...), and whole-mount immunofluorescence (WMIF). We applied IBEX at multiple stages to study the spatial and temporal relationships between glia and neurons during retinal development. Finally, we demonstrate the utility of IBEX across species by testing it on the turquoise killifish ( Nothobranchius furzeri...

...Raquel Pérez-Gómez; Jana Slováková; Noemí Rives-Quinto; Alena Krejci; Ana Carmena Summary It is firmly established that interactions between neurons and glia are fundamental across species for the correct establishment of a functional brain. Here, we found that the glia of the Drosophila larval...

.... Downregulation of either ogre or inx2 selectively in glia, by targeted expression of RNA interference transgenes, leads to a significant reduction in the size of the larval nervous system and behavioural defects in surviving adults. We conclude that these innexins are crucially required in glial cells for normal...

... Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA ‡ Author for correspondence ( [email protected] ) 15 3 2011 © 2011. 2011 Subependymal zone Rostral migratory stream Neuroblast Glia Microglia Gal3 -null mutant mice Thousands of neurons...

... The Company of Biologists Limited 2006 2006 Connexin43 Oculodentodigital dysplasia (ODDD) Hemichannel Gap junction Glia Connexins oligomerize to form gap junctions ( Nicholson, 2003 ; Saez et al., 2003 ). Gap junctions allow direct intercellular communication between adjacent cells via...

... ( Fig. 3D 1 ). This observation suggested quantal transmitter release at a neuron-glia synapse-like structure and predicted the existence of spontaneous glial PSPs. Indeed, low-frequency, spontaneous PSPs (sPSPs) ( Fig. 3A 3 , bottom) occurred in most GluR cells tested (20/24). Fig. 2. Post...

.... Immunofluorescence and immunoelectron microscopy revealed that Necl-1 localized at the contact sites among axons, their terminals, and glia cell processes that cooperatively formed synapses, axon bundles and myelinated axons. Necl-1 showed Ca 2+ -independent homophilic cell-cell adhesion activity. It furthermore...