... samples in 3D. Using GFP-tagged GLUT4 as proof of concept, we present here a novel optical mesoscopy approach that allows precise measurement of the spatial location of GLUT4 within specific anatomical structures across the myocardium in ultrathick sections (5 mm×5 mm×3 mm) of intact mouse heart. We...

.... These minor introns are present in certain gene families with specific functions, such as voltage-gated Na + and voltage-gated Ca 2+ channels. Removal of minor introns by the minor spliceosome has been proposed as a post-transcriptional regulatory layer, which remains unexplored in the heart. Here, we...

...-206 deletion shifts slow muscles to a faster profile and results in cardiac dysfunction specifically in male mice. miR-206 miRNA Skeletal muscle Heart Sexual dimorphism National Institutes of Health 10.13039/100000002 GM29090 2T32HL007822-11A2 5T32GM007135-36 K01AR055676...

... therapeutic target in promoting recovery from injury. Zebrafish CDK9 LARP7 Heart Repair Cyclin dependent kinase 9 (CDK9) has emerged from the wider family of CDKs as an important signaling mechanism in the initiation and progression of cardiac hypertrophy ( Krystof et al., 2012 , 2010...

... (Cx43, also known as GJA1), cadherin and actin. Vcl and ZO-1 anchor the actin cytoskeleton to the sarcolemma. Given that loss of Vcl from cardiomyocytes causes maldistribution of Cx43 and predisposes cardiomyocyte-specific Vcl-knockout mice with preserved heart function to arrhythmia and sudden death...

... expression, thereby relieving the inhibitory influence of Zeb2 on Meox2 expression levels. In the damaged heart, however, that ‘brake’ might be released by the shuttling of Ski from the nucleus to the cytosol. Understanding the specific mechanisms that orchestrate fibroblast-to-myofibroblast...

...) mice, which form no cilia, have ventricular dilation, decreased myocardial trabeculation and abnormal outflow tract development. These data support the conclusion that cardiac primary cilia are crucial in early heart development, where they partly coordinate hedgehog signaling. References Angello...

.... Horne-Badovinac, S., Lin, D., Waldron, S., Schwarz, M., Mbamalu, G., Pawson, T., Jan, Y., Stainier, D. Y. and Abdelilah-Seyfried, S. ( 2001 ). Positional cloning of heart and soul reveals multiple roles for PKC lambda in zebrafish organogenesis. Curr. Biol. 11 , 1492 -1502. Hough, C. D...

... microscopy, that desmosomal proteins in the heart localize not only to the desmosomes in the intercalated discs but also at adhering junctions with hybrid composition. We found that in the latter junctions, endogenous plakophilin-2 colocalizes with αT-catenin. By providing an extra link between the cadherin...

... expression and organization during embryonic stem cell differentiation towards cardiomyocyte. We show that the sequence of actin isoform expression displays similar pattern in the in vitro model and in mouse heart embryogenesis. Treatment with an interfering fusion peptide containing the N-terminal sequence...

... that labeling of this form of Cx43 as well as of total Cx43 is restricted to the intercalated disk region of normal ventricular tissue. In ischemic heart, significant relocalization of total Cx43 to the lateral edges of myocytes was evident; however pS325/328/330-Cx43 remained predominately at the intercalated...

...Preeti Ahuja; Evelyne Perriard; Jean-Claude Perriard; Elisabeth Ehler The contractile tissue of the heart is composed of individual cardiomyocytes. During mammalian embryonic development, heart growth is achieved by cell division while at the same time the heart is already exerting its essential...

...Mónica Bettencourt-Dias; Sybille Mittnacht; Jeremy P. Brockes Adult newt cardiomyocytes, in contrast to their mammalian counterparts, can proliferate after injury and contribute to the functional regeneration of the heart. In order to understand the mechanisms underlying this plasticity we...

... 2003 Nitric oxide Heart Protein targeting PDZ domain The free radical gas nitric oxide (NO) is now recognized as a major messenger molecule that has diverse functions throughout the body. Although originally identified as the endothelial-derived relaxing factor( Furchgott and Zawadzki...

..., mAKAP may serve as the scaffold for a cAMP- and calcium ion-sensitive signaling complex. *Author for correspondence (e-mail: [email protected] ) 25 5 2001 © The Company of Biologists Limited 2001 2001 mAKAP Protein kinase A cAMP Ryanodine receptor Nuclear envelope Heart...

... cDNA encoding a protein with homology to both human αE(epithelial)-catenin and αN(neural)-catenin. Although originally discovered in testis, αT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human αT-catenin localization...

... kDa polypeptides were detected. In rat heart and skeletal muscle, both long and short C-terminal forms of βII spectrin localized in the region of the Z line. The central region of the sarcomere, coincident with the M line, was selectively labeled with antibodies to the short C-terminal form...

... intracellular locations through association with A-kinase anchoring proteins (AKAPs). We now report the cloning of mAKAP, an anchoring protein found predominantly in heart, skeletal muscle and brain, and whose expression is induced in neonatal ventriculocytes by treatment with hypertrophic stimuli. mAKAP...

...Robert G. Gourdie; Nicholas J. Severs; Colin R. Green; Stephen Rothery; Patricia Germroth; Robert P. Thompson ABSTRACT Electrical coupling between heart muscle cells is mediated by specialised regions of sarcolemmal interaction termed gap junctions. In previous work, we have demonstrated...

... propagation, in the mammalian heart. Optical section series obtained by laser scanning confocal microscopy permitted the quantification and mapping of the three-dimensional distribution of gap junctions in ventricular intercalated disks with high clarity over substantial specimen depths. A consistent feature...