... is seen in c1da neurons, augmin expression is increased in c4da neurons and is required in these neurons for the growth of fine dendritic branches. In the rodent hippocampal neurons in culture, as well as in Drosophila c4da neurons in vivo , absence of augmin affects MT polarity in the axon...

... hippocampal neurons from Sh2b1 −/− [knockout (KO)] mice exhibit decreased neurite complexity and length, and BDNF-induced expression of the synapse-related immediate early genes Egr1 and Arc . Reintroduction of each SH2B1 isoform into KO neurons increases neurite complexity; the brain-specific δ isoform also...

... hippocampal neurons in culture, we showed that LGI1 is recruited to the AIS where it colocalizes with ADAM22 and Kv1 channels. Strikingly, the missense mutations S473L and R474Q of LGI1 identified in ADLTE prevent its association with ADAM22 and enrichment at the AIS. Moreover, we observed that ADAM22...

...Tam T. Quach; Sarah M. Wilson; Veronique Rogemond; Naura Chounlamountri; Pappachan E. Kolattukudy; Stephanie Martinez; May Khanna; Marie-Francoise Belin; Rajesh Khanna; Jerome Honnorat; Anne-Marie Duchemin Summary Although hippocampal neurons are well-distinguished by the morphological...

... remain largely uncharacterized. We recently showed that Shh and its signaling components, Patched and Smoothened, are expressed in postnatal and adult hippocampal neurons. We have now examined whether Shh signaling has a function in these neurons. Using cultured hippocampal neurons as a model system, we...

..., but it is not fully understood how channels are localized to the soma. We have now identified a motif within the calcium-activated potassium channel K Ca 2.1 (SK1) that results in somatic localization. Transfection of hippocampal neurons with K Ca 2.1 subunits causes expression of functional channels in only the soma...

..., but not PITPβ or a PITPα mutant deficient in binding PtdIns, enhances laminin-dependent extension of axonal processes in hippocampal neurons, whereas knockdown of PITPα protein by siRNA suppresses laminin and BDNF-induced axonal growth. PITPα-mediated axonal outgrowth is sensitive to phosphoinositide 3-kinase...

... by producing a series of enhanced green fluorescent protein (eGFP)-tagged MKLP1 and its variant mutations, and studying the distribution of these molecules in cultured primary hippocampal neurons using fluorescence microscopy. We have found that: (a) full-length MKLP1(1-856)-eGFP was distributed...

...Carlos G. Dotti; Gary Banker ABSTRACT In culture, hippocampal neurons initially establish several short, apparently identical processes; of these, only one acquires axonal characteristics, the remainder becoming dendrites. We examined the organization of cytoplasmic constituents that might...