...Francine Puvion-Dutilleul; Jean-Pierre Bachellerie; Neus Visa; Edmond Puvion ABSTRACT We have studied in HeLa cells at the electron microscope level the response to adenovirus infection of the RNA pro- cessing machinery. Components of the spliceosomes were localized by in situ hybridization...

...Sibdas Ghosh; Neidhard Paweletz; Dieter Schroeter ABSTRACT Mitotic HeLa cells were treated with different concentrations of okadaic acid (OA), known to inhibit phosphatase 1 and 2A activities. The cytological effects on the course of mitosis were studied at the light microscopic, immunofluorescence...

...Sergio Schenkman; Renato A. Mortara ABSTRACT We show here that HeLa cell microfilaments can be stained by phalloidin at the sites of invasion of Trypanosoma cruzi trypomastigotes, one of the infective stages of this protozoan parasite. Concurrently, a projection of the HeLa cell plasmalemma...

... of dividing onion root cells and human HeLa cells. Our observations disagree considerably with conclusions drawn from work with cell-free systems. We consistently observed reassembly of nuclear envelope cisternae from vesicles in the cytoplasm without direct contact with chromatin. The preassembled envelope...

... 1991 Hela cells nuclear matrix The nuclear matrix is defined as the salt-resistant proteinaceous nuclear structure that is isolated from the interphase cell ( Berezney and Coffey, 1974 , 1977 ; Shaper et al . 1979 ; Agutter and Richardson, 1980 ; Bouteille et al . 1983 ; Hancock...

...Srijata Sarkar; Nicholas J. Cowan ABSTRACT The regulation of cell type-specific expression of the gene encoding glial filament acidic protein (GFAP) was examined by introducing various deletion mutants of the gene into GFAP-expressing (U251 human astrocytoma) and non-expressing (HeLa) cell lines...

...M. Lourdes Monteiro Da Silva; Renato A. Mortara; Helena C. Barros; Wanderley De Souza; Luiz R. Trabulsi ABSTRACT We have previously observed that enteropathogenic Escherichia coli (EPEC) adhere to HeLa cells in a localized manner, which we designated localized adherence as opposed to the diffuse...

.... It was demonstrated that the cloned desmin gene was expressed after gene transfer and that the newly synthesized protein assembles into intermediate filaments. Here we present data on the transfection of modified vimentin and desmin genes onto simian virus 40-transformed hamster lens cells and HeLa cells...

... cytosolic calcium ions. In this paper we examine the effects of two agents known to perturb intracellular calcium pools on mitosis in HeLa cells. These were the calcium-selective ionophore A23187 and carbonyl cyanide n-chlorophenylhydrazone (CCCP), which is a protonophoric inhibitor of oxidative...

... vaccinia virus HeLa cells X-ray microanalysis Na K and Cl concentrations X-ray microanalysis has been used to study patho10gical material originally prepared and examined by conventional electron microscopy (reviewed by Ghadially, 1979; Moreton, 1981 ). Such studies allow detection of heavy...