... nonalcoholic fatty liver disease (NAFLD). In this study, we found that inflammatory macrophages cause injury to hepatocytes, in part by a cell–cell crosstalk phenomenon involving the secretion of EVs containing pro-inflammatory cargo. Incorporation of these inflammatory signals into EV requires the cleavage...

...Stéphane Gilbert; Anne Loranger; M. Bishr Omary; Normand Marceau ABSTRACT Keratins are epithelial cell intermediate filament (IF) proteins that are expressed as pairs in a cell-differentiation-regulated manner. Hepatocytes express the keratin 8 and 18 pair (denoted K8/K18) of IFs, and a loss of K8...

...Arnab Gupta; Michael J. Schell; Ashima Bhattacharjee; Svetlana Lutsenko; Ann L. Hubbard ABSTRACT The cellular machinery responsible for Cu + -stimulated delivery of the Wilson-disease-associated protein ATP7B to the apical domain of hepatocytes is poorly understood. We demonstrate that myosin Vb...

...Ting Wang; Kilangsungla Yanger; Ben Z. Stanger; Doris Cassio; Erfei Bi ABSTRACT By definition, all epithelial cells have apical–basal polarity, but it is unclear how epithelial polarity is acquired and how polarized cells engage in tube formation. Here, we show that hepatocyte polarization...

...-APs associate with rafts in the trans-Golgi network and are directly delivered to the apical membrane. It has been shown that oligomerization is required for their stabilization in rafts and their apical targeting. In hepatocytes, GPI-APs are first delivered to the basolateral membrane and secondarily...

... and post-natal tissue homeostasis. Gene expression and function is therefore tightly controlled at a number of levels. We investigated the role that post-translational modifications play during human hepatocyte differentiation. In particular, we examined the role of the small ubiquitin-like modifier (SUMO...

...Delphine Haouzi; Stephen Baghdiguian; Guillaume Granier; Pierre Travo; Paul Mangeat; Urszula Hibner Maintenance of epithelial cell shape and polarity determines many vital cell functions, including the appropriate response to external stimuli. Murine hepatocytes cultured in a three-dimensional...

... + hepatocytes and cytokeratin 19 + (CK19) cholangiocytic cells on either type IV collagen or laminin. Here we show that extracellular matrices (ECMs) significantly affect the growth of Dlk + cells. Dlk + cells vigorously proliferated on type IV collagen-coated dishes in the presence of EGF and HGF during...

...Naoki Tanimizu; Atsushi Miyajima Hepatoblasts give rise to both mature hepatocytes and cholangiocytes. While Notch signaling has been implicated in the formation of bile ducts composed of cholangiocytes, little is known about the mechanism of lineage commitment of hepatoblasts. Here we describe...

...Naoki Tanimizu; Mitsuo Nishikawa; Hiroki Saito; Tohru Tsujimura; Atsushi Miyajima Hepatoblasts are common progenitors for hepatocytes and biliary epithelial cells, although their nature remains largely unknown. In order to isolate and to characterize hepatoblasts, we searched for cell surface...

...Giridhar R. Gorla; Harmeet Malhi; Sanjeev Gupta Polyploid cells are encountered ubiquitously but the biological significance of polyploidy is unclear. In view of their extensive capacity for regeneration, hepatocytes offer excellent systems for analyzing growth control mechanisms. We isolated...

... cell line. Similar to hepatocytes, WIF-B cells secrete bile acids and organic cations (i.e. rhodamine-123) into the bile canaliculi. Canalicular secretion of fluorescein isothiocyanate-glycocholate was stimulated by taurocholate and a decapeptide activator of phosphoinositide 3-kinase and was decreased...

...Linda K. Hansen; Jeffrey H. Albrecht ABSTRACT Rat hepatocytes adherent to a rigid film of type I collagen will spread and enter S phase, while those attached to collagen gel or a dried collagen substrate remain round and quiescent. The current studies were initiated to determine the mechanism...

... organic anion transport into bile. The physiological function of MRP1 in hepatocytes is unknown. Previous results show that MRP1 expression is low in quiescent hepatocytes but increased after SV40 large T antigen immortalization, suggesting a relationship with cell proliferation. Therefore, we determined...

...Han Roelofsen; Carol J. Soroka; Dietrich Keppler; James L. Boyer ABSTRACT The canalicular membrane of rat hepatocytes contains an ATP-dependent multispecific organic anion transporter, also named multidrug resistance protein 2, that is responsible for the biliary secretion of several amphiphilic...

... are defined here in terms of alterations in projected cell areas, as determined by computerized image analysis. Cell spreading kinetics and changes in microtubule and actin microfilament mass were simultaneously quantitated in hepatocytes plated on different extracellular matrix substrata. The initial rate...

...Manuel Portolés; Magdalena Faura; Jaime Renau-Piqueras; Francisco José Iborra; Rosana Saez; Consuelo Guerri; Joan Serratosa; Eulalia Rius; Oriol Bachs ABSTRACT We report here that a 62 kDa calmodulin-binding protein (p62), recently identified in the nucleus of rat hepatocytes, neurons and glial...

...Gregg D. Simonson; Alexandra C. Vincent; Kevin J. Roberg; Yue Huang; Victoria Iwanij ABSTRACT Monoclonal antibodies that specifically recognize a membrane component located on the sinusoidal domain of the hepatocyte have been used to screen a rat liver cDNA expression library and a clone encoding...

... cystathionine synthase gene activation hepatocyte ornithine transcarbamylase phenylalanine hydroxylase sparse-fur mice. The mouse hepatoma, BWTG3, is moderately differentiated. It is known to produce several plasma proteins, including a-foetoprotein and albumin ( Szpirer and Szpirer, 1975...

...M. Maurice; E. Rogier; D. Cassio; G. Feldmann ABSTRACT In vivo , proteins of the hepatocyte plasma membrane are asymmetrically distributed, making it possible to distinguish a sinusoidal, a lateral and a canalicular domain. The conditions that determine hepatocyte plasma membrane polarity have been...