...Inga Mohr; Amin Mirzaiebadizi; Sibaji K. Sanyal; Pichaporn Chuenban; Mohammad R. Ahmadian; Rumen Ivanov; Petra Bauer ABSTRACT Small GTPases switch between GDP- and GTP-bound states during cell signaling. The ADP-ribosylation factor (ARF) family of small GTPases is involved in vesicle trafficking...

...Christiane Zhu; Susanne Herbst; Patrick A. Lewis ABSTRACT Leucine-rich repeat kinase 2 (LRRK2) is a multidomain scaffolding protein with dual guanosine triphosphatase (GTPase) and kinase enzymatic activities, providing this protein with the capacity to regulate a multitude of signalling pathways...

... engagement triggers rapid GTP-loading of the small GTPase Rac as a master regulator of cytoskeletal rearrangements and lamellipodia-driven internalization. To identify components of the CEACAM3-initiated signaling cascade, we performed a genome-wide CRISPR/Cas9-based screen in human myeloid cells. Following...

...: This study reveals a noncanonical role of AMP-activated kinase in which the α subunit C-terminal regulatory domain serves as a guanine-nucleotide-exchange factor for Arf6 during glucose deprivation. ADP-ribosylation factor GTPase Glucose deprivation Cell invasion Ministry of Education http...

... the recruitment of 5-inositol phosphatases to circular ruffles. Macropinocytosis GTPase Rab Phosphatase Phosphoinositide EGF Ruffling Canadian Institutes of Health Research http://dx.doi.org/10.13039/501100000024 FDN-143202 Heart and Stroke Foundation of Canada http...

...Kuan-Jung Chen; Tsai-Chen Chiang; Chia-Jung Yu; Fang-Jen S. Lee ABSTRACT Cell migration requires the coordination of multiple signaling pathways involved in membrane dynamics and cytoskeletal rearrangement. The Arf-like small GTPase Arl4A has been shown to modulate actin cytoskeleton remodeling...

...Marita Borg Distefano; Linda Hofstad Haugen; Yan Wang; Harmonie Perdreau-Dahl; Ingrid Kjos; Da Jia; Jens Preben Morth; Jacques Neefjes; Oddmund Bakke; Cinzia Progida ABSTRACT Rab GTPases are key regulators of intracellular trafficking, and cycle between a GTP-bound active state and a GDP-bound...

.... This recruitment requires the HDS1 and HDS2 domains. Golgi Arf GTPase GBF1 Membrane trafficking Regulation Canadian Institutes of Health Research 10.13039/501100000024 FRN 111028 Faculty of Medicine and Dentistry Faculty of Graduate Studies and Research...

...Chia-Jung Yu; Fang-Jen S. Lee ABSTRACT ADP-ribosylation factors (Arfs) and ADP-ribosylation factor-like proteins (Arls) are highly conserved small GTPases that function as main regulators of vesicular trafficking and cytoskeletal reorganization. Arl1, the first identified member of the large Arl...

... the more prominent role. Using a proximity-based biotin labeling approach, we found close associations between MiD51, Mff and Drp1, but not Fis1. Furthermore, we found that MiD51 can suppress Mff-dependent enhancement of Drp1 GTPase activity. Our data indicates that Mff and MiD51 regulate Drp1 in specific...

... to its focus in cancer studies – there is significant KRAS4A expression in some tissues ( Tsai et al., 2015 ). RAS GTPases cycle between the GDP-bound inactive and GTP-bound active states with the help of guanine nucleotide exchange factors (RASGEFs) that promote activation, and GTPase-activating...

...Bertha C. Elias; Amrita Das; Diptiben V. Parekh; Glenda Mernaugh; Rebecca Adams; Zhufeng Yang; Cord Brakebusch; Ambra Pozzi; Denise K. Marciano; Thomas J. Carroll; Roy Zent ABSTRACT The Rho GTPase Cdc42 regulates key signaling pathways required for multiple cell functions, including maintenance...

...Yan Zhen; Harald Stenmark ABSTRACT Rab GTPases control intracellular membrane traffic by recruiting specific effector proteins to restricted membranes in a GTP-dependent manner. In this Cell Science at a Glance and the accompanying poster, we highlight the regulation of Rab GTPases by proteins...

...Graham M. Burkart; Tobias I. Baskin; Magdalena Bezanilla ABSTRACT In plants, the ROP family of small GTPases has been implicated in the polarized growth of tip-growing cells, such as root hairs and pollen tubes; however, most of the data derive from overexpressing ROP genes or constitutively active...

... growth is determined in mid G1. Cdc42 polarization in the first step and its proper positioning depend on Rsr1 and its GTPase-activating protein (GAP) Bud2. Interestingly, Rga1, a Cdc42 GAP, exhibits transient localization to a site near the bud neck and to the division site during cytokinesis and G1...

... GTP-bound Rap1. This effect was significantly reduced in cells that were either transfected with DOK1 siRNAs ( Fig. 6A ) or that overexpressed the non-phosphorylatable p130Cas15F mutant ( Fig. 6B ) ( Evans et al., 2011 ) compared with controls. Because the Rac1 GTPase has also been shown to become...

...Jia-Wei Hsu; Zzu-Jung Chen; Ya-Wen Liu; Fang-Jen S. Lee ABSTRACT Small GTPase ADP-ribosylation factors (ARFs) are key regulators of membrane trafficking and their activities are determined by guanine-nucleotide-binding status. In Saccharomyces cerevisiae , Arl1p, an ARF-like protein, is responsible...

... 2013 15 10 2013 © 2014. Published by The Company of Biologists Ltd 2014 Golgi Arf GTPase GBF1 Brefeldin A The Golgi functions as a central organizing organelle in the secretory pathway ( Farquhar and Palade, 1998 ; Emr et al., 2009 ). The central Golgi stack (consisting...

...Joshua S. Chappie; Fred Dyda Summary Dynamin is a large multidomain GTPase that assembles into helical arrays around the necks of deeply invaginated clathrin-coated pits and catalyzes membrane fission during the final stages of endocytosis. Although it is well established that the function...

...Ki Lui; Jie An; JoAnne Montalbano; Jingxue Shi; Chad Corcoran; Qin He; Hong Sun; M. Saeed Sheikh; Ying Huang Summary We had previously reported that RBEL1A, a novel Ras-like GTPase, was overexpressed in multiple human malignancies and that its depletion suppressed cell growth. However...