... Analysis Toolbox (GAT), an image analysis tool designed for characterization of enteric neurons and their neurochemical coding using two-dimensional images of GI wholemount preparations. GAT is developed in Fiji, has a user-friendly interface, and offers rapid and accurate segmentation via custom deep...

... Analysis Machine learning Microscopy Bioinformatics Image analysis Knut och Alice Wallenbergs Stiftelse https://doi.org/10.13039/501100004063 Over the past two decades, light microscopy has benefited from significant advancements in optical design and sample preparation...

.... , Kausler , B. X. , Haubold , C. , Schiegg , M. , Ales , J. , Beier , T. , Rudy , M. et al. ( 2019 ). ilastik: interactive machine learning for (bio)image analysis . Nat. Methods 16 , 1226 - 1232 . 10.1038/s41592-019-0582-9 Braig , S. , Schmidt , B. U. S...

... distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Summary: A flexible image analysis tool...

... drift correction Live imaging Image analysis ImageJ Fiji Academy of Finland http://dx.doi.org/10.13039/501100002341 338537 337531 332402 Syöpäjärjestöt http://dx.doi.org/10.13039/501100006383 Åbo Akademi http://dx.doi.org/10.13039/501100008019...

...-edge microscopy technologies permit the routine acquisition of 3D datasets, there is currently a limited number of open-source software packages to analyse such images. Here, we describe General Image Analysis of Nuclei-based Images (GIANI; https://djpbarry.github.io/Giani), new software...

... Fluorescence imaging Image analysis Kinase Signaling Summary: High-content biosensor imaging with kinase translocation reporters reveals substantial cell-to-cell heterogeneity in kinase activity driven by G-protein-coupled receptors. © 2022. Published by The Company of Biologists Ltd 2022...

... Summary: This Review dissects what image processing parameters should be included in a publication, and the reasons they are essential for scientific reproducibility and data verification. Accurate reporting Data reproducibility Image analysis Image processing Microscopy Image...

... regulators, and membrane and cell wall remodelers. Because tip growth speeds may vary extensively between life cycles or species, we asked whether the local amount of specific polar elements could determine or limit tip growth speeds. Using the model fission yeast, we developed a quantitative image analysis...

... or financial interests. © 2020. Published by The Company of Biologists Ltd 2020 Summary: A guide on how to appropriately design hypothesis-driven, quantitative fluorescence microscopy experiments using a reverse logic. Experimental design Hypothesis Image analysis Microscope choice...

...: Image co-localization analysis is widely used but often misunderstood. This Review discusses important considerations, including applications to super-resolution microscopy. Image analysis Manders Pearson Co-localization Fluorescence microscopy Gordon and Betty Moore Foundation...

.... This demonstrated the importance of image analysis to enhance super-resolution techniques. Structured illumination microscopy Image analysis Chromatin Nucleus Histone modification Nuclear pore complexes Grantová Agentura České Republiky 10.13039/501100001824 15-08835Y RVO: 68378050...

... in cultured keratinocytes and skin. Super-resolution fluorescence microscopy Keratinocyte Hemidesmosome Keratin filaments Image analysis References Borradori , L. and Sonnenberg , A. ( 1999 ). Structure and function of hemidesmosomes: more than simple adhesion complexes . J...

... of the necessary hardware and software components, image analysis, statistical analysis and data presentation tools. Given the increasing availability of such hardware and software, these types of experiments have come within the reach of individual labs, heralding many interesting new ways of acquiring biological...

... of endocytosis, i.e. macropinocytosis and phagocytosis. * Author for correspondence (e-mail: [email protected] ) 03 07 2000 22 08 2000 © 2000 by Company of Biologists 2000 α-Actinin Actin Macropinocytosis Ruffling Phagocytosis Image analysis Ruffling, macropinocytosis...

... with the chromatin density determined by Hoechst 33342 or propidium iodide staining, we present evidence for the existence of three HMG-I sub-populations whose contribution to the total fluorescence can be determined using a newly developed quantitative co-localization image analysis program: foci that correspond...

... in the transcriptionally active regions of Drosophila polytene chromosomes. * Author for correspondence (e-mail: [email protected] ) 22 09 1994 27 01 1995 © 1995 by Company of Biologists 1995 chromatin interphase in situ hybridization image analysis computational optical...

... was analysed using image analysis techniques. We found that RNA synthesis occurs predominantly outside domains where replication takes place, irrespective of the particular stage of S-phase. This demonstrates that in S-phase light-microscopically resolvable nuclear domains exist that are characterized...

...-212, Meibergdreef 9 1105 AZ Amsterdam, The Netherlands 29 01 1992 31 07 1992 © 1992 by Company of Biologists 1992 DNA replication chlorodeoxyuridine iododeoxyuridine confocal microscopy image analysis Early studies have shown that DNA synthesis at the molecular...

... technical assistance. REFERENCES Bereiter-Hahn , J. ( 1985 ). Computer assisted microscope interferometry by image analysis of living cells . In Advances in Microscopy. Progress in Clinical and Biological Research , vol. 196 (ed. R. R. Cowden & F. W. Harrison ), pp. 27...