
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Journal Articles
Journal Articles
J Cell Sci (2001) 114 (8): 1491–1503.
Published: 15 April 2001
... as the cellular cofactor for the human immunodeficiency virus transactivator Tat protein. In this study, we examined the subcellular distribution of Cdk9 and cyclin T1 using high resolution immunofluorescence microscopy and found that Cdk9 and cyclin T1 localized throughout the non-nucleolar nucleoplasm...
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Journal Articles
Journal Articles
J Cell Sci (1999) 112 (7): 1055–1064.
Published: 1 April 1999
...Suisheng Zhang; Christine Herrmann; Frank Grosse ABSTRACT Nuclear DNA helicase II (NDH II), alternatively named RNA helicase A, seems to function as a pre-mRNA and mRNA binding protein in human cells. Immunofluorescence studies of NDH II gave a highly diffused nucleoplasmic staining...
Journal Articles
J Cell Sci (1997) 110 (22): 2833–2844.
Published: 15 November 1997
...-substratum Adhesion Keratocyte Immunofluorescence IRM (interference reflection microscopy) The formation of adhesive contacts between the ventral cell surface and the substratum is essential for locomotion of cells along a surface. Cells generally make two kinds of adhesive contact...
Journal Articles
Journal Articles
J Cell Sci (1996) 109 (8): 2079–2088.
Published: 1 August 1996
... as immunofluorescence and confocal laser microscopy with highly specific antibodies against various chicken CK isoenzymes revealed that cytosolic brain-type CK isoenzyme (B-CK) is the only CK isoenzyme in rooster seminal plasma, while three isoenzymes, cytosolic B-CK, sarcomeric mitochondrial CK (Mib-CK), and a variant...
Journal Articles
J Cell Sci (1995) 108 (11): 3397–3403.
Published: 1 November 1995
... ends of the thin filaments. * Author for correspondence at address 1 31 05 1995 07 08 1995 © 1995 by Company of Biologists 1995 cardiac rhodamine fluorescence microscopy immunofluorescence It has been reported that the binding of fluorescent phallotox-ins...
Journal Articles
J Cell Sci (1995) 108 (4): 1391–1396.
Published: 1 April 1995
... by Company of Biologists 1995 lampbrush chromosome K-protein homonucleotide binding immunofluorescence chromosome mapping It is now well known that both snRNP and hnRNP complexes play important roles in the complicated and highly regulated processing of nuclear pre-mRNAs. Some steps...
Journal Articles
J Cell Sci (1995) 108 (1): 85–95.
Published: 1 January 1995
... induced and in striated muscle only the 51 kDa isoform was observed. Immunofluorescence showed that annexin VII was located in the cytosol of mononucleated and fused polynucleated cultured cells, whereas in striated muscle, annexin VII was located preferentially at the plasma membrane and the transverse...
Journal Articles
J Cell Sci (1994) 107 (11): 3097–3103.
Published: 1 November 1994
... 27 07 1994 © 1994 by Company of Biologists 1994 DNA replication immunofluorescence bromodeoxyuridine labelling cell cycle chromatin structure DNA replication in eukaryotic cell nuclei is achieved through the simultaneous activation of a number of groups of adjacent...
Journal Articles
J Cell Sci (1994) 107 (9): 2417–2426.
Published: 1 September 1994
... kinesin antibodies in conjunction with subcellular fractionation techniques, immunoblot analysis and immunofluorescence microscopy to conduct a detailed study on the association of kinesin with the Golgi and other membranous organelles in a polarized epithelial cell, the primary rat hepatocyte. We found...
Journal Articles
J Cell Sci (1994) 107 (3): 507–515.
Published: 1 March 1994
... proteins. Chimeric and endogenous Gα i proteins were detected in cells using specific carboxy-terminal peptide antibodies. Immunofluorescence staining was used to localize en-dogenous and chimeric Gα i proteins in LLC-PK 1 cells. The staining of chimeric proteins was detected as an increased intensity...
Journal Articles