..., it is not clear how chemical and mechanical signals are integrated to regulate EZH2 and gene expression. We show that culture of cells on stiff matrices in concert with transforming growth factor (TGF)-β1 promotes nuclear localization of EZH2 and an increase in the levels of the corresponding histone modification...

... and nutritional state of cells can influence these marks. These connections between the balance of metabolites, histone modifications and downstream transcriptional changes comprise a metabolic signaling program that can enable cells to adapt to changes in nutrient availability. Beyond acetylation...

... attributed. Highlighted Article: The human oncoprotein DEK forms nuclear bodies that are regulated by the SUMO pathway and localize to active sites of constitutive heterochromatin replication. Oncogene Breast cancer Replication stress Histone modification Superresolution microscopy siRNA...

... in the underlying histone modification pattern, such as during cancer development or ageing, influence the post-transcriptional life of an mRNA? The rationale for this study comes from observations in mutants affecting acetylation of histone H3K56. Acetylation at this site is important to allow access to chromatin...

... stimuli. Levels of histone modifications fluctuate during the cell cycle and vary among different cell types. Here, we describe a simple system to monitor the levels of multiple histone modifications in single cells by multicolor immunofluorescence using directly labeled modification-specific antibodies...

... element in neuron-like cell culture. Herpesvirus Thyroid hormone Differentiation Transcription factor Histone modification National Institute of Neurological Disorders and Stroke 10.13039/100000065 R01NS081109 Thyroid hormones [THs; thyroxine (T 4 ) and triiodothyronine (T...

... for the NE, denoted mNE; Fig. 1 D; for details, see Materials and Methods]. The fluorescence intensity of the respective histone modification [or other proteins of interest such as lamin B1 or HP1a (also known as CBX5)] was collected and normalized (see Materials and Methods), and the median fluorescence...

... reprogramming and increasing the efficiency of iPSC generation. We demonstrate a new histone chaperone (APLF)–MET–histone modification cohort that functions in the induction of pluripotency in fibroblasts. This regulatory axis might provide new mechanistic insights into perspectives of epigenetic regulation...

... been ambiguous. In the present study, we performed immunocytological analyses of Oryza sativa pollen mother cells (PMCs) in the mel1 mutant, and investigated the possibility of MEL1 being involved in the global control of histone modifications in meiotic chromosomes, prior to homologous...

... for nuclear pore complex assembly . Cell   113 , 195 - 206 . 10.1016/S0092-8674(03)00235-6 Wang , F. and Higgins , J. M. G. ( 2013 ). Histone modifications and mitosis: countermarks, landmarks, and bookmarks . Trends Cell Biol.   23 , 175 - 184 . 10.1016/j.tcb.2012.11.005 Wang...

... ZMPSTE24 Prelamin A accumulation miRNA-141-3p Histone modification Aging Advancing age is a major risk factor for developing many chronic diseases, but the mechanisms that regulate the aging process remain largely unknown. Recent studies have revealed that aging has been negatively correlated...

[email protected] ) 13 11 2012 © 2013. Published by The Company of Biologists Ltd 2013 ErbB4 Nuclear translocation EGFR Src-family tyrosine kinases Histone modification Protein-tyrosine kinases are found in multicellular organisms and have important roles in a variety...

.... Published by The Company of Biologists Ltd 2012 Histone modification H2A.Z Single molecule Fluorescence correlation spectroscopy (FCS) The simplest structural unit of chromatin, the nucleosome, can exist in a variety of configurations depending on the histone variants and post-translational...

... in the regulation of histone H1.4 mobility and chromatin binding in mitosis. * Author for correspondence ( [email protected] ) 24 1 2011 © 2011. 2011 Aurora B Histone H1 Histone modification Phosphorylation Together with the nucleosomal core particle, the linker histone...

....ibp.ac.cn ) 20 5 2010 © 2010. 2010 Drosophila CAF-1 p180 Heterochromatin Histone modification Position effect variegation In eukaryotic cells, DNA is packaged in repeated units of nucleosomes, which are further organized into chromatin, a template for gene expression...

... in decondensation of the male-specific chromocenter that normally arises from the developmentally-regulated facultative heterochromatinisation of the paternal chromosome complement. Together with the disappearance of the chromocenter the staining levels of two associated histone modifications, tri -methylated...

... for destruction. * Author for correspondence (e-mail: [email protected] ) 29 6 2006 © The Company of Biologists Limited 2006 2006 Ask1 Nucleolus Meiosis Ribosomal Dna Histone Modification Meiosis is a central feature in the sexual reproduction of all eukaryotic organisms...

... via histone modifications, plays a crucial role in regulating gene expression. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are two groups of enzymes with opposing activities and are the main enzymes responsible for histone modification by modulating their acetylation status...