...Roma Dahara; Dibyendu Bhattacharyya Regulation of the distinctive stacked Golgi morphology remains an unresolved subject. Using budding yeast Pichia pastoris , we previously documented the role of GRIP domain Golgin Pp Imh1 in cisternal stacking, regulated by the Arl3-Arl1 GTPase cascade switch...

... availability. However, whether AMPK activity is spatially regulated, and the implications for cell function, have been unclear. We now report that AMPK associates with the Golgi, and that its activation by two specific pharmacological activators leads to Golgi fragmentation similar to that caused...

...Mitchell Leih; Rachael L. Plemel; Matt West; Cortney G. Angers; Alexey J. Merz; Greg Odorizzi ABSTRACT Vesicles bud from maturing Golgi cisternae in a programmed sequence. Budding is mediated by adaptors that recruit cargoes and facilitate vesicle biogenesis. In Saccharomyces cerevisiae , the AP-3...

...Lawrence G. Welch; Nadine Muschalik; Sean Munro ABSTRACT Golgi-resident enzymes remain in place while their substrates flow through from the endoplasmic reticulum to elsewhere in the cell. COPI-coated vesicles bud from the Golgi to recycle Golgi residents to earlier cisternae. Different enzymes...

... of the Golgi. Sal alters the expression of Golgi-related genes and leads to marked changes in Golgi morphology, particularly in cells that have undergone EMT. Moreover, Golgi-disturbing agents severely affect post-translational modifications of proteins, including protein processing, glycosylation...

... that is conserved in CerS proteins and functions through its non-catalytic activity. Tlc4 was localized at the NE and endoplasmic reticulum, similar to CerS proteins, and also showed unique additional localization at the cis- and medial-Golgi cisternae. Growth and mutation analyses revealed that Golgi localization...

... similarities, tethers have been classified into two groups – the coiled-coil tethers and the multisubunit tethering complexes (MTCs) ( Brunet and Sacher, 2014 ). Coiled-coil tethers are well-represented by golgin protein family proteins, which are anchored to the Golgi membrane ( Gillingham, 2018...

...Charlotte Ford; Anup Parchure; Julia von Blume; Christopher G. Burd ABSTRACT The Golgi functions principally in the biogenesis and trafficking of glycoproteins and lipids. It is compartmentalized into multiple flattened adherent membrane sacs termed cisternae, which each contain a distinct...

...Stefan Scholl; Stefan Hillmer; Melanie Krebs; Karin Schumacher ABSTRACT The trans -Golgi network/early endosome (TGN/EE) serves as the central hub in which exocytic and endocytic trafficking pathways converge and specificity of cargo routing needs to be achieved. Acidification is a hallmark...

..., the MIA3 gene encodes two major forms of transport and Golgi organization protein 1 (TANGO1S and TANGO1L), which have previously been implicated in selective trafficking of procollagen. Using genome engineering of human cells, light microscopy, secretion assays, genomics and proteomics, we show...

... that the spindle assembly factor TPX2 regulates astral microtubules. TPX2 in the spindle pole area is activated by GM130 (GOLGA2) on Golgi membranes to promote astral microtubule growth. GM130 relieves TPX2 inhibition by competing for importin α1 (KPNA2) binding. Mitotic phosphorylation of importin α at serine 62...

... the Golgi, with TRAPPIII also activating RAB1 in autophagosomes. Our finding that Aspergillus nidulans TRAPPII can be assembled by addition of a TRAPPII-specific subcomplex onto core TRAPP prompted us to investigate the possibility that TRAPPI and/or TRAPPIII already residing in the Golgi matures...

... have identified Golgi proteins as SIRT2 interactors, indicating that SIRT2 may also play a role in Golgi structure formation. Here, we show that SIRT2 depletion causes Golgi fragmentation and impairs Golgi reassembly at the end of mitosis. SIRT2 interacts with the Golgi reassembly stacking protein...

... complex Clathrin Membrane traffic Endocytosis Golgi Hereditary spastic paraplegia Wellcome Trust 10.13039/100004440 086598 214272 Nearly a third of the proteins encoded by the human genome are predicted to be integral membrane proteins, all of which have to get to the right...

...Bhawik Kumar Jain; Roma Dahara; Dibyendu Bhattacharyya ABSTRACT The adhesive force for cisternal stacking of Golgi needs to be reversible – to be initiated and undone in a continuous cycle to keep up with the cisternal maturation. Microscopic evidence in support of such a reversible nature...

...-translational modifications, H-Ras and N-Ras traffic to the PM from the Golgi via the classical exocytic pathway, but the exact mechanism of K-Ras trafficking to the PM from the ER is not fully characterized. ATP5G1 (also known as ATP5MC1) is one of the three proteins that comprise subunit c of the F 0 complex...

... of the Golgi depends on its association with a septin 1 (SEPT1)-based scaffold, which promotes local microtubule nucleation and positioning of the Golgi. SEPT1 function depends on the Golgi matrix protein GM130 (also known as GOLGA2) and on centrosomal proteins, including CEP170 and components of γ-tubulin...

...Vibha Singh; Chaitanya Erady; Nagaraj Balasubramanian ABSTRACT Cell-matrix adhesion regulates membrane trafficking controlling anchorage-dependent signaling. While a dynamic Golgi complex can contribute to this pathway, its regulation by adhesion remains unclear. Here we report that loss...

... show that VLDLR is required for mPR trafficking from the endoplasmic reticulum to the Golgi. Taken together, our data define a novel function for the VLDLR as a trafficking chaperone required for the mPR subcellular localization and, as such, non-genomic progesterone-dependent signaling. This article...

... as the most strongly upregulated gene from a comparative RNAseq analysis of cells in which expression of the Golgi matrix protein giantin had been abolished by gene editing. In contrast to previous work where we showed that depletion of giantin by RNAi results in defects in ciliogenesis and in cilia length...