... Arl1p and results in less Arl1p residing on the TGN. Biochemically, purified Imh1p competes with Gcs1p, an Arl1p GTPase-activating protein (GAP), for binding to Arl1p, thus interfering with the GAP activity of Gcs1p toward Arl1p. Furthermore, we demonstrate that the self-interaction of Imh1p attenuates...

... intrinsic GTPase activity; consequently GTPase-activating proteins (GAPs) are crucial for Arf function ( Randazzo and Kahn, 1994 ; Nie et al., 2003 ). The ArfGAPs are a family of proteins with diverse domain structures ( Randazzo and Hirsch, 2004 ; Turner et al., 2001 ). Twenty-four genes encoding...

... was originally linked to the assembly and disassembly of vesicle coats. Arf GTPase-activating proteins (GAPs), a family of proteins that induce hydrolysis of GTP bound to Arf, were therefore proposed to regulate the disassembly and dissociation of vesicle coats. Following the molecular identification of Arf GAPs...

... inhibiting R-Ras function through phosphorylation, Eph receptors can reduce R-Ras activity through the GTPase-activating protein, p120RasGAP. By using R-Ras mutants that cannot be inactivated by p120RasGAP and/or cannot be phosphorylated at tyrosine 66, we show that the two forms of R-Ras negative regulation...

... for correspondence (e-mail: [email protected] ) 24 10 2005 © The Company of Biologists Limited 2006 2006 GTPase-activating protein ARAP3 PI3K Rho Arf Lamellipodium Many cells respond to appropriate signals by forming lamellipodia, dynamic, sheet-like membrane protrusions...

[email protected] ) 17 8 2005 © The Company of Biologists Limited 2005 2005 Cytokinesis RacGAP Drosophila Rho-GTPase signalling Centralspindlin GTPase-activating protein After chromosomes have moved to the poles of the mitotic spindle in a dividing cell, a complex set of cellular...