... and target Rab27a. Organelle transport Guanine nucleotide exchange factor Rab27a Rab3GEP MADD Melanocyte Medical Research Council G1100063 Wellcome Trust 10.13039/100010269 091346/Z/10/Z 108429/Z/15/Z University of Nottingham 10.13039/501100000837...

...Ye Dee Tay; Marcin Leda; Andrew B. Goryachev; Kenneth E. Sawin ABSTRACT The conserved Rho-family GTPase Cdc42 plays a central role in eukaryotic cell polarity. The rod-shaped fission yeast Schizosaccharomyces pombe has two Cdc42 guanine nucleotide exchange factors (GEFs), Scd1 and Gef1, but little...

... in a high-content analysis. GTPase activation Split-GFP Protein–protein interaction GEF Guanine nucleotide exchange factor Cell-based assays High-content analysis La Ligue Régionale contre le Cancer Institut National de la Santé et de la Recherche Médicale Small...

... the conserved Mon1–Ccz1 complex as the guanine nucleotide exchange factor (GEF) of the yeast Rab7 GTPase Ypt7. To address the possible GEF mechanism, we generated a homology model of the predicted longin domains of Mon1 and Ccz1 using the Rab-binding surface of the TRAPP complex as a template. On the basis...

... is a well-characterized modulator of actomyosin contractility, but the function of other GTPases, such as Rac, in regulating contractility is currently not well understood. Here, we show that activation of Rac by the guanine nucleotide exchange factor Asef2 (also known as SPATA13) impairs migration on type...

... 05 2013 © 2013. Published by The Company of Biologists Ltd 2013 TEM4 Cell junction RhoA Guanine nucleotide exchange factor Cell adhesion Catenin The actin cytoskeleton is critical for cell–cell adhesion, cytokinesis, intracellular trafficking and cell motility ( Hall, 1998...

...-ribosylation factor Guanine nucleotide exchange factor GTPase GTPase-activating protein Trans-Golgi network The ADP-ribosylation factor (ARF) family, a subfamily of the RAS small GTPases superfamily, is composed of Arf and Arf-like (Arl) proteins and functions as a regulator of vesicular...

.... * Author for correspondence (e-mail: [email protected] ) 3 6 2008 © The Company of Biologists Limited 2008 2008 Rac1 Rac3 Actin Guanine nucleotide exchange factor Neurite differentiation Neurite differentiation is a cellular process responsible...

...Xinjun Zhang; Xi He; Xin-Yuan Fu; Zhijie Chang The small GTPases Rab5 and Rab21 are closely related, and play essential roles in endocytic trafficking. Rab5 is regulated by VPS9-domain-containing guanine nucleotide exchange factors. Here, we describe a new VPS9-domain protein with ankyrin repeats...

... for correspondence (e-mail: [email protected] ) 19 6 2003 © The Company of Biologists Limited 2003 2003 Small GTPase Rab5 RIN Amphiphysin II Endocytosis Guanine nucleotide exchange factor Rab proteins constitute a subfamily of small GTPases that play central roles...

.../HEF1 association domain also shows sequence similarity with guanine nucleotide exchange factors for Ras family small GTPases. In this study, we found significant activation of Rap1 in Chat-overexpressing cells. Myr-Chat, a membrane-targeted form of Chat,activated Rap1 more efficiently. Interestingly...

...Valérie Derrien; Carole Couillault; Michel Franco; Stéphanie Martineau; Philippe Montcourrier; Rémi Houlgatte; Philippe Chavrier We recently reported the identification of EFA6 ( e xchange f actor for A RF 6 ), a brain-specific Sec7-domain-containing guanine nucleotide exchange factor that works...

... was blocked by mM [Mg 2+ ], unless a heat- and trypsin-sensitive component present in neutrophil plasma membrane was added. Liberation of barbed ends seems therefore to be mediated by a toxin B- sensitive cytosolic Rho-family protein, requiring a membrane-associated guanine nucleotide exchange factor (GEF...