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Keywords: Image correlation spectroscopyClose
Claire M. Brown, Benedict Hebert, David L. Kolin, Jessica Zareno, Leanna Whitmore, Alan Rick Horwitz, Paul W. Wiseman
J Cell Sci (2006) 119 (24): 5204–5214.
Published: 15 December 2006
... and their complex network of interactions make it difficult to assess their role in cell migration. We apply a novel image analysis tool, spatio-temporal image correlation spectroscopy (STICS), to quantify the directed movements of adhesion-related proteins and actin in protrusions of migrating cells. The STICS...
Includes: Multimedia, Supplementary data
J Cell Sci (2005) 118 (24): 5825–5834.
Published: 15 December 2005
.... Here, the nuclear distribution of microinjected fluorescein-labeled dextrans of 42 kDa to 2.5 MDa molecular mass was used to characterize the chromatin accessibility in dependence on histone acetylation. Measurements of the fluorescein-dextran sizes were combined with an image correlation spectroscopy...
J Cell Sci (2005) 118 (3): 643–650.
Published: 1 February 2005
... by a donkey anti-goat RRX secondary antibody. The collected images show 100% colocalization with the receptors in addition to some background fluorescence. To measure the distribution of the BMP receptors on the cell surface, we used image correlation spectroscopy (ICS) and ICCS. A431 cells were cultured...
Katalin Fejes Tóth, Tobias A. Knoch, Malte Wachsmuth, Monika Frank-Stöhr, Michael Stöhr, Christian P. Bacher, Gabriele Müller, Karsten Rippe
J Cell Sci (2004) 117 (18): 4277–4287.
Published: 15 August 2004
... expressing histone H2A, which was fused to enhanced yellow fluorescent protein. The globally increased histone acetylation caused a reversible decondensation of dense chromatin regions and led to a more homogeneous distribution. These structural changes were quantified by image correlation spectroscopy...
J Cell Sci (2003) 116 (16): 3277–3284.
Published: 15 August 2003
... explore the importance of BMP receptor clustering in signalling, image correlation spectroscopy has been used to investigate the clustering and distribution of BMP receptors at the surface of the cell membrane. Here we demonstrate that the co-expression of the BMP-type-II receptor (BRII) influences...