... and secretory domain at the interface with their target, the immune synapse (IS). During IS formation, CTLs acquire a transient polarity, marked by re-orientation of the centrosome and microtubule cytoskeleton toward the IS, thus directing the transport and delivery of the lytic granules to the target cell...

...Keith P. Eidell; Alenka Lovy; Nicholas R. Sylvain; Frank A. Scangarello; Hayley I. Muendlein; Michael J. Ophir; Ken Nguyen; Maria-Cristina Seminario; Stephen C. Bunnell ABSTRACT Integrin engagement within the immune synapse enhances T cell activation, but our understanding of this process...

...-opted by the non-ciliated T cell to orchestrate polarized endosome recycling and to sustain signaling during immune synapse formation. Here, we investigated the potential role of Bardet–Biedl syndrome 1 protein (BBS1), an essential core component of the BBS complex that cooperates with the IFT system...

...Daniel Friedman; Poppy Simmonds; Alexander Hale; Leoma Bere; Nigel W. Hodson; Michael R. H. White; Daniel M. Davis ABSTRACT Natural killer (NK) cells can kill infected or transformed cells via a lytic immune synapse. Diseased cells may exhibit altered mechanical properties but how this impacts NK...

... characteristics, needs careful reconsideration. Actin cytoskeleton Jurkat cells Immune synapse Primary T-cells Wellcome Trust 10.13039/100010269 212343/Z/18/Z Engineering and Physical Sciences Research Council 10.13039/501100000266 EP/S004459/1 Engineering...

.../by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Summary: Immune cell–cell interactions are reconstituted in free-standing vesicles, allowing the spatiotemporal aspects of immune synapse formation...

...Jia C. Wang; Jeff Y.-J. Lee; Sonja Christian; May Dang-Lawson; Caitlin Pritchard; Spencer A. Freeman; Michael R. Gold ABSTRACT B cells that bind antigens displayed on antigen-presenting cells (APCs) form an immune synapse, a polarized cellular structure that optimizes the dual functions of the B...

...Nele M. G. Dieckmann; Gordon L. Frazer; Yukako Asano; Jane C. Stinchcombe; Gillian M. Griffiths ABSTRACT The immune synapse provides an important structure for communication with immune cells. Studies on immune synapses formed by cytotoxic T lymphocytes (CTLs) highlight the dynamic changes...

...Francesca Finetti; Laura Patrussi; Donatella Galgano; Chiara Cassioli; Giuseppe Perinetti; Gregory J. Pazour; Cosima T. Baldari ABSTRACT IFT20, a component of the intraflagellar transport (IFT) system that controls ciliogenesis, regulates immune synapse assembly in the non-ciliated T-cell...

...Francesca Finetti; Laura Patrussi; Giulia Masi; Anna Onnis; Donatella Galgano; Orso Maria Lucherini; Gregory J. Pazour; Cosima T. Baldari ABSTRACT T cell activation requires sustained signaling at the immune synapse, a specialized interface with the antigen-presenting cell (APC) that assembles...

... cell receptor (TCR) organizes a structured signaling platform at the T cell/APC contact area, known as the immune synapse (IS). Recruitment of PLCγ (phospholipase C γ) to the IS allows rapid hydrolysis of PIP2 (phosphatidylinositol 4,5-bisphosphate), which results in intracellular Ca 2+ release...

... in triplicate. *P<0.05; **P<0.01. Fig. 7. Syntenin-1–M-RIP regulates F-actin polymerization at the immune synapse. ( A ) Antigen-induced conjugates were stained for syntenin-1 and CD3 or for M-RIP and F-actin. Maximal projections of the confocal image stacks are shown, together...

... immune response depends on the interaction of T cells and antigen-presenting cells at the immune synapse. Formation of the immune synapse and the subsequent T-cell activation are highly dependent on the actin cytoskeleton. In this work, we describe that T cells express drebrin, a neuronal actin-binding...

... of the relevant actin-regulatory proteins, and significant progress has been made in our understanding of how these proteins choreograph molecular movements associated with T-cell activation. Proteins such as WASp, WAVE2, HS1 and cofilin direct the formation of a cortical actin scaffold at the immune synapse...

... is recruited to immune synapses after a few seconds of cell conjugate formation, the intracellular pool is first polarized and then recruited after a few minutes. We further show that LAT intracytoplasmic amino acid residues, particularly the Tyr136, 175, 195 and 235 residues, are required for its own...