... as described above and starved overnight in DMEM supplemented with 0.2% BSA and 0.5% FBS. PDGF-BB (0.5 nM) and 70 kDa TMR–dextran (1 mg/ml) were added to the cells for 30 min at 37°C. MDA-MB-231 cells were seeded on glass and cultured overnight in DMEM supplemented with 0.2% BSA, and then incubated with HGF...

...Simone Radtke; Mina Milanovic; Carine Rossé; Manu De Rycker; Sylvie Lachmann; Andrew Hibbert; Stéphanie Kermorgant; Peter J. Parker Summary Aberrant signalling of receptor tyrosine kinases (RTKs), such as c-Met, the receptor for hepatocyte growth factor (HGF), has been implicated in the oncogenesis...

... for correspondence ( [email protected] ) 7 11 2012 © 2013. Published by The Company of Biologists Ltd 2013 Plexin B1 Melanocyte MET HGF Hepatocyte growth factor RTK Receptor tyrosine kinases Melanocytes are highly dendritic cells derived from the neural crest...

...Jun-Kai Lai; Han-Chung Wu; Yuh-Chiang Shen; Hsin-Ying Hsieh; Shu-Yi Yang; Chia-Che Chang Summary Hepatocyte growth factor/scatter factor (HGF) is unique by inducing epithelial cell scattering, a cellular event pivotal to HGF-mediated invasive-growth response essential for embryonic development...

...Joshua J. Steffan; Brittany C. Williams; Tomas Welbourne; James A. Cardelli Hepatocyte growth factor (HGF) is found in tumor microenvironments, and interaction with its tyrosine kinase receptor Met triggers cell invasion and metastasis. It was previously shown that acidic extracellular pH...

... were stimulated with 4.5 nM or 6 nM InlB, or with 0.6 nM HGF, as indicated, for 5 minutes and fixed in 3% paraformaldehyde in PBS. Immunolabelling and ruffling quantification were performed as described ( Bierne et al., 2001 ). Cells in six-well plates were lysed in 200 μl RIPA buffer...

... the regulation of the recently identified group II PAKs(PAK4-6). Here we report that PAK4 is activated by HGF, a migratory stimulus for epithelial cells. In unstimulated MDCK cells, activated PAK4 induces a decrease in stress fibres, and when cells are stimulated with HGF, it induces a loss of focal complexes...

... such molecule. It triggers bacterial entry by interacting with the hepatocyte growth factor receptor (HGF-R or Met)and two other cellular components: gC1q-R and proteoglycans. Recent studies point to significant similarities between the molecular mechanisms underlying InlB-mediated entry into cells and classic...

... membrane, whereas the `basal' form ofα v β 3 is also present in the sealing zone. Moreover, hepatocyte growth factor (HGF) and macrophage colony stimulating factor (M-CSF), two molecules involved in osteoclastogenesis and osteoclast survival, modulate α v β 3 conformation in vitro. Preincubation with HGF...

... regulation in epithelial cells. We used HA-tagged SGK1 to transiently transfect MDCK cells and study the regulation of SGK1 upon stimulation with HGF, cAMP or upon adhesion of the cells to immobilized fibronectin. In addition, we studied the regulation of SGK1 activity by small GTP-binding proteins...

... of specific genes by activating its receptor tyrosine kinase. In this work we have isolated, characterized and used as agonists two monoclonal antibodies (mAbs) directed against the extracellular domain of HGF receptor to investigate the requirements for receptor activation and for the different biological...