... formation is not correlated with the sizes of pre-existing RNA processing bodies (P-bodies) but that the two structures often associated rapidly. Immunoelectron microscopy revealed a previously unidentified characteristic of the fine structures of Arabidopsis stress granules and P-bodies: the lack...

... in mouse spermatocytes using immunoelectron microscopy. Distribution of immuno-gold particles in a lateral view of the synaptonemal complex, supported by protein interaction data, suggest that the N-terminal region of SYCP1 and SYCE3 form a joint bilayered central structure, and that SYCE1 and SYCE2...

... 11 2003 © The Company of Biologists Limited 2004 2004 VPS4 Clathrin Endosomes Membrane domains Immunoelectron microscopy Based on the time-dependent accessibility for endocytic tracers, the endocytic pathway can be subdivided into early endosomes (EEs), late endosomes (LEs...

... proteins Gene silencing Chromatin Immunoelectron microscopy Transcription In differentiated eukaryotic cells the transcriptional activity of most genes is permanently suppressed. This silenced state is faithfully transmitted to the daughter cells during cell division and constitutes the basis...

... along the length of the cell is proposed to be a post-Golgi and probably late endosomal/lysosomal compartment. Using biotinylation experiments, FACS analysis and quantitative immunoelectron microscopy it was found that, at comparable expression levels, 73-75% of the two GPI-anchored proteins but only 13...

... recognize both mouse and human BLM in western blots of cell lines and in successive developmental stages of spermatocytes, but fail to detect BLM protein in a cell line with a C-terminally truncated protein. BLM protein expression and location are detected by immunofluorescence and immunoelectron microscopy...

...-mail: [email protected] ) 10 03 1999 26 01 1999 © 1999 by Company of Biologists 1999 DEXH family Immunoelectron microscopy Immunofluorescence Mitosis MLE hnRNP A 1 poly(A) RNA Sc-35 Nuclear DNA helicase II (NDH II) was originally isolated from calf thymus...

... proliferation Pollen grain Immunoelectron microscopy In situ hybridization MAPKs constitute a family of serine/threonine protein kinase that has been highly conserved during evolution, with members found in such diverse organisms as mammals, Xenopus and yeast ( Pelech and Shanghera, 1992 ; Blumer...

... contributed equally to this study ‡ Author for correspondence (e-mail: [email protected] ) 28 10 1998 14 09 1998 © 1998 by Company of Biologists 1998 ER-Golgi intermediate compartment ERGIC-53 β-COP Immunoelectron microscopy Protein recycling Protein transport...

.... To elucidate this region’s contribution to titin elasticity, we measured the elastic properties of the N-terminal I-band Ig region by using immunofluorescence/immunoelectron microscopy and myofibril mechanics and tried to simulate the results with a model of entropic polymer elasticity. Rat psoas myofibrils...

... architecture of this unique microtubule-organizing center, we have isolated and sequenced the gene encoding γ-tubulin and have studied its localization in the Dictyostelium centrosome using immunofluorescence and postembedding immunoelectron microscopy. D. discoideum possesses a single copy of a γ-tubulin gene...

..., Biotechnology Building, Ithaca, NY 14853, USA † Author for correspondence 31 10 1994 09 03 1995 © 1995 by Company of Biologists 1995 β 2 -microglobulin class I antigen Fc receptor immunoelectron microscopy immunoglobulin G major histocompatibility complex neonatal rat...

... a polyclonal antibody against the purified human p63 protein to reassess the subcellular distribution of p63 by confocal immunofluorescence, immunoelectron microscopy, and cell fractionation. Double immunofluorescence of COS cells showed significant colocalization of p63 and a KDEL-containing lumenal ER marker...

... for correspondence 07 11 1994 11 01 1995 © 1995 by Company of Biologists 1995 rab protein ER-to-Golgi transport pre-Golgi compartment secretory pathway immunoelectron microscopy Work carried out in the past few years has resulted in the identification of a large number...

... 10 1994 © 1995 by Company of Biologists 1995 cell nucleus immunoelectron microscopy lamina nuclear matrix The nuclear lamina is a protein mesh underlying the nuclear membrane that remains associated with the residual nuclear envelope after extraction with non-ionic...

... amastigote GIPL, EPiM3 (∼2×10 7 molecules/cell), is located at the parasite cell surface, in the flagellar pocket and in lysosomal membranes, but not on host cell structures as shown by immunofluorescence and immunoelectron microscopy. In addition, amastigotes in infected Balb/c mice contain a glycolipid...

... of Biologists 1994 secretion human serum albumin hepatoma cell immunoelectron microscopy The complex pathway taken by soluble secretory proteins inside cells, starting with their synthesis and ending with their release at the cell surface, was laid out some time ago by Palade (1975...

... of the caudal head in these steps. There was little dynein fluorescence in mature spermatozoa. Immunoelectron microscopy showed positive reactions in the nuclear envelope and the inner region of the microtubular manchette. These observations suggest that cytoplasmic dynein, possibly bound to the nuclear...

... and by immunoelectron microscopy the subcellular distribution of both MHC class I and class II molecules in mouse (Balb/c and C57BL/6 strains) bone marrow-derived macrophages infected for 12 to 48 hours with Leishmania amazonensis amastigotes and activated with gamma interferon to determine the intra-cellular sites...

... proteins. * Author for correspondence 09 08 1993 13 08 1993 © 1993 by Company of Biologists 1993 myofibrillogenesis intermediate filament proteins titin myosin nebulin α-actinin immunolocalization immunoelectron microscopy In the sarcomeric structures...