..., there is no difference in the amino acid sequence of the protein. The N-terminus of GS, which constitutes a ‘weak’ mitochondrial targeting signal (MTS), is sufficient to direct a chimeric protein to the mitochondria in hepatocytes and to the cytoplasm in astrocytes. Considering that a weak MTS is dependent on a highly...

... or pathological cell division programs leading to tetraploid progenies. In this study we decipher a new mechanism of incomplete cytokinesis taking place in hepatocytes during post-natal liver growth. This process is initiated in vivo after weaning and is associated with an absence of anaphase cell elongation...

.... To address this question, we employed immortalized murine hepatocytes, which display a high degree of differentiation and, expectedly, arrest in the G1 phase under exposure to TGF-β1. These hepatocytes maintain epithelial polarization upon expression of oncogenic Ha-Ras. However, Ras-transformed hepatocytes...

... of cytokeratin (CK) 8-deficient mice, which comprises only colorectal hyperplasia, female sterility and a weaker hepatocyte integrity. In this work localization with anti-CK antibodies indicated that many Ck8 − / − epithelia still form IF in CK8-deficient mice, perhaps because of the expression...

...G. Flouriot; C. Vaillant; G. Salbert; C. Pelissero; J. M. Guiraud; Y. Valotaire ABSTRACT An aggregate culture system for rainbow trout hepatocytes was developed to study liver-specific mRNA expression. Maintenance of differentiated functions and morphology of hepatocytes were examined using both...

...M. Douaire; B. Belloir; J.-C. Guillemot; J.-M. Fraslin; P. Langlois; J. Mallard ABSTRACT Hepatocytes isolated from 9-week-old chickens were cultured in a serum-free, hormonally defined medium. Relative amounts of mRNAs coding for lipogenic enzymes (acetyl-CoA carboxylase, fatty acid synthase, 9...

...Georges Baffet; Pascal Loyer; Denise Glaise; Anne Corlu; Pierre-Luc Etienne; Christiane Guguen-Guillouzo ABSTRACT Cytokeratins CK 8 and CK 18 are the two keratins expressed in the liver. They are known to undergo extensive changes in expression with alteration of the hepatocyte phenotype in vitro...

...James R. Bartles; M. Sambasiva Rao; Liqin Zhang; Barbara E. Fayos; Cheryl L. Nehme; Janardan K. Reddy ABSTRACT A combination of Western blotting, Northern blotting and immunofluorescence was used to examine the expression and compartmentalization of plasma membrane proteins by those hepatocyte-like...

...Stamatis C. Stamatoglou; Kate H. Sullivan; Staff An Johansson; Peter M. Bayley; Ian D. J. Burdett; R. Colin Hughes ABSTRACT We have compared the localization of integrin α 5 β 1 and AGp100 (apical glycoprotein of M r 110×10 3 ) in rat liver parenchyma and in primary hepatocyte cultures. Integrin α...