...Laura Virtanen; Emilia Holm; Mona Halme; Gun West; Fanny Lindholm; Josef Gullmets; Juho Irjala; Tiina Heliö; Artur Padzik; Annika Meinander; John E. Eriksson; Pekka Taimen ABSTRACT The heat shock (HS) response is crucial for cell survival in harmful environments. Nuclear lamin A/C, encoded...

...Katharina S. Keuenhof; Lisa Larsson Berglund; Sandra Malmgren Hill; Kara L. Schneider; Per O. Widlund; Thomas Nyström; Johanna L. Höög ABSTRACT When the temperature is increased, the heat-shock response is activated to protect the cellular environment. The transcriptomics and proteomics...

... complexes. Although metazoan SGs are dynamic compartments where proteins can rapidly exchange with their surroundings, yeast SGs seem largely static. To gain a better understanding of yeast SGs, we identified proteins that sediment after heat shock using mass spectrometry. Proteins that sediment upon heat...

...Roopali Pradhan; Muhunden Jayakrishnan Nallappa; Kundan Sengupta ABSTRACT The structure–function relationship of the nucleus is tightly regulated, especially during heat shock. Typically, heat shock activates molecular chaperones that prevent protein misfolding and preserve genome integrity...

..., 1:5000). Secondary antibodies used were: goat anti-rabbit IgG-HRP (Abmart M21002L, 1:5000) and goat anti-mouse IgG-HRP (Abmart M21001L, 1:5000). Zebrafish at different ages were heat-shocked for 20 min, then fixed in fresh 4% paraformaldehyde in PBS overnight at 4°C and dehydrated using...

... to arsenite and ionising radiation, but decreases in response to heat shock or hippuristanol. In arsenite-treated cells, but not in hippuristanol-treated cells, eIF4A2 is recruited to stress granules, suggesting sumoylation of eIF4A2 correlates with its recruitment to stress granules. Furthermore, we...

... by resorption. In mammalian cells loss of cilia correlated with a reduction in hedgehog signalling. Heat-shock-dependent loss of cilia was decreased in cells where histone deacetylases (HDACs) were inhibited, suggesting resorption is mediated by the axoneme-localised tubulin deacetylase HDAC6. In thermotolerant...

... and can facilitate insulator complex formation, but are nonessential for insulator action. * Authors for correspondence ( [email protected] ; [email protected] ) 12 12 2011 © 2012. 2012 Chromatin insulator CP190 Heat shock Nuclear speckles Sumoylation Funding...

...Mathieu Nivon; Michel Abou-Samra; Emma Richet; Boris Guyot; André-Patrick Arrigo; Carole Kretz-Remy We previously found that the NF-κB transcription factor is activated during the recovery period after heat shock; moreover, we demonstrated that NF-κB is essential for cell survival after heat shock...

... expression. We found that in heat-shocked cells, the actin-severing protein cofilin acquires inhibitory Ser3 phosphorylation, which is associated with an inhibition of chemokine-stimulated cell migration. Cofilin phosphorylation appeared to occur as a result of the heat-induced insolubilization...

...Susanne Kramer; Rafael Queiroz; Louise Ellis; Helena Webb; Jörg D. Hoheisel; Christine Clayton; Mark Carrington In trypanosomes there is an almost total reliance on post-transcriptional mechanisms to alter gene expression; here, heat shock was used to investigate the response to an environmental...

...Xian-Chun Zeng; Samir Bhasin; Xufeng Wu; Joeng-Goo Lee; Shivani Maffi; Christopher J. Nichols; Kyung Jin Lee; J. Paul Taylor; Lois E. Greene; Evan Eisenberg The molecular chaperone Hsp70 interacts with misfolded proteins and also accumulates in the nucleus during heat shock. Using GFP-Hsp70...

...Alexandra Metz; Johann Soret; Claire Vourc'h; Jamal Tazi; Caroline Jolly Exposure of cells to stressful conditions results in the rapid synthesis of a subset of specialized proteins termed heat shock proteins (HSPs) which function in protecting the cell against damage. The stress-induced activation...

... heat shock Daxx and Sp100 were released but PML remained, whereas exposure to subtoxic concentrations of CdCl 2 induced the release of ND10-associated proteins, including PML, with Sp100 remaining in a few sites. In both cases,recovery times were similar and were followed by a burst of mitotic activity...

... monitored for integrity and composition, and then sorted for productive translational initiation or targeted degradation. References Arrigo, A. P., Suhan, J. P. and Welch, W. J. ( 1988 ). Dynamic changes in the structure and intracellular locale of the mammalian low-molecular-weight heat shock protein...

... coexpressing cells( Yamauchi et al., 2001 ). We found that the protein shared some characteristics of heat shock proteins(HSPs) or molecular chaperones, such as nuclear translocation upon heat shock,ATP deprivation and osmotic shock. Interestingly, a significant homology over a stretch of 15 amino acids...

... Cajal bodies with diameters up to 10 μm. In this study we show that numerous mini-Cajal bodies (less than 2 μm in diameter) form in the germinal vesicle after oocytes recover from heat shock. The mechanism for heat shock induction of mini-Cajal bodies is independent of U7 snRNA and does not require...

..., a transcriptional regulator of the gene for heat shock protein 27. After heat shock, HAP is recruited to a few nuclear bodies. Here we report the characterisation of these bodies, which are distinct from other nuclear components such as coiled bodies and speckles. The formation of HAP bodies is part of a general...

... colocalization of the hnRNPs and the omega speckles. Heat shock caused all the hnRNPs to cluster together exactly, following the hsrω-n transcripts. Immunoprecipitation studies using the hnRNP antibodies further demonstrated a physical association of hnRNPs and hsrω transcripts. The omega speckles are distinct...

...Philippe A. Mercier; Neil A. Winegarden; J. Timothy Westwood ABSTRACT The induction of the heat shock genes in eukaryotes by heat and other forms of stress is mediated by a transcription factor known as heat shock factor 1 (HSF1). HSF1 is present in unstressed metazoan cells as a monomer with low...