... cellular landscape. Currently, this is hindered by our limited ability to combine multiple cellular markers. To overcome this, we adapted a highly multiplexed immunofluorescence (IF) technique called ‘Iterative Bleaching Extends Multiplexity’ (IBEX) to the zebrafish retina. We optimised fluorescent...

... in situ hybridization (FISH) with single-molecule RNA FISH (smFISH) and immunofluorescence to study nuclear architecture-dependent gene regulation on a cell-by-cell basis. We further combine our method with imaging-based cell cycle staging to correlate nuclear architecture with gene expression across...

... of mRNA ex vivo in complex brain cells, such as astrocytes and microglia, in physiological and pathological conditions. Astrocytes Microglia Hippocampus mRNA In situ hybridization Immunofluorescence ImageJ APPswe/PS1dE9 mouse Alzheimer's disease GFAP Fondation pour la Recherche...

... Genome stability Rothmund-Thomson syndrome (RTS) RecQ helicases Immunofluorescence Promyelotic leukaemia protein (PML) Rad51 Genome instability is thought to play a major role in the development and progression of cancer, and has also been implicated in the aging process. One important...

... certain circumstances, can be found in the cytoplasm. We show here that PPARα interacts with the centrosome-associated protein CAP350. CAP350 also interacts with PPARδ, PPARγ and liver-X-receptor α, but not with the 9-cis retinoic acid receptor, RXRα. Immunofluorescence analysis indicated that PPARα...

...Roland Brock; Thomas M. Jovin Epidermal growth factor receptor (EGFR, erbB1) activation and translocation of the Shc adaptor protein to activated receptors were analyzed at the subcellular level by dual-label immunofluorescence and confocal laser scanning microscopy in conjunction with a new...

... of these three amino acids to alanine. * Author for correspondence (e-mail: [email protected] ) 13 02 2001 © 2001 by Company of Biologists 2001 TGF-β receptors Endocytosis Di-leucine Immunofluorescence Clathrin-coated-pit-mediated endocytosis is a major mechanism...

... as the cellular cofactor for the human immunodeficiency virus transactivator Tat protein. In this study, we examined the subcellular distribution of Cdk9 and cyclin T1 using high resolution immunofluorescence microscopy and found that Cdk9 and cyclin T1 localized throughout the non-nucleolar nucleoplasm...

... recognize both mouse and human BLM in western blots of cell lines and in successive developmental stages of spermatocytes, but fail to detect BLM protein in a cell line with a C-terminally truncated protein. BLM protein expression and location are detected by immunofluorescence and immunoelectron microscopy...

... Urokinase RAP Endocytosis Immunofluorescence The low density lipoprotein (LDL) receptor family consists of structurally related endocytic receptors that include the LDL receptor, apoE receptor 2, LDL receptor-related protein (LRP also know as LRP-1), megalin (also known as gp330 or LRP-2...

...Suisheng Zhang; Christine Herrmann; Frank Grosse ABSTRACT Nuclear DNA helicase II (NDH II), alternatively named RNA helicase A, seems to function as a pre-mRNA and mRNA binding protein in human cells. Immunofluorescence studies of NDH II gave a highly diffused nucleoplasmic staining...

... of extension and retraction are not as highly coordinated. The composition of close substratum contacts was investigated by immunofluorescence staining of fixed keratocytes using antibodies against known components of focal adhesions, such as β1-integrin, talin, vinculin, α-actinin, paxillin and the focal...

.... It appears as a 90 and 91 kDa doublet in western blots and is translated from a single 2.7 kb mRNA. Antibodies raised against cellular and bacterially-expressed protein give identical cytoplasmic immunofluorescence and immunoblot results. Mitofilin co-localizes with mitochondria in immunofluorescence...

... as immunofluorescence and confocal laser microscopy with highly specific antibodies against various chicken CK isoenzymes revealed that cytosolic brain-type CK isoenzyme (B-CK) is the only CK isoenzyme in rooster seminal plasma, while three isoenzymes, cytosolic B-CK, sarcomeric mitochondrial CK (Mib-CK), and a variant...

... ends of the thin filaments. * Author for correspondence at address 1 31 05 1995 07 08 1995 © 1995 by Company of Biologists 1995 cardiac rhodamine fluorescence microscopy immunofluorescence Previous fluorescence imaging studies have shown preferen-tial binding...

... by Company of Biologists 1995 lampbrush chromosome K-protein homonucleotide binding immunofluorescence chromosome mapping It is now well known that both snRNP and hnRNP complexes play important roles in the complicated and highly regulated processing of nuclear pre-mRNAs. Some steps...

... induced and in striated muscle only the 51 kDa isoform was observed. Immunofluorescence showed that annexin VII was located in the cytosol of mononucleated and fused polynucleated cultured cells, whereas in striated muscle, annexin VII was located preferentially at the plasma membrane and the transverse...

... of the replication clusters through all stages of the three subsequent cell cycles may be an indication in favour of the hypothesis that such clusters represent structurally stable replicon complexes held together by the nuclear matrix and the chromosome scaffold. DNA replication immunofluorescence...

... kinesin antibodies in conjunction with subcellular fractionation techniques, immunoblot analysis and immunofluorescence microscopy to conduct a detailed study on the association of kinesin with the Golgi and other membranous organelles in a polarized epithelial cell, the primary rat hepatocyte. We found...

... proteins. Chimeric and endogenous Gα i proteins were detected in cells using specific carboxy-terminal peptide antibodies. Immunofluorescence staining was used to localize en-dogenous and chimeric Gα i proteins in LLC-PK 1 cells. The staining of chimeric proteins was detected as an increased intensity...