... Endoplasmic reticulum Glutathione Green fluorescent protein Reduction potential Unfolded protein response The endoplasmic reticulum (ER) constitutes the starting point of the secretory pathway where secretory and membrane proteins are synthesized. Correct folding of these ER client proteins...

... implications for the transparency, refractive function and pathophysiology of the tissue. * Author for correspondence (e-mail: [email protected] ) 2 2 2009 © The Company of Biologists Limited 2009 2009 Lens Green fluorescent protein Fluorescence correlation spectroscopy...

... osteoclast-like cells and live-cell confocal imaging was used to localize enhanced green fluorescent protein (EGFP)-tagged PKC. Benzoylbenzoyl-ATP (BzATP; a P2X7 agonist) induced transient translocation of PKCα to the basolateral membrane. UTP or ATP (10 μM), which activate P2 receptors other than P2X7...

... of Ca 2+ on receptor-triggered PLC activity in individual insulin-secreting β-cells. Evanescent wave microscopy was used to record PLC activity using green fluorescent protein (GFP)-tagged PIP 2 /IP 3 -binding pleckstrin homology domain from PLCδ1, and the cytoplasmic Ca 2+ concentration ([Ca 2+ ] i...

...-repressor SMRT Nuclear structure Green fluorescent protein Fluorescence microscopy Pit-1 Coactivator and co-repressor protein complexes control gene expression by modifying the acetylation states of histones and other transcriptional regulators (for reviews, see Orphanides and Reinberg, 2002...

... pathogenic factor in numerous diseases known as ER storage diseases. Here, we report that heterologous overexpression of the green fluorescent protein-tagged iodide transporter pendrin (GFP-PDS) perturbs folding and degradation processes in the ER. Pendrin (PDS) is a chloride-iodide transporter found...

... of mice carries a green fluorescent protein (GFP) transgene. In the lenses of hemizyous animals, GFP was expressed in a variegated fashion, allowing diffusion of GFP to be visualized directly. Early in development, GFP expression in scattered fiber cells resulted in a checkerboard fluorescence pattern...

... junctions Green fluorescent protein Membrane channels Oligomeric proteins Connexin subunit assembly Gap junctions are tightly packed arrays of membrane channels that mediate intercellular communication in multicellular organisms (reviewed in Bruzzone et al., 1996 ; Goodenough et al., 1996...

... of thyroid epithelial cells, we have expressed a chimeric protein consisting of rat cathepsin B and green fluorescent protein. Heterologous expression in CHO cells validated the integrity of the structural motifs of the chimeric protein for targeting to endocytic compartments. Homologous expression...

...Hyun-Hee Kong; Thomas D. Pollard We developed a reliable method for transient transfection of Acanthamoeba using Superfect (Qiagen) and a vector with the Acanthamoeba ubiquitin promoter and enhanced green fluorescent protein (EGFP) as the reporter gene. The transfection efficiency was 3...

... 2002 © The Company of Biologists Limited 2002 2002 Complex plastids Bipartite pre-sequence Diatom Green fluorescent protein Plastid import Diatoms (Bacillariophyceae) are a diverse and ubiquitous group of photoautotrophic, aquatic organisms. They are abundant primary producers...

..., is a component of the mechanism for gene regulation in vivo. * Author for correspondence (e-mail: [email protected] ) 5 8 2002 © The Company of Biologists Limited 2002 2002 Intranuclear targeting Runt homology factors Green fluorescent protein Fluorescence recovery after...

... filament Plectin 14-3-3 Live cell imaging Green fluorescent protein Intermediate filaments (IFs) of the cytokeratin (CK) type are major stabilising elements of the epithelial cytoskeleton( Fuchs and Cleveland, 1998 ; Coulombe and Omary, 2002 ). The numerous genodermatoses caused by CK gene...

...Marcel Tanudji; Sarah Hevi; Steven L. Chuck The green fluorescent protein is a cytosolic protein frequently used as a molecular tag to study protein localization in intact cells. We discovered that this protein is secreted into the medium by several but not all cell lines through a non-classical...

... accumulation in low-density cells and residues 78-119 were required for cytoplasmic localization in high-density cells. Fusion of inhibitor-2 residues 78-119 to green fluorescent protein was sufficient to produce cytoplasmic retention. Inhibitor-2 fused to triple tandem green fluorescent protein (100 kDa...

... analysed the interaction of mitochondria with microtubules in the filamentous fungus Neurospora crassa . Mitochondria were fluorescently labelled by expression of matrix-targeted green fluorescent protein. Upon isolation, mitochondria collapsed to round spherical structures that were still able to interact...

... for correspondence (e-mail: [email protected] ) 23 1 2002 © The Company of Biologists Limited 2002 2002 Cell contact Desmosome Intermediate filament Live cell imaging Green fluorescent protein Fluorescence recovery after photobleaching Cell-cell adhesion is mediated by various...

... cells expressing dynein intermediate chain-green fluorescent protein (IC-GFP) fusion in an IC-null background. Dynein-associated structures display fast linear movement along microtubules in both minus-end and plus-end directions, with velocities similar to that of dynein and kinesin-like motors...

... and cyan shifted green fluorescent proteins (yfp and cfp) is especially useful ( Ellenberg et al., 1999 ). Our previous study used a FN-green fluorescent protein (gfp) fusion protein to visualize the dynamics of the established FN matrix. We showed that some FN matrix fibrils are highly stretched...

... and little SNX1 was found in pellet fractions. Using green fluorescent protein fusions, endocytic compartment markers and fluorescence recovery after photobleaching, we found that there is an equilibrium between free cytoplasmic and early/sorting endosome-bound pools of green fluorescent protein-SNX1...