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Keywords: Image analysis
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Journal Articles
J Cell Sci (2022) 135 (10): jcs259511.
Published: 17 May 2022
...-edge microscopy technologies permit the routine acquisition of 3D datasets, there is currently a limited number of open-source software packages to analyse such images. Here, we describe General Image Analysis of Nuclei-based Images (GIANI; https://djpbarry.github.io/Giani), new software...
Includes: Supplementary data
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Imaging
J Cell Sci (2022) 135 (6): jcs259685.
Published: 11 March 2022
...-Carreras , I. , Frise , E. , Kaynig , V. , Longair , M. , Pietzsch , T. , Preibisch , S. , Rueden , C. , Saalfeld , S. , Schmid , B. et al. ( 2012 ). Fiji: an open-source platform for biological-image analysis . Nat. Methods 9 , 676 - 682 . 10.1038/nmeth.2019...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2021) 134 (6): jcs254151.
Published: 30 March 2021
... Summary: This Review dissects what image processing parameters should be included in a publication, and the reasons they are essential for scientific reproducibility and data verification. Accurate reporting Data reproducibility Image analysis Image processing Microscopy Image...
Includes: Supplementary data
Journal Articles
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Polarity
J Cell Sci (2020) 133 (22): jcs252064.
Published: 30 November 2020
... regulators, and membrane and cell wall remodelers. Because tip growth speeds may vary extensively between life cycles or species, we asked whether the local amount of specific polar elements could determine or limit tip growth speeds. Using the model fission yeast, we developed a quantitative image analysis...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2020) 133 (21): jcs250027.
Published: 5 November 2020
... or financial interests. © 2020. Published by The Company of Biologists Ltd 2020 Summary: A guide on how to appropriately design hypothesis-driven, quantitative fluorescence microscopy experiments using a reverse logic. Experimental design Hypothesis Image analysis Microscope choice...
Journal Articles
In collection:
Imaging
J Cell Sci (2018) 131 (3): jcs211847.
Published: 8 February 2018
... discusses important considerations, including applications to super-resolution microscopy. Image analysis Manders Pearson Co-localization Fluorescence microscopy A common task in cell biology consists of assessing to what extent two biomolecules or structures are associated with each other...
Journal Articles
J Cell Sci (2017) 130 (12): 2066–2077.
Published: 15 June 2017
... the importance of image analysis to enhance super-resolution techniques. Structured illumination microscopy Image analysis Chromatin Nucleus Histone modification Nuclear pore complexes The nuclear periphery (NP) represents a complex compartment of substantial importance for chromatin...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (20): 3714–3719.
Published: 15 October 2015
... keratinocytes and skin. Super-resolution fluorescence microscopy Keratinocyte Hemidesmosome Keratin filaments Image analysis Classic type-I hemidesmosomes are rivet-like structures, which play a crucial role in maintaining tissue integrity and resisting mechanical force ( Walko et al., 2015...
Includes: Supplementary data
Journal Articles
J Cell Sci (2007) 120 (21): 3715–3722.
Published: 1 November 2007
... of the necessary hardware and software components, image analysis, statistical analysis and data presentation tools. Given the increasing availability of such hardware and software, these types of experiments have come within the reach of individual labs, heralding many interesting new ways of acquiring biological...