... and male sterility. Immunofluorescence and fluorescent in situ hybridization revealed that Ipo9 KO mutants display chromosome condensation and segregation defects during meiosis. In addition, Ipo9 KO mutant males form abnormally structured sperm and fail to properly exchange histones for protamines. Ipo9...

... the real-time addition of drugs and fluorescent probes to one or more nuclei while under microscopy observation. A high-resolution map of 11 endogenous nuclear markers of the histone code, transcription machinery and architecture was obtained in transcriptionally active nuclei by performing confocal...

... cycle, replication-independent assembly is the only mode available for chromatin remodeling. For this reason, any nucleosome composition alterations accompanying transcriptional responses to physiological signals must occur through a DNA replication-independent pathway. HIRA is the histone chaperone...

...Shinichi Kadota; Kyosuke Nagata Type I interferon (IFN) plays a crucial role in establishing the cellular antiviral state by inducing transcription of IFN-stimulated genes (ISGs). Generally, histone acetyltransferases (HATs) are positive regulators of transcription, but histone deacetylase (HDAC...

...Floor Frederiks; Tibor van Welsem; Gideon Oudgenoeg; Albert J. R. Heck; Christian J. Janzen; Fred van Leeuwen Dot1 is a highly conserved methyltransferase that modifies histone H3 on the nucleosome core surface. In contrast to yeast, flies, and humans where a single Dot1 enzyme is responsible...

... of Drosophila melanogaster lamin Dm 0 , which binds chromatin in vitro via the histone H2A/H2B dimer. This region contains an evolutionarily conserved nuclear localization signal (NLS) KRKR, and a sequence composed of the amino acids TRAT. Here we show that binding of lamin Dm 0 to chromatin requires both NLS...

...Elana Hariton-Gazal; Joseph Rosenbluh; Adolf Graessmann; Chaim Gilon; Abraham Loyter The present work shows that histones are able to directly cross cell plasma membranes and mediate penetration of macromolecules covalently attached to them. Adding a mixture containing the five nucleosomal histones...

... with multiple proteins. Using co-fractionation and co-immunoprecipitation we demonstrate that hDaxx associates with proteins that are critical for transcriptional repression, such as histone deacetylase II, constituents of chromatin such as core histones H2A, H2B, H3 and H4, and Dek, a chromatin-associated...

...Samantha G. Zeitlin; Cynthia M. Barber; C. David Allis; Kevin E. Sullivan ABSTRACT After DNA replication, cells condense their chromosomes in order to segregate them during mitosis. The condensation process as well as subsequent segregation requires phosphorylation of histone H3 at serine 10...

... difficult. Differentiation involves the assembly of specialized forms of repressive chromatin including linker histones, Polycomb group proteins and methyl-CpG-binding proteins. These structures compartmentalize chromatin into functional domains and maintain the stability of the differentiated state through...

...Aaron Van Hooser; David W. Goodrich; C. David Allis; B. R. Brinkley; Michael A. Mancini ABSTRACT The temporal and spatial patterns of histone H3 phosphorylation implicate a specific role for this modification in mammalian chromosome condensation. Cells arrest in late G 2 when H3 phosphorylation...

... origins. Since this repression can be relieved by either butyrate or enhancers, it appears to be mediated through chromatin structure. Here we identify changes in the synthesis and modification of chromatin bound histones that are consistent with this hypothesis. Oocytes, which can repress promoter...

...M. Grunstein; A. Hecht; G. Fisher-Adams; J. Wan; R. K. Mann; S. Strahl-Bolsinger; T. Laroche; S. Gasser ABSTRACT Yeast chromosomes may lack the linker histone Hl (normally required to compact 10 nm beads-on-a-string fiber into the 30 nm fiber) and there is no cytological evidence for higher order...

...Alison L. Symington; Selma Zimmerman; Janet Stein; Gary Stein; Arthur M. Zimmerman ABSTRACT Exposure of HeLa S3 cells to high hydrostatic pressure (6.89 ×10 3 to 6.89 ×10 4 kPa: 1000 to 10000lbfin -2 ) reduced core and H1 histone mRNA levels as determined by hybridization to specific histone DNA...

...Mathias G. Bergman; Edgar Wawra; MÅrten Winge ABSTRACT Chicken erythrocyte histone H5 has been suggested repeatedly to be a general suppressor of transcription and replication. Therefore, the biological functions of H5 were investigated and compared with those of Hl (Hla + Hlb) by microinjection...