... radiation (IR). Here, we show that inhibition of homologous recombination repair (HRR) by RIF1 occurs in a dose-dependent manner and is controlled via DNA replication. RIF1 inhibits both DNA end resection and RAD51 accumulation after exposure to high doses of IR. Contrastingly, HRR inhibition by RIF1...

... stability during DNA replication and chromosome segregation. Depletion of USP9X leads to DNA double-strand breaks, some of which are triggered by replication fork collapse. Here, we identify USP9X as a novel regulator of homologous recombination (HR) DNA repair in human cells. By performing cellular HR...

...Thomas Kusch ABSTRACT Homologous recombination is required for reciprocal exchange between homologous chromosome arms during meiosis. Only select meiotic recombination events become chromosomal crossovers; the majority of recombination outcomes are noncrossovers. Growing evidence suggests...

... and enhances DSB repair. Using DR-GFP and EJ5-GFP reporter systems, we demonstrate that BRG1 facilitates homologous recombination repair rather than nonhomologous end-joining (NHEJ) repair. Moreover, the BRG1–RAD52 complex mediates the replacement of RPA with RAD51 on single-stranded DNA (ssDNA) to initiate...

... instability. Rad52 is known to bind to replication protein A (RPA)-coated ssDNA, replacing the RPA to initiate homologous recombination by nucleating Rad51 filaments ( Krejci et al., 2012 ). Thus, Rad52 recruitment immediately upstream of the site of fork collapse is strongly indicative of DNA processing...

... homologous recombination repair (HRR) proteins can access the DNA in eukaryotes during repair. However, it remains unresolved how RNF20 itself approaches the DNA in the presence of chromatin structure. Here, we identified the histone chaperone FACT as a key protein in the early steps of HRR. Depletion...

... and hSSB1, INTS6 relocates to the DNA damage sites. Moreover, the hSSB1–INTS complex regulates the accumulation of RAD51 and BRCA1 at DNA damage sites and the correlated homologous recombination. * Author for correspondence ( [email protected] ) 15 07 2013 © 2013. Published by The Company...

...Fan Zhang; Gregory Bick; Jung-Young Park; Paul R. Andreassen Summary The PALB2 protein is associated with breast cancer susceptibility and Fanconi anemia. Notably, PALB2 is also required for DNA repair by homologous recombination (HR). However, the mechanisms that regulate PALB2, and the functional...

... on the spread of drug resistance in Giardia populations. Fig. 6. Hypothetical model of homologous recombination during diplomixis. ( A ) Shown is a simplified cell containing an integrated marker (red) in one of its four homologous chromosomes (H1 and H2, shown in orange, in the left nucleus, and H1...

...Julian E. Sale Cells must overcome replication blocks that might otherwise lead to genomic instability or cell death. Classical genetic experiments have identified a series of mechanisms that cells use to replicate damaged DNA: translesion synthesis, template switching and homologous recombination...

...Duen-Wei Hsu; Rhian Kiely; C. Anne-Marie Couto; Hong-Yu Wang; Jessica J. R. Hudson; Christine Borer; Catherine J. Pears; Nicholas D. Lakin DNA double-strand breaks (DSBs) can be repaired by homologous recombination (HR) or non-homologous end joining (NHEJ). The mechanisms that govern whether a DSB...

... 2011. 2011 DSB repair Meiosis Crossover control Homologous recombination Meiosis is the specialised reductive division that generates haploid cells. During this process, a single round of replication is followed by two rounds of chromosome segregation: in the first division (meiosis I...

... as DNA-SCARS. Because we irradiated proliferating cells, we hypothesized that cells irradiated during S phase incorporated BrdU into foci owing to homologous recombination repair (HRR), the preferred mode of DSB repair during S phase. Indeed, immunostaining revealed the presence of RPA70, a single...

... or their repair through homologous recombination but is required for crossover formation. However, RAD-51 foci in ife-2 mutants persist into inappropriately late stages of meiotic prophase at 25°C, similar to mutants defective in MSH-4/HIM-14 and MSH-5, which stabilize a critical intermediate in crossover...

... The Company of Biologists Limited 2009 2009 Homologous recombination Fluorescence recovery after photobleaching RAD51 RecQ helicase Bloom helicase (BLM) is a 1417 amino acid 3′ to 5′ helicase whose mutation leads to an autosomal recessive disorder called Bloom syndrome (BS). Patients with BS...

... the impact of homologous recombination (HR) on the velocity of DNA replication forks. We used three hamster cell lines defective in HR either by overexpression of a RAD51 dominant-negative form, or by a defect in the RAD51 paralogue XRCC2 or the breast tumor suppressor BRCA2 . Irrespectively of the type...

... in the DNA damage response ( Fig. 3 ). It might, by contrast, be reasonable to posit that the role of BRCA2 in control of the RAD51 recombinase, and through it in DNA repair by homologous recombination, constitutes a key function. This view will also probably prove simplistic if current circumstantial...

... deficiency Homologous recombination FAS/APO-1/CD95 Knockout Caspases are a family of cysteine proteases that cleave target proteins at specific aspartate residues ( Alnemri et al., 1996 ; Nicholson et al., 1995 ). In general, three amino acid residues that lie upstream of the aspartate residue...

... 1999 © 1999 by Company of Biologists 1999 Homologous recombination Phenotype Extracellular matrix In the early 1980s several labs independently discovered and characterized the glycoprotein that eventually became known as tenascin. Immunohistochemistry revealed tenascin...

... biological processes such as growth, differentiation, migration, and cell death. Of more than 20 known integrins, 10 contain the nearly ubiquitously expressed β1 integrin subunit. Disruption of the β1 integrin gene by homologous recombination allows us to assess the supposed functions of β1 containing...