... regulating GLUT4 trafficking downstream of different signaling inputs in a physiologically relevant cell type. APEX2 proximity biotinylation assays were performed as previously described ( Hung et al., 2016 ). Briefly, SKM cells at day 3 of differentiation stably expressing HA–GLUT4–APEX2 were incubated...

... in podocytes and attenuates glucose uptake, possibly due to a defect in the uncoating of clathrin-coated vesicles. GGA2 Glut4 trafficking Actin Clathrin Insulin resistance is a primary defect contributing to the development of type 2 diabetes mellitus. It can be due to defects in the insulin...

.... , Chen Y. , Ji W. , Qu A. , Xu P. , James D. E. , Xu T. ( 2007 ). Dissecting multiple steps of GLUT4 trafficking and identifying the sites of insulin action . Cell Metab. 5 , 47 - 57 . Bao Y. , Lopez J. A. , James D. E. , Hunziker W. ( 2008...

... to be consistent with the possibility that the separate signals lead to a single point of convergence with the GLUT4 trafficking pathway. Recent signalling studies in skeletal muscle have led to the postulation that the Rab-GAP proteins AS160 and TBC1D1 act as points of convergence for signalling from insulin...