...) for each of the indicated pairs of stimuli. Results (C and E) show means±s.e.m. from 10−18 cells. Fig. 7. Carbachol and photolysis of ci-IP3 stimulate the same Ca2+ release sites. (A) Cells loaded with ci-IP3 were sequentially stimulated with carbachol (10 μM) and a UV-flash (120 ms), with either...

... <0.01; *** P <0.001 (Student's t -test). Fig. 3. Ca2+ responses induced by histamine and IP3 ester are enhanced in the K-RasG13D-deleted HKH2 cell line. (Ai) Representative Ca2+ traces of HCT116 and HKH2 cells exposed to 1 µM histamine. Histamine was applied in the Ca2+-free imaging buffer. (Aii...

... in human neutrophils by inositol 1,4,5-trisphosphate (IP3) binding: differential effects of micro-injected IP3 receptor antagonists.   Biochem. J.   355 , 139 – 143 . 10.1042/0264-6021:3550139 Dewitt   S. , Hallett   M. B. (2002). Cytosolic free Ca 2+ changes and calpain activation...

... by focal Ca 2+ entry through basal membrane patch. Cell 88 , 49 -55. Moran, A. and Turner, R. J. (1996). Evidence that quantal Ca2+ release in HSG cells is not due to `all-or-none' release from discrete Ca2+ stores with differing sensitivities to IP3. Mol. Cell. Biochem. 158 , 53 -56...

... analysis of immuno-precipitated GFP-IP3BD fusion protein or GFP alone with GFP-specific Ab. (C) [3H]Ins(1,4,5)P3-([3H] IP3) binding assay using agarose beads coupled to protein A either with GFP alone or with GFP-IP3BD fusion protein. Inhibition of specific [3H]Ins(1,4,5)P3-binding was measured...

... of differentiation and/or the lack of Gα2, we inhibited the IP3-dependent pathway with compound U73122 and found that Ca 2+ entry was blocked, whereas a closely related inactive compound, U73343, did not alter the response. In agreement with this, NBD-Cl, an inhibitor of Ca 2+ uptake into the IP3-sensitive store...

... at 5 8 or less. Inositol bisphosphate (IP2) showed a transient shallow peak at about 20 s. When the events of signal transmission were slowed down by incubation at 4°C, the rapidly formed IP3 peak could be consistently seen at 5 s after stimulation and the second peak at 25–30 s. Further resolution...

...-permeabilized amoebae. The data suggest that cytoskeletal actin polymerization occurs in normal cells as a result of IP3 formation in response to cell surface receptor stimulation and the consequent release of Ca 2+ from internal stores. The finding that IP 3 does not bring about a rapid decrease...